Facial repair injection based on PRP and stem cell exosome and preparation method thereof
A facial repair and stem cell technology, applied in the field of stem cell application, can solve the problems of insufficient secretion, affecting the application effect of stem cell exosomes, etc., to achieve the effect of increasing secretion, avoiding the risk of bacterial infection, and repairing the traces of time
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Embodiment 1
[0034] This embodiment provides a facial repair injection based on PRP and stem cell exosomes and a preparation method thereof, so as to increase the secretion amount thereof for skin anti-aging, repair and disease treatment.
[0035] In order to achieve the above purpose, this embodiment provides a facial repair injection based on PRP and stem cell exosomes, including 1 part of platelet-rich plasma, 2 to 5 parts of stem cell exosomes, and 4 to 7 parts of normal saline by volume.
[0036] Further, the facial repair injection based on PRP and stem cell exosomes includes 1 mL of platelet-rich plasma, 3 mL of stem cell exosomes, and 6 mL of normal saline by volume.
[0037] Further, the described facial repair injection based on PRP and stem cell exosomes includes, by volume, 1 mL of platelet-rich plasma, 2 mL of stem cell exosomes, and 7 mL of normal saline.
[0038] Further, the described facial repair injection based on PRP and stem cell exosomes includes, by volume, 1 mL of p...
Embodiment 2
[0056] Preparation of PRP and Stem Cell Exosome Facial Repair Fluid.
[0057] 1. Recipe
[0058] 1mL platelet-rich plasma, 3mL stem cell exosomes, 6mL normal saline.
[0059] 2. Preparation method
[0060] First, take the well-grown hUC-MSCs of the P3 generation, culture them in serum-free medium, collect the cells, wash them, resuspend in serum-free medium, collect the supernatant, and then centrifuge at 1200 rpm for 10 min and 1800 rpm for 20 min to collect the cells again. Then, the precipitate was collected by centrifugation at 150,000 rpm with an ultracentrifuge for 1.5 h, and the precipitate was resuspended with an appropriate amount of normal saline. Next, draw 10 mL of peripheral blood at the same time, and mix up and down. Transfer to a centrifuge tube for centrifugation, centrifuge at 2500rpm for 10min, suck the upper plasma and prp layer into the centrifuge tube and centrifuge again, centrifuge at 3200rpm for 8min, discard the upper layer of plasma and the lower ...
Embodiment 3
[0062] Facial cosmetic repair injection facilitates facial skin improvement application. like figure 1 , figure 2 Forehead 1, eyebrow arch 2, temple 3, lying silkworm 4, apple muscle 5, nose 6, lips 7, chin 8, forehead wrinkles 9, eyebrow wrinkles 10, eye wrinkles 11, crow's feet 12. Cheek depression 13, nasolabial groove 14, lip wrinkles 15, nasolabial folds 16, neck wrinkles 17, lip bead point 18, chin-labial groove 19, chin lower 20, hairline 21, brow point 22, under the nose Point 23 and other parts, according to the actual situation, use a microneedle to inject facial cosmetic repair injection.
[0063] When the PRP and stem cell exosome facial cosmetic repair injection of the present invention is introduced into the deep epidermis and dermis layer by using a microneedle, the human facial skin improvement experiment is carried out. The specific implementation steps are as follows: disinfect the skin with 75% medical alcohol, use a disposable For microneedling, take an...
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