Anticancer peptide DMS-PS1 of South America frog and application thereof

A technology of DMS-PS1 and anti-cancer peptides, which is applied in the fields of application, peptide sources, anti-tumor drugs, etc., can solve the problems of the limitations of the main methods, the strong drug resistance of chemotherapy drugs, side effects, etc.

Active Publication Date: 2022-08-02
WENZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Despite significant advances in cancer treatment in recent years, the main modalities currently used for cancer treatment are still limited
In addition, there are more and more studies and applications of cancer-targeted drugs, but chemotherapy drugs often have the disadvantages of strong toxic side effects and easy drug resistance.

Method used

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  • Anticancer peptide DMS-PS1 of South America frog and application thereof
  • Anticancer peptide DMS-PS1 of South America frog and application thereof
  • Anticancer peptide DMS-PS1 of South America frog and application thereof

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Experimental program
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Embodiment

[0015] 1. Identification of natural anticancer peptide DMS-PS1

[0016] 1. Preparation and amino acid sequence determination of natural anticancer peptide DMS-PS1

[0017] (1) Collection of frog skin secretions

[0018] After cleaning the surface of the frog skin with sterile water, gently rinse the back and ears with sterile water, and rinse the milky-white frog skin secretions on the surface into a beaker. This step can be performed in small quantities and several times. The frog's back skin can also be stimulated intermittently with a mild voltage of 15V for 5s to obtain frog skin secretions multiple times.

[0019] (2) Isolation and purification of natural anticancer peptide DMS-PS1

[0020] 5 mg of frog skin secretions were weighed and dissolved in 1 mL of 0.05% TFA aqueous solution (99.95% ultrapure water + 0.05% TFA), and centrifuged at 12000 rpm for 10 min after mixing. The supernatant was filtered and sonicated to remove air bubbles. Analysis of the supernatant wa...

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Abstract

The invention discloses a South America frog anti-cancer peptide DMS-PS1 and application thereof, the South America frog anti-cancer peptide DMS-PS1 can be used in the field of anti-cancer drugs and health care products and preparation, the novel natural anti-cancer peptide DMS-PS1 is separated from frog skin secretions by taking South America frog Phy l lomeduasauvagi as a material, and the novel natural anti-cancer peptide DMS-PS1 can be used for preparing anti-cancer drugs and health care products. The open reading frame of the polypeptide is composed of 76 amino acids, contains highly conservative signal peptide composed of 22 amino acid residues, is rich in aspartic acid, glutamic acid and other acidic amino acids, has a molecular weight of 2931.43 Daltons, contains amidation modification at the C-terminal, is an amphiphilic cationic peptide with an alpha-helical structure, and has a molecular weight of 2931.43 Daltons. The natural anti-cancer peptide DMS-PS1 disclosed by the invention shows spectral anti-cancer activity and shows certain killing selectivity to prostate cancer cells, and research finds that the natural anti-cancer peptide DMS-PS1 has an anti-cancer effect by inducing PC-3 cell apoptosis, so that a potential medicine is provided for cancer treatment.

Description

technical field [0001] The invention relates to the field of anticancer drugs and preparation thereof, in particular to a South American tree frog anticancer peptide DMS-PS1 and its application. Background technique [0002] The rapid development of modern medical care, science and technology and economy has brought about tremendous changes in people's lifestyles. The prolongation of life expectancy and environmental pollution have also increased the morbidity and mortality of malignant tumors, becoming the second most important threat to human life. major disease. According to the results of the Global Cancer Report, by 2020, there will be approximately 19.3 million malignant tumor cases worldwide and approximately 10 million deaths. Although significant progress has been made in cancer treatment in recent years, the main modalities currently used for cancer treatment are still limited. In addition, there are more and more researches and applications of cancer-targeted dr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/46C12N15/12A61K38/17A61P35/00
CPCC07K14/463A61P35/00A61K38/00Y02A50/30
Inventor 高艺恬郭允峰罗晓焱吴迪李晓坤
Owner WENZHOU UNIVERSITY
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