Tobacco essence characteristic compound analysis method and application thereof

A technology of characteristic compounds and analysis methods, applied in the field of accurate analysis of characteristic compounds of tobacco flavors, can solve problems such as differences in compatibility of tobacco leaves, lack of a general method, etc., and achieve the effect of improving accuracy and precision

Pending Publication Date: 2022-08-02
CHINA TOBACCO JIANGSU INDAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The difference in water-soluble sugar content in tobacco of different varieties, parts and origins also leads to significant differences in the compatibility of tobacco leaves
At present, the detection method of water-soluble sugar in tobacco is relatively mature, but there is no general method for the detection of characteristic components in tobacco products such as tobacco flavors

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The present embodiment provides a method for analyzing characteristic compounds of tobacco flavors, and the specific steps are as follows:

[0029] Weigh 0.2 g of the sample to be tested, accurate to 0.0001 g, and place it in a 10 mL centrifuge tube. Accurately add 0.2 mL of palmitic acid-d 31 The solution (5 g / L) and 5 mL of methanol were vortexed for 20 min, passed through a 0.22 μm organic filter membrane, and then 100 μL was taken and dried with nitrogen. Then add 30 μL 20 mg / mL pyridine solution of methoxyamine hydrochloride and 10 μL 18 mg / mL hydroxylamine hydrochloride in ethanol, vortex for 2 min, react at 30°C for 100 min, add 30 μL BSTFA, 30 μL MSTFA, 30 μL MTBSTFA, vortex After spinning for 30s, the reaction was carried out at 37°C for 60min. Finally, the derivatized solution was transferred to a microsampler for GC-MS analysis.

[0030] Column size: 30m×0.25mm×0.25μm, stationary phase is (5%-phenyl)-methyl polysiloxane; carrier gas: helium (4.1); inlet te...

Embodiment 2

[0035] This example provides a method for analyzing characteristic compounds of tobacco flavors. The specific steps are the same as those in Example 1, except that BSTFA is not added, and the reduced part is allocated to MSTFA and MTBSTFA in proportion.

Embodiment 3

[0037] This example provides a method for analyzing characteristic compounds of tobacco flavor. The specific steps are the same as those in Example 1, except that MSTFA is not added, and the reduced part is allocated to BSTFA and MTBSTFA in proportion.

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PUM

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Abstract

The invention provides a tobacco flavor characteristic compound analysis method and application thereof. The tobacco flavor characteristic compound analysis method comprises the following steps: mixing a to-be-detected sample with an internal standard substance, blow-drying, carrying out a mixed reaction with an oximation reagent, carrying out a mixed reaction with a silanization reagent, and finally carrying out GC-MS detection to obtain a result, the oximation reagent is a combination of methoxyamine hydrochloride and hydroxylamine hydrochloride. The analysis method provided by the invention can be used for efficiently and quickly detecting the characteristic compounds in the tobacco essence, and is accurate in detection result and high in precision.

Description

technical field [0001] The invention belongs to the field of analysis, and in particular relates to a method for analyzing characteristic compounds of tobacco flavor and its application, in particular to an accurate method for analyzing characteristic compounds of tobacco flavor and its application. Background technique [0002] The characteristic compounds of tobacco flavor are mainly water-soluble sugars. Water-soluble sugars are important primary metabolites in the process of tobacco growth and development. They are not only directly related to the photosynthesis and respiration of tobacco, but also directly affect the quality and quality of tobacco. The content of water-soluble sugars is closely related to the aroma, irritation and tar production of tobacco. For example, water-soluble reducing sugars and amino compounds can be burned to produce furan-like Maillard reaction products, which can effectively improve the quality of tobacco and reduce lignin. Miscellaneous ga...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72G01N30/06G01N30/30G01N30/60
CPCG01N30/02G01N30/7206G01N30/06G01N30/30G01N30/6052G01N2030/067G01N2030/045G01N2030/025
Inventor 马梦婕李炜付金存韩云龙郭宏霞刘琪袁益来朱莹
Owner CHINA TOBACCO JIANGSU INDAL
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