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Biomaterial-based compositions for delivery of microorganisms that promote plant growth

A technology of biological materials and compositions, applied in the directions of plant growth regulators, plant growth regulators, chemicals for biological control, etc., can solve the problems of long-term preservation of coated seeds, loss of microbial vitality, etc., and reduce moisture content. Intimidation, health-increasing effects

Pending Publication Date: 2022-08-02
MASSACHUSETTS INST OF TECH
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

In fact, incorporation of inoculum into artificial seed coats resulted in loss of microbial viability and long-term storage of coated seeds was not possible (12)

Method used

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  • Biomaterial-based compositions for delivery of microorganisms that promote plant growth
  • Biomaterial-based compositions for delivery of microorganisms that promote plant growth
  • Biomaterial-based compositions for delivery of microorganisms that promote plant growth

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Experimental program
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Embodiment

[0056] Materials and methods

[0057] MD simulation -(GAGSGA) 2 The initial model of the oligomer was constructed by threading the dodecapeptide sequence onto the structure of the poly-(glycine-alanine)b-sheet (Protein Database ID 2slk) using the UCSF chimera and modeler. The system was then equilibrated in a transferable intermolecular potential 3P (TIP3P) explicit water tank. Simulations were run for 40 ns with a time step of 2 fs at constant temperature (300 K) and pressure (1 bar) using GROMACS. The force field used is GROMOS53a6. The α,α-trehalose structure was provided by the Automatic Topology Generator (ATB) and a repository available from the Molecular Dynamics Group at the University of Queensland, Queensland, Australia. The stability of the β-sheet assembly was verified from hydrogen bonding dynamics and root mean square deviation (RMSD) data obtained from molecular dynamics trajectories.

[0058] Material manufacturing - To study the biopolymer preservation...

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Abstract

Herbicides, insecticides, and synthetic fertilizers may be replaced with bio-fertilizers in combination with microorganisms. Silk and trehalose dry films can be used as seed coatings to locate and quantify delivery of plant microorganisms to mitigate plant stress and soil salinity. Similar microorganisms may be delivered using the same technique.

Description

[0001] Related applications [0002] This application claims the benefit of US Provisional Application No. 62 / 924,483, filed October 22, 2019, and US Provisional Application No. 63 / 036,088, filed June 8, 2020. The entire teachings of the above applications are incorporated herein by reference. Background technique [0003] In a world struggling to adapt to population growth and changing climate patterns, there is an urgent need to develop new technologies to increase agricultural output while minimizing inputs and mitigating its environmental impact. Population growth, soil degradation and misuse of agrochemicals are the main challenges that agriculture will need to face in the coming decades to meet the growing demands in global food production. In this context, seed fortification technologies will play a key role in supporting food security by enabling seeds to germinate in degraded environments, increasing yields and reducing germination time. So far, great efforts have b...

Claims

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Application Information

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IPC IPC(8): A01N25/04A01N63/20A01N37/46A01N43/16A01P21/00A01C1/06
CPCA01N25/00A01N63/20
Inventor 贝内代托·马瑞利奥古斯丁·兹威尼威士
Owner MASSACHUSETTS INST OF TECH
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