Staphylococcus aureus New Zealand white rabbit bone infection animal model construction method
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A staphylococcal, animal model technology, applied in the field of bone infection, can solve the problems of less evidence, less stable method, limited scope of use, etc., and achieve the effect of easy operation, short modeling time, simple and effective operation
Pending Publication Date: 2022-08-05
THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV
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Problems solved by technology
The use of New Zealand white rabbits to establish a bone infection model has little basis, the method is not stable, and the fit with the clinical characteristics of infectious diseases is low, so the scope of use is limited
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[0027] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.
[0028] see Figure 1-4 , the present invention provides a kind of technical scheme:
[0029] The method for constructing an animal model of Staphylococcus aureus-induced New Zealand white rabbit bone infection includes the following steps:
[0030] a. Select New Zealand white rabbits, among which New Zealand white rabbits are New Zealand female white rabbits of 8-12 weeks and weighing 2-2.5 kg;
[0031] b. Select titanium alloy scre...
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Abstract
The invention discloses a staphylococcus aureus New Zealand white rabbit bone infection animal model construction method. The method comprises the following steps: a, selecting a New Zealand white rabbit; b, selecting a titanium alloy screw, and pretreating the titanium alloy screw; c, a longitudinal incision with the length of 1 cm is formed in the position, 2 cm away from the inner side below the left knee joint, of the New Zealand white rabbit, and bone cortex is cut open layer by layer; d, a drill bit with the diameter being 2 mm is selected, a hole is formed in the bone cortex, the titanium alloy screw polluted by the staphylococcus aureus is implanted into the bone tunnel, incisions are sutured layer by layer, and sterile binding is conducted; e, CT iconography data are obtained, and the conditions of bone destruction, soft tissue swelling and the like around the titanium alloy screw are observed; and f, taking out the screw, carrying out scanning electron microscope detection, and carrying out histopathologic staining. The method is simple, effective, convenient to operate and short in modeling time, can serve as a perfect and feasible bone infection biological model, and can provide an experimental basis for exploration of clinical bone infection pathogenesis, bone infection treatment ways and evaluation of the effect and safety of new drug development.
Description
technical field [0001] The invention relates to the technical field of bone infection, in particular to a method for constructing an animal model of Staphylococcus aureus-induced New Zealand white rabbit bone infection. Background technique [0002] Implant-related infection is one of the catastrophic complications of orthopaedics. It is difficult to treat, easy to relapse, has poor curative effect, and has a long treatment cycle. In severe cases, it can lead to amputation or death. According to reports, the incidence of bone infection is 3%-5%, but the recurrence rate is as high as 40%. The main pathogen causing plant-related infection is Staphylococcus aureus. One of the reasons why bone infections are difficult to treat is the potential of Staphylococcus aureus to form bacterial biofilms. Because antibiotics have difficulty penetrating bacterial biofilms and cannot kill bacteria, bacterial biofilms are protective devices that aid bacterial immune escape. [0003] In rec...
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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027
CPCA01K67/027A01K2207/30A01K2227/107A01K2267/0337
Inventor 任有亮杨晋楚磊邓忠良金瑛
Owner THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV
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