Application of miRNA in preparation of echinococcosis risk assessment or early screening reagent

A technology for risk assessment and echinococcosis, applied in the field of medicine, achieves the effects of good stability, high sensitivity and specificity, and good diagnostic effect

Pending Publication Date: 2022-08-05
NANJING MEDICAL UNIV
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Problems solved by technology

However, whether miRNA targeting substances necessary for echinococcosis growth can be used as biomarkers for echinococcosis risk assessment has not been reported so far

Method used

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  • Application of miRNA in preparation of echinococcosis risk assessment or early screening reagent
  • Application of miRNA in preparation of echinococcosis risk assessment or early screening reagent
  • Application of miRNA in preparation of echinococcosis risk assessment or early screening reagent

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Embodiment Construction

[0029] The present invention will be described in further detail below in conjunction with the embodiments. If the reagents or equipment used are not marked with the manufacturer, they are regarded as conventional products that can be purchased through the market.

[0030] The inventors collected serum from 8 patients with echinococcosis granulosa etiologically confirmed patients (ie, patients with hydatid disease) from the Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University, and 8 healthy controls provided by the echinococcosis laboratory of Ningxia Medical University. serum.

[0031] 1. Experimental method:

[0032]1. Use miRNeasy Serum / Plasma Kit to extract total RNA from serum of hydatid patients and healthy controls. The specific operation is as follows: after centrifuging the serum at 3000g for 5 minutes, add 1ml TRIzol and mix by pipetting. Let stand for 10 minutes, add 200ul of chloroform, invert vigorously and mix for 30 seconds, let...

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Abstract

The invention belongs to the field of medicines, and particularly relates to application of miRNA in preparation of an echinococcosis risk assessment or early screening reagent. The invention relates to a serum miRNA (micro Ribonucleic Acid) marker related to risk assessment or early screening of echinococcosis. The serum miRNA marker is one or more of hsa-miR-516a-5p, hsa-miR-516b-5p, hsa-miR-518e-5p, hsa-miR-519a-5p, hsa-miR-519b-5p, hsa-miR-522-5p, hsa-miR-523-5p, hsa-miR-6852-5p, hsa-miR-1304-3p, hsa-miR-3138, hsa-miR-431-5p, hsa-miR-324-3p, hsa-miR-526b-5p and hsa-miR-5187 As a novel biomarker, the miRNA disclosed by the invention is good in stability and high in sensitivity and specificity.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the application of a miRNA targeting the synthesis pathway of essential substances for hydatid growth in the preparation of hydatid disease risk assessment or early screening reagents. Background technique [0002] Echinococcus granulosus, or hydatid disease, is an important zoonotic parasitic disease caused by Echinococcus granulosus infection of humans. Echinococcus granulosus infection has no obvious symptoms in the early stage, and patients usually do not go to the hospital for treatment. However, the development of hydatid disease to the chronic stage can manifest as cysts in important organs such as liver and lung, and the mortality rate is as high as 2%-4% (McManus DP, ZhangW, Li J, Bartley PB. Echinococcosis. Lancet. 2003; 3629392:1295 -304; doi:10.1016 / S0140-6736(03)14573-4). If effective treatment measures are not taken in time, the mortality of patients can be sig...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6888C12N15/11
CPCC12Q1/6883C12Q1/6888C12Q2600/178Y02A50/30
Inventor 王海苏川戴俊程刘巧玉陈晓军马子健
Owner NANJING MEDICAL UNIV
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