Preparation method of molecularly imprinted polymer based on curcumin, electrochemical sensor and application thereof

A technology of molecular imprinting and curcumin, applied in the field of detection, can solve problems such as affecting the universal applicability of experimental methods

Pending Publication Date: 2022-08-09
CHONGQING MIDECO PHARMA CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, even the sample pretreatment methods are diverse, which results in the accuracy and precision of sub

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  • Preparation method of molecularly imprinted polymer based on curcumin, electrochemical sensor and application thereof
  • Preparation method of molecularly imprinted polymer based on curcumin, electrochemical sensor and application thereof
  • Preparation method of molecularly imprinted polymer based on curcumin, electrochemical sensor and application thereof

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Experimental program
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preparation example Construction

[0030] The present application prepares a biocompatible material that is easy to extract and separate curcumin through the self-polymerization of zein, which can effectively extract curcumin from any environment and raw material such as high temperature, low temperature, high pressure, and a large amount of organic solvents A novel molecularly imprinted polymer (MBMIP) for highly sensitive and selective determination of curcumin in complex samples was successfully constructed by combining zein with a curcumin template. As a functional monomer and cross-linking agent, zein reacts with templates to form microsphere polymers under mild conditions; zein can self-polymerize by changing the polarity of the solvent, thereby introducing magnetic Fe 3 O 4 Nanoparticles, molecularly imprinted polymers with specific adsorption and magnetic properties are obtained. Specifically, the embodiment of the present invention discloses a preparation method of a curcumin-based molecularly imprint...

Embodiment 1

[0051] 1Materials and Instruments

[0052] Curcumin (98%) was purchased from China Institute of Pharmaceutical and Biological Products, ferric chloride hexahydrate (FeCl 3 ·6H 2 O), anhydrous sodium acetate (NaAc), ethylene glycol (CH 2 OH) 2 , sodium citrate (Na 3 C 6 H 5 O 7 ·2H 2 O), hydrochloric acid (HCl), methanol (MeOH), anhydrous ethanol (EtOH), and acetic acid (AcOH) were purchased from Chengdu Kelong Chemical Reagent Factory (Chengdu), and acetonitrile (ACN) and methanol were obtained from Merck ) produced high performance liquid chromatography (HPLC) grade products, and the ultrapure water used in the experiment was prepared by the Millipore-q system of Millipore, Milford, MA, USA. All other reagents were analytical grade.

[0053] Scanning electron microscope (SEM) and transmission electron microscope (TEM) images were obtained with MERLIN Compact (ZEISS, Germany) and JEM 2100F (JEOL, Japan). Infrared spectra were obtained by near-infrared affinity Fourie...

Embodiment 2

[0068] Example 2 Verification and Analysis

[0069] 1 SEM and TEM patterns of MBMIPs, MBNIPs and Fe3O4NPs

[0070] MBMIPs ( figure 2 a), MBNIPs ( figure 2 b) and Fe 3 O 4 NPs( figure 2 c) The morphological features were characterized, such as figure 2 shown, by figure 2 It can be seen that Fe 3 O 4 The NPs are spherical, highly monodisperse, and have a diameter of 300 nm; the MBMIPs ( figure 2 d), MBNIPs ( figure 2 e) and Fe 3 O 4 NPs( figure 2 f) were characterized by their morphological features; scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images showed that the MBMIPs were spherical structures with a diameter of 300–380 nm, compared with Fe 3 O 4The particle size of NPs increased significantly, it is speculated that zein has been successfully polymerized in Fe. 3 O 4 on the surface.

[0071] 2 Fe 3 O 4 FT-IR spectra of NPs, Zein and MBMIPs

[0072] FT-IR analysis confirmed the presence of zein in the nanoparticl...

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Abstract

The invention provides a curcumin-based molecularly imprinted polymer preparation method, which comprises: mixing curcumin, an organic solvent and water to obtain a mixed solution; mixing magnetic Fe3O4 nanoparticles, zein and the mixed solution, and carrying out auto-polymerization to obtain an initial polymer; and cleaning the initial polymer to remove curcumin so as to obtain the curcumin-based molecularly imprinted polymer. The invention further provides an electrochemical sensor based on the molecularly imprinted polymer of curcumin and application based on the electrochemical sensor. The electrochemical sensor provided by the invention is based on specific adsorption of the molecularly imprinted polymer, so that the electrochemical sensor has high efficiency, rapidness and accuracy when being used for detecting curcumin.

Description

technical field [0001] The invention relates to the technical field of detection methods, in particular to a preparation method of a curcumin-based molecularly imprinted polymer, an electrochemical sensor and applications thereof. Background technique [0002] Curcumin is a chemical component extracted from the rhizomes of some plants in the Zingiberaceae and Araceae. Among them, turmeric contains about 3% to 6%, which is a very rare pigment with diketones in the plant kingdom. It is a diketone. compound. The antioxidant or prooxidative activity of curcumin depends on its concentration, so it is crucial to control the concentration of curcumin in food entering the human body, so it is very necessary to develop an accurate and simple assay to detect curcumin. [0003] The detection or collection of trace substances from the environment, food, biological samples and natural products has always been the focus and difficulty of current research in various fields. Researchers i...

Claims

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Application Information

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IPC IPC(8): C08J9/26C08L89/00C08K3/22G01N27/30G01N27/48
CPCC08J9/26G01N27/308G01N27/48C08K2201/01C08K2003/2275C08J2389/00
Inventor 张起辉李伟
Owner CHONGQING MIDECO PHARMA CO LTD
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