Unlock instant, AI-driven research and patent intelligence for your innovation.

Cymbidium quick-acting breeding method

A fast, plant-based technology, applied in the field of plant tissue culture, can solve problems such as destruction of wild resources and endangerment, and achieve the effects of easy method, convenient operation and fast reproduction.

Inactive Publication Date: 2004-10-06
中国科学院武汉植物研究所
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because people in order to obtain novel treasures, at the cost of destroying resources, they dig indiscriminately and indiscriminately, resulting in extremely serious damage to wild resources, and some of them have become endangered.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Take the plant Chunlan as an explant, first wash the plant, peel off the outer leaves, cut the plant into 3 cm long, soak it in 75% alcohol for 30 seconds, disinfect it with 0.1% mercury chloride for 5 minutes, and rinse it with sterile water Rinse 4 times; followed by peeling off the terminal buds and lateral buds, and inserting the sterilized terminal buds and lateral buds into the prepared medium, which includes MS basic medium and additional substances with different components, wherein the additional components 6-Benzyl aminopurine (6-Benzyl aminopurine referred to as BA) 2mg / L, naphthaleneacetic acid (I-NaphthyIacetic acid referred to as NAA) 0.5mg / L, agar 7000mg / L, sugar 20000mg / L, coconut milk 10%, banana puree 5% mixed; the third is light culture, the temperature is 20-28 °C, the light intensity is 1500-3000Lx, the light is 10-16 hours a day, and the culture is 30-90 days to make new shoots grow, and then every 30-50 days to multiply once.

[0009] Components ...

Embodiment 2

[0030] Take the plant Chunlan as an explant, first wash the plant, peel off the outer leaves, cut the plant into 5 cm long, soak it in 75% alcohol for 20 seconds, disinfect it with 0.1% mercury chloride for 10 minutes, and wash it with sterile water Rinse 5 times; followed by peeling off the terminal buds and lateral buds, and inserting the sterilized terminal buds and lateral buds into the prepared medium, which includes MS basic medium and additional substances with different components, wherein the additional components BA 6mg / L, NAA 5mg / L, 2,4-dichlorophenoxyacetic acid (2,4-dichlorophenoxy acetic acid referred to as 2,4-D) 2mg / L, agar 7000mg / L, sugar 20000mg / L, coconut Juice 10% mixed; the third is light culture, the temperature is 20-28°C, the light intensity is 1500-3000Lx, the light is 10-16 hours a day, and the culture is 60-90 days to make protocorms grow, and then every 30-50 days Multiply once.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The quick reproduction method of cymbidium goeringii uses plant terminal bud or side bud as explant, which includes the following steps: firstly, cutting the plant to a certain tength, soaking it in 75% alcohol for a certain time, using 0.1% chloro mercury to make sterilization, using sterile water to wash, then bud grafted in prepared culture medium, and making light culture for a certain time every day. Said invention is simple and easy to implement, its operation is convenient, and its reproduction speed is quick.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and more specifically relates to a method for rapidly propagating Chunlan by means of a basic medium and additional different components. Background technique [0002] Chunlan [Cymbidium goeringii (Rchb.f.) Rchb.f.] belongs to Orchidaceae, a subtropical plant, native to China, and is a flower with extremely rich wild resources. However, in order to obtain novel treasures, people are digging indiscriminately at the cost of destroying resources, resulting in extremely serious damage to wild resources, and some have become endangered. [0003] The reproduction of Chunlan generally adopts the division method. Due to the extremely low reproduction coefficient under natural conditions, the annual reproduction coefficient is generally around 1, and the reproduction coefficient of some famous and high-quality varieties does not even reach 1. Therefore, although some famo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
Inventor 杨波龙云铭李洪林
Owner 中国科学院武汉植物研究所