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Test-paper testing method for diosgenin content

A technology of diosgenin and a detection method, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of difficult simultaneous detection of a large number of samples, complicated operation, expensive equipment, etc., and achieves simple and cheap instruments and equipment, high sensitivity, and detection process. Simplified effect

Inactive Publication Date: 2004-11-17
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Wherein gravimetric method and colorimetric method require simple equipment, disadvantage is time-consuming, it is difficult to carry out the simultaneous detection of a large number of samples, and the error is relatively large; High performance liquid chromatography is accurate in measuring result, but also time-consuming, and the required equipment is expensive (market It costs hundreds of thousands to millions of yuan to sell a liquid chromatograph), the operation is complicated, and operators who need special training are beyond the reach of ordinary enterprises or individuals.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0042] Sample Preparation:

[0043] Weigh an appropriate amount of pulverized sample to be tested (0.01g-1g, depending on the sample), add methanol for ultrasonic extraction (if there is no ultrasonic equipment, just let it stand overnight) 3 times, 3-5ml each time, and combine the extraction solution , constant volume, activated carbon decolorization.

[0044] Content detection:

[0045] Drop the mixed solution of diosgenin standard substance and anti-diosgenin and related saponin antibody with gradient concentration on the test paper (incubate at 37°C for 1h), wash with phosphate buffer saline → enzyme-labeled goat anti-rabbit (incubate at 37°C for 1h), phosphate Buffer washing→substrate color development, water washing→scanner scanning, measure gray value and draw standard curve;

[0046] Sample detection: drop the mixture of the sample to be tested and anti-diosgenin and related saponin antibodies on the test paper (incubate at 37°C for 1 hour), wash with phosphate buffe...

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Abstract

The invention discloses a test-paper detecting method of the diosgenin content. It uses one albumen-coupled diosgenin as the antigen to get the anti-diosgenin and the relative soap-glucoside antibody; uses the other as the peridium matter, which is combined with the nitric-acid fiber film to make the test paper used as the spot enzyme linked immune method to detect the diosgenin conttent in the plant body or the relative sample.

Description

technical field [0001] The invention relates to a method for detecting the content of diosgenin in plants, in particular to a method for quantitatively detecting the content of diosgenin in plants by using a test paper detection method (spot enzyme-linked immunoassay). Background technique [0002] Diosgenin is the most important raw material for the synthesis of steroid hormone drugs. Due to the cumbersome and time-consuming steps of conventional methods for detecting diosgenin content in plants, it is an urgent problem to be solved to improve the determination method of diosgenin content in plants. [0003] As far as is known, the conventional methods for detecting the content of diosgenin in plants (usually yam plants) include: [0004] ① Gravimetric method: Weigh two parts of the sample to be tested, chopped and dried in the sun (or dry at 80°C, and measure the moisture separately), each 30g, add 300ml of 2N hydrochloric acid respectively, heat and hydrolyze in a water ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/535G01N33/543
Inventor 李家儒胡江丽何骥
Owner WUHAN UNIV