Method for producing oxide with fermentation process
A technology of oxides and microorganisms, applied in methods using microorganisms, methods based on microorganisms, fermentation, etc., can solve problems such as low microbial growth rate, increased by-product formation, and reduced conversion specificity of substrate compounds
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Embodiment 1
[0017] Inoculate 0.5ml of Gluconobacter oxidans N952 (FERM BP-4580) preserved in liquid nitrogen, a transformant of Gluconobacter oxidans (WO95 / 23220) into a 500ml shaker flask containing 50ml of culture medium containing 0.5 % glucose, 5% sorbitol, 1.5% corn extract, and 0.15% magnesium sulfate, cultivated at 30°C for 24 hours. Part of this culture (17 ml) was transferred to a 30 L fermenter filled with 17 L of sterile medium having the same composition as above, and cultured at 30° C. for 20 hours. 2 L of this seed culture was transferred to a 30 L fermentor containing 17 L of medium (containing 15% sorbitol, 2% corn extract, 0.3% yeast extract, 0.5% magnesium sulfate, and 0.5% calcium carbonate), Incubate at 32°C for 70 hours. During the cultivation process, the pH value of the medium was controlled at 5.5 for the first 24 hours, and then an aqueous sodium hydroxide solution was added to control the pH value of the medium to 6.5 until the fermentation was completed, and th...
Embodiment 2
[0018] Gluconobacter oxidans HS17 [nitrosoguanidine-induced mutation of Gluconobacter oxidans NB6939-pSDH-tufB1 (WO95 / 23220) to enhance the efficiency of conversion of sorbitol to 2-keto-L-gulonic acid] instead of oxidation Gluconobacter N952, the rest repeat the culture conditions of Example 1. From 13 hours after the start of the culture, the addition of glycerol equivalent to 6% of the final medium was started until 72 hours after the start of the culture. In control experiments, glycerol equivalent to 6% of the final medium was added once before the start of culture. The efficiency of converting sorbitol into 2-keto-L-gulonic acid was determined, and the conversion efficiency of the experimental group and the control medium were compared respectively at 24, 48, 56, and 72 hours after the initiation of culture. The results are shown in Table 1.
[0019] 24 hours
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