Tissue chip used for tumour early stage diagnosis and preparation device
A tissue chip, early diagnosis technology, applied in the direction of instruments, analytical materials, etc., can solve the problems of early diagnosis and prediction of malignant tumors that cannot be screened with high throughput
Inactive Publication Date: 2006-04-26
中南大学湘雅医学院肿瘤研究所
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Problems solved by technology
[0003] The present invention aims to develop a new type of tissue chip to solve the problem that the current tissue chip cannot be used for high-throughput screening of molecular markers for early diagnosis and prognosis of malignant tumors
Method used
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Embodiment
[0018] Example: nasopharyngeal carcinoma tissue chip and its fabrication
[0019] (1) Specimen preparation and target tissue HE staining section positioning: 250 paraffin tissue specimens of nasopharyngeal carcinoma, 150 cases of precancerous lesions and 48 cases of corresponding normal nasopharyngeal tissues were selected from the tissue specimen bank with complete clinical and pathological characteristics. , Routine dehydration, paraffin embedding, sectioning, HE staining, confirming the correctness of the tissue under the microscope, and marking and positioning the target tissue in the section under the microscope. (2) Production of recipient paraffin blocks: tissue microarrays with a tissue diameter of 1.0mm, including nasopharyngeal carcinoma, precancerous lesions and normal nasopharyngeal tissues, with a total of 448 specimens, using mold paper with 448 tissue point arrays ( image 3 ) on the surface of the receptor wax block to guide the preparation of the receptor hole...
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The invention relates to a tissue chip. The invention selects paraffin tissue samples, precancerous lesions and corresponding normal tissue samples of common malignant tumors such as lung cancer, nasopharyngeal cancer, esophageal cancer, gastric cancer, colorectal cancer, liver cancer, breast cancer, and cervical cancer with complete clinical data and pathological characteristics. , dyeing, marking and positioning, design dot array mold paper to paste on the surface of the receptor wax block to guide the production of receptor holes, and use simple and practical perforating needles and tissue puncture needles to prepare tissue core paraffin blocks; the present invention includes cancer tissue, cancer Tissue microarray products of pre-lesions and corresponding normal tissues. In situ hybridization and immunohistochemistry were used to detect the expression of related gene mRNA and protein on the tissue chip, which was verified to be consistent with the results of conventional detection, and the cell morphology was clear, uniform, and no tissue points fell off. Each sample in the chip has corresponding clinical and pathological data, which can be used to detect the expression differences of various target genes in large samples and various types of cancer tissues, and provide high-throughput molecular markers for screening early tumor diagnosis and predicting prognosis. measurement tool.
Description
Technical field: [0001] The invention relates to biological products for tissue detection, in particular to a tissue chip. Background technique: [0002] Tissue chip, also known as tissue microarray (Tissue chip, Tissue Microarray TMA,) is that many donor tissues are rearranged on the recipient wax block in the form of a microarray, and the recipient wax block is sliced and transferred to a glass slide. Sections including hundreds of tissues can be used for a variety of in situ expression studies. Tissue microarrays are often used to puncture the tissue of the donor wax block and make the acceptor hole of the recipient wax block. At present, there are tumor tissue chips, normal tissue chips, single or composite tissue chips, etc. on the market, but they cannot be used for high-throughput screening of molecular markers for early diagnosis of malignant tumors and prediction of prognosis. Therefore, it is urgent to make a tissue microarray that can be used to detect the exp...
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IPC IPC(8): G01N35/00
Inventor 李桂源范松青马键李小玲谭琛熊炜黄河周鸣
Owner 中南大学湘雅医学院肿瘤研究所