Method for extracting effective active component of rodgersia aesculifolia
An active ingredient and extraction method technology, which is applied in the field of extraction of effective active ingredients of Sogudan, can solve problems such as lack of effective antiviral means, and achieve the effects of no toxic side effects, obvious curative effect, and enhanced immunity.
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Embodiment 1
[0020] Take 200g of the root tuber of Suogudan, select it, slice it, wash it, dry it, and crush it to a particle size of 10 meshes; use 1000g of medical ethanol with a concentration of 45% to 60% for total extraction, and the extraction method is cold soaking. The time is 7 days each time, the number of extractions is 4 times, and the extraction temperature is 25°C. Then the polar part is extracted with an organic solution, the organic solution can be chloroform, ethyl acetate, n-butanol; then conduct an activity test, cooperate with pharmacological screening, and discard the residue; after the solvent is recovered under reduced pressure, the extract is concentrated into an extract; Parts are chromatographically separated with a chromatographic column, column chromatography uses silica gel as a filler, and uses chloroform: ethyl acetate: formic acid = 12:5:0.1 as an eluent to obtain the eluent of the active part, that is, the effective active ingredient .
Embodiment 2
[0022] Take 150g of the root tuber of Suogudan, select it, slice it, wash it, dry it, and crush it to a particle size of 3-5mm; use 750g of medical ethanol with a concentration of 70-75% for total extraction, and the extraction method is a heat reflux method , The reflux time is 3 hours each time; the reflux times are 3 times. Then the polar part is extracted with an organic solution, the organic solution can be chloroform, ethyl acetate, n-butanol; then conduct an activity test, cooperate with pharmacological screening, and discard the residue; after the solvent is recovered under reduced pressure, the extract is concentrated into an extract; Use chromatographic column for chromatographic separation of parts, column chromatography uses polyamide as filler, and n-butanol: chloroform: formic acid = 8:3:1 as eluent to obtain the eluent of the active part, that is, the effective activity Element.
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