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Method for investigating mechanism of action of traditional Chinese medicine

A technology of action mechanism and determination method, which is applied in the field of biosensing analysis technology for the determination of the action mechanism of traditional Chinese medicines, can solve the problems that the influence of the combination of effective components of traditional Chinese medicines is not detected, and the patent literature reports on the mechanism of action of traditional Chinese medicines are not found. To avoid the influence of subjective factors and achieve the effect of good repeatability

Active Publication Date: 2006-06-14
NANKAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although researchers have used molecular biology methods to detect the changes in the expression of related cytokines (Chen Zhizhong. Biospecific interaction analysis and its application progress. Chinese Journal of New Drugs. 2001; 10(11): 829; Li Hong, Zhou Mou .Research on the regulation of the expression of adhesion molecules by traditional Chinese medicine. Chinese Medical Journal. 2002; 30(1):27-29), but there is no research on the influence of active ingredients of traditional Chinese medicine on the interaction between cytokines and their receptors to report
[0004] After searching the patent literature, there is no patent literature report on the determination of the mechanism of action of traditional Chinese medicine

Method used

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  • Method for investigating mechanism of action of traditional Chinese medicine
  • Method for investigating mechanism of action of traditional Chinese medicine
  • Method for investigating mechanism of action of traditional Chinese medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The selected traditional Chinese medicine is Radix Paeoniae Rubra, which promotes blood circulation and removes blood stasis, and its active ingredient is Chishao 801; the selected cytokines and their receptors are human recombinant cytokine endothelin-1 (ET-1) and human recombinant cytokine Receptor (ET-1 receptor); The selected activated biotin is SULFO-NHS-LC-BIOTIN; The selected avidin is Strepavidin; The selected biosensing system is the SEIKO QCA917 type of EG&G Company, with Winchem software and The surface area is 0.19±0.01×10 -4 m 2 , Thickness 0.3mm, F 0 =9MHz AT-cut gold quartz crystal substrate piezoelectric quartz crystal microbalance.

[0059] In the first step, add 4-100 μg of human recombinant cytokine endothelin-1 into 200-500 μL of phosphate buffer solution with a concentration of 0.01mol / L and pH=7.4; activate biotin with 1-2 mg of SULFO-NHS-LC-BIOTIN Dissolve in 120 μL of deionized water, take 20-50 μL of it and add it to the above-mentioned human...

Embodiment 2

[0075] Except that the traditional Chinese medicine Chishao 801 used is replaced by Ligustrazine, the active ingredient of Ligusticum Chuanxiong, the others are the same as in Example 1. The test results of this experiment show that Ligustrazine can specifically interact with human recombinant cytokine endothelin-1 and its receptor. Sexual combination, suggesting that the target of ligustrazine in the body is human recombinant cytokine endothelin-1 and its receptor pathway in addition to the reported thromboxane A2. It inhibits the combination of human recombinant cytokine endothelin-1 and human recombinant cytokine endothelin-1 receptor, thereby antagonizing the effect of endothelin, dilating blood vessels and inhibiting platelet aggregation.

Embodiment 3

[0077] Except that the traditional Chinese medicine Radix Paeoniae Rubra 801 is replaced by ferulic acid, the active ingredient of Chinese medicine Angelica sinensis, the rest are the same as in Example 1. The test results of this experiment show that ferulic acid can interact with human recombinant cytokine endothelin-1 and its receptor The specific binding occurs, suggesting that the target of ferulic acid in vivo is not only the reported thromboxane A2, but also human recombinant cytokine endothelin-1 and its receptor pathway, that is, ferulic acid binds to human recombinant cytokine The combination of endothelin-1 and its receptor inhibits the combination of human recombinant cytokine endothelin-1 and human recombinant cytokine endothelin-1 receptor, thereby antagonizing the effect of endothelin, dilating blood vessels and inhibiting platelet aggregation.

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Abstract

The technical scheme of the present invention relates to a method for determining the mechanism of action of traditional Chinese medicine by using biosensing analysis technology, and using a piezoelectric quartz crystal microbalance to detect the interaction between the active ingredients of Chinese medicine and cytokines, the interaction with cytokine receptors, and the effect on cells. Factors and their receptors interact with each other, analyze the target and mechanism of action of the active ingredients of traditional Chinese medicine, and clarify the mechanism of action of traditional Chinese medicine, and can thus perform screening and compatibility of traditional Chinese medicine. The method of the present invention has real-time, high-efficiency Advantages of sex, sensitivity, specificity, simplicity, and objectivity.

Description

technical field [0001] The technical scheme of the invention relates to the application of biosensing technology to testing or analyzing materials, specifically to the method for measuring the mechanism of action of traditional Chinese medicine by applying biosensing analysis technology. Background technique [0002] In the process of modern research on traditional Chinese medicine for nearly half a century, there have been many studies on the clinical efficacy and pharmacology of traditional Chinese medicine, whether it is a single medicine or a combination of compound prescriptions. However, due to the lack of suitable research methods and means It has not been studied in depth, and this has become one of the bottlenecks in the further research and development of Chinese medicine. In recent years, with the development of instrumental analysis technology, such as ESI / APCI-MS, NMR and the enhancement of interdisciplinary research on Chinese medicine, some new methods and the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/15G01N33/50G01N33/53C12Q1/00
Inventor 陈强陈可冀吴宝艳殷惠军黄加栋李静史海滨
Owner NANKAI UNIV