Agarobiose-contg. composition
A technology of agarobiose and composition, applied in the field of agarobiose-containing composition, to achieve the effects of improving quality and yield, reducing cost, and increasing substrate concentration
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Embodiment 1
[0091] To prepare the agarobiose-containing composition, a commercially available agar (Ina agar S-7 type, Ina Shokuhin Kogyo) was dissolved in deionized water at a concentration of 10% (w / v). Then add the activated form (H + ) strong cation exchange resin (Diaion SK-104, Mitsubishi Chemical). Hydrolysis was carried out at 90°C for 3 hours. After the reaction, the mixture was cooled to normal temperature and subjected to solid-liquid separation (removal of the resin from the solution). The resulting solution was treated with activated carbon at a concentration of 2% (w / v) to remove colored substances and the like and filtered with a filter having a pore size of 1 μm. After adjusting the pH with 1N NaOH, the filtrate was lyophilized according to a conventional method to prepare agarobiose-containing compositional form of Agabiose.
[0092] The Agabiose contains 2.3% water, 9.8% galactose, 44.1% agarobiose, and 43.4% agarooligosaccharides including agarotetraose and agarohexa...
Embodiment 2
[0094] Dissolve S-7 type Ina agar in deionized water at a concentration of 10% (w / v). Add the activated form (H + ) strong cation exchange resin. Hydrolysis is carried out at 90°C for about 1.5 hours. After the reaction, the mixture was cooled to normal temperature and subjected to solid-liquid separation. The resulting solution was treated as described in Example 1 to prepare agarobiose-containing compositional form of Agaoligo.
[0095] The Agaoligo contains 2.3% water, 9.5% galactose, 16.2% agarobiose, and 70.3% agarooligosaccharides including agarotetraose and agarohexaose (pH 5.1).
Embodiment 3
[0097] Dissolve S-7 type Ina agar in deionized water at a concentration of 10% (w / v). To this was added citric acid at a concentration of 1% (w / v). Hydrolysis was carried out at 95°C for 3 hours. After the reaction, the mixture was cooled to normal temperature. The resulting solution was treated with activated carbon at a concentration of 2% (w / v) to remove colored matter and filtered to prepare a citric acid degradation product as an agarobiose-containing composition.
[0098] The citric acid degradation product contains 10% solids, 1% (w / v) citric acid, 3.1 g / L galactose, 14.8 g / L agarobiose, and 80 g of agarose oligosaccharides including agarotetraose and agarohexaose / L (pH 2.6).
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