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Method for quick breeding of wild water-chestnut

An engineering and wildling technology, applied in horticultural methods, botanical equipment and methods, horticultural tools/equipment, etc., can solve problems such as transplantation habitat pollution, origin habitat destruction, physiological and ecological test errors, etc., to avoid genetic variation, Simple technology and convenient operation

Inactive Publication Date: 2007-08-15
NANJING UNIV
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  • Summary
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Problems solved by technology

At present, there is a great demand for wild water chestnut seedlings in the market, which are used in experiments and ecological engineering, but the source of the seeds comes from the natural environment mostly, and a large number of salvages can easily lead to the destruction of the original habitat; although wild water chestnut seeds can be planted to make up, but planting not only requires a suitable aquatic environment and a long time, but also is limited by its own low seed germination rate
In addition, wild water chestnuts in natural habitats often contain various algae and miscellaneous bacteria, which may easily cause pollution of transplanted habitats and errors in related physiological and ecological experiments.
[0004] So far, there is no research and report on the artificial propagation of aseptic seedlings of wild water chestnut at home and abroad

Method used

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Embodiment Construction

[0024] The provenance of wild water chestnut was collected from Lishui, Nanjing in December 2004, and 30 germs were dissected, soaked in soapy water for 2 hours, washed with tap water, and rinsed under running water overnight. In the ultra-clean bench, with 75% alcohol and AgNO 3 After a certain period of thorough sterilization, wash with sterile water and inoculate in culture medium ①. They were cultivated in a tissue culture room with an average light intensity of 2000 lx, 10 hours of light per day, and a temperature of 25°C. Within 2 weeks, 4 buds of the removed infected bacteria and dead materials were removed, and the remaining 26 buds could all grow. Healthy seedlings were transferred to the medium ②, and continued to be cultivated in the tissue culture room, and the wild water chestnut began to grow strong, and bud proliferation appeared. Then transfer to the culture medium ③, the buds of the wild water chestnut proliferate in large quantities, and new individuals gro...

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Abstract

A method for fastly reproducing the high the high-quality engineered seedlings of wild water caltrop features use of tissue technique for reproduction.

Description

1. Technical field [0001] The invention belongs to the field of biotechnology and relates to a method for rapidly propagating high-quality engineering seedlings of wild water chestnut. 2. Background technology [0002] Wild water chestnut (Trapa incisa) is an annual floating-leaf aquatic herb belonging to the genus Trapa incisa. It likes temperature, water and sufficient sunlight, and has a long growing season. The bottom is soft and fertile, the humus content is high, and the ponds, ditches and lakes with thick silt deposits are suitable for planting. This species has a high tolerance to water pollution and can be used to purify water in aquatic ecosystem restoration and reconstruction projects; this species also contains more nutrients and can be used as fruits, vegetables, and grains; and has a relatively high High medicinal value. [0003] Wild water chestnut has been listed as a second-class national key protected wild plant, and the protection of wild water chestnut...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/00
Inventor 安树青蔡颖关保华王秉萱
Owner NANJING UNIV
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