Protein chip for diagnosing autoimmune diseases
An autoimmunity and protein chip technology, applied in the direction of biological testing, material inspection products, etc., can solve the problems of cumbersome tissue section operations, increased risk of results, and poor sensitivity of direct methods, so as to facilitate early diagnosis, stable test results, Practical effect
Inactive Publication Date: 2002-03-06
GENETECH BIOTECH SHANGHAI
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Problems solved by technology
[0004] Immunocytochemistry is a commonly used method for diagnosing autoimmune diseases. There are certain defects: first, the tissue section operation is cumbersome, time-consuming and laborious, especially the direct method, which requires tissue biopsy of the patient, which is more harmful; second, the direct method is sensitive It is poor, and one fluorescent antigen can only detect one antibody, especially for non-organ-specific autoimmune diseases, which requires a comprehensive judgment of multiple indicators; while the indirect method has slightly improved sensitivity, but the operation is cumbersome and the specificity is not good. high
Third, since these two methods rely on the observation of the tissue under the microscope by the inspector, the technical requirements are extremely high, which increases the risk of the result
Fourth, with traditional technology, only one patient can be tested on one slide, and the detection indicators are limited
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[0019] The protein chip for the combined diagnosis of autoimmune diseases of the present invention includes all known autoantibodies in the detection indicators of autoimmune diseases, mainly including: antinuclear antibodies, rheumatoid factor (RF), anti-acetylcholine receptor (AchR) antibodies, Anti-blood cell antibodies, anti-thyroglobulin and microsomal antibodies, etc., the antigens arrayed on the solid-phase carrier are characteristic antigens that can specifically bind to the above-mentioned antibodies. The protein samples are dotted on the solid-phase carrier, and the dotted array of the protein samples is designed on a multi-sample microarray biochip. The protein samples refer to the antigens of various indicators of autoimmune diseases, as well as the positive control and the negative control. In the same chamber of the multi-sample microarray biochip, each chamber of the multi-sample microarray biochip is dotted with the antigens of the above multiple indicators. Th...
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A protein chip for diagnosing autoimmune diseases is prepared through arraying the antigens corresponent to the different indexes of different autoimmune diseases, positive references and negative references on solid carrier by high density, and fixing the protein to said solid carrier by chemical bonds. It is applied by arraying the protein specimens in a same cavity of said micro-array biochip to obtain the reaction results for different indexes by only one reaction. Its advantages include high sensitivity and specifity, high stability and reliability of result, less damage to human body and low cost.
Description
technical field [0001] The protein chip for combined diagnosis of autoimmune diseases of the invention belongs to the field of life sciences. The invention relates to a protein chip, a preparation method and a detection method. The protein chip is a protein or polypeptide array arranged in an orderly manner on a solid substrate, so as to realize parallel detection, identification, identification and diagnosis of proteins. It can be widely used in biology, medicine and related fields. Background technique [0002] Autoimmune diseases refer to diseases caused by the body's immune response to self-antigens, resulting in damage to its own tissues. Common autoimmune diseases can be divided into non-organ specific and organ specific. Systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) belong to the former. etc. belong to the latter. The initial cause and exact pathogenesis of autoimmune diseases are not clear, and the course of the disease is generally long, mostly...
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Login to View More IPC IPC(8): G01N33/68G01N33/96
Inventor 张涛李宾彭永济陈莹任一萍
Owner GENETECH BIOTECH SHANGHAI