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Method for raising Resveratrol content in Chinese herb-giant knotweed rhizome

A technology for resveratrol and Polygonum cuspidatum, which is applied in the field of extracting medicinal raw materials, can solve the problems of high cost, complicated process, long enzymatic hydrolysis time and the like, and achieves the effects of easy operation, extensive resources and low extraction cost.

Inactive Publication Date: 2002-12-18
深圳清大现代中医药科技有限公司
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Problems solved by technology

However, the process of extracting resveratrol by enzymatic hydrolysis is more complicated. It needs to crush Polygonum cuspidatum into powder before enzymatic hydrolysis. The enzymatic hydrolysis takes a long time and the cost is high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0015] 1. Fermentation

[0016] Take 1 kg of knotweed and put it in a container, add water to cover the medicinal surface, control the temperature at 35-45°C, soak and ferment for 72 hours, filter, discard the water, and add ethanol directly to extract resveratrol from the knotweed with water or dry it in the sun spare;

[0017] 2. Extraction

[0018] Take 1 kg of fermented Polygonum cuspidatum, add 5000 ml of 70% ethanol to reflux and extract for 3 hours, filter, and keep the filtrate. The dregs were extracted with 3000ml of 70% ethanol for 1.5 hours, and then filtered. The two ethanol extracts were combined and concentrated under reduced pressure until the raffinate had no alcohol smell. Add water to the residue to 500ml, transfer to a 1000ml separatory funnel, add 300ml and 200ml of ethyl acetate, shake and extract twice. Combine the ethyl acetate extracts, wash with 1% sodium carbonate solution three times, each time with 100ml of sodium carbonate solution, then wash w...

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Abstract

The technological process for raising Resveratrol content in the Chinese medicinal materials bushy knotweed root includes the following steps: soaking busky knotweed root in water, then placing it incontainer whose temp. is controlled at 20-50 deg.C, fermenting for 24-96 hr, to obtain fermented bushy knotweed root, then extracting by using solvent and concentrating so as to obtain the resveratrol semi-finished product. Said invention is simple in process, and high in resveratrol conversion rate, and low in cost.

Description

technical field [0001] The method for extracting medicinal raw materials from Chinese medicinal materials involved in the present invention is especially a method for increasing the content of resveratrol in Chinese medicinal Polygonum cuspidatum. technical background [0002] Resveratrol (Resveratrol), chemical name stilbene (3,5,4-trihydroxystilbene), has obvious antibacterial, anti-inflammatory, anti-cancer, anti-thrombosis, anti-hyperlipidemia, anti-lipid side oxidation and many other aspects Its pharmacological activity is widely used in food, health products, cosmetics and other fields. Since veratrol is widely present in plants such as grape family, encyclopedia, leguminous family, umbelliferae, sedge family, palm family, and vine family. Studies have found that currently resveratrol has the highest content in Polygonum cuspidatum, and Polygonum cuspidatum also contains resveratrol glucoside (polydatin). Chinese invention patent application 99115156.9 discloses "A P...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/22
Inventor 邹贤德舒鑫
Owner 深圳清大现代中医药科技有限公司
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