Method of utilizing biosynthesis and chemical modification technology in producing thymic hormone al monomer

A chemical modification and biosynthesis technology, applied in the fields of biosynthesis and chemical modification, can solve the problems of high cost of thymosin α1, many toxic reagents, and difficulty in large-scale implementation, and achieve the convenience of large-scale production, low production cost, and high technology simple effect

Inactive Publication Date: 2003-01-01
吴建中
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  • Abstract
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Problems solved by technology

However, the cost of preparing thymosin α1 by chemical methods is high, and it

Method used

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Embodiment Construction

[0025] The present invention will be further described below in conjunction with specific implementation methods and experimental results. Example: the method for producing thymosin α1 monomer for the expression of three-molecule or two-molecule linked thymosin α1 in Escherichia coli and its chemical cleavage and modification technology.

[0026] The present embodiment method comprises the following steps:

[0027] 1. Using artificial gene synthesis technology to obtain the complete sequence of the thymosin α1 gene; specifically including:

[0028] 1) Design a thymosin α1 gene sequence fragment and synthesize it using an automatic DNA synthesizer. The synthesized fragment is as follows: Fragment 1: AAT ATG TCT GAT GCT GCT GTT GAT ACT TCT TCT GAG ATT ACT ACTAAA GAT CTT AAG GAG AAG AAG GAA GTT GTC GAA GAG GCT GAG (87mer) Fragment 2: CAT ATT CTC AGC CTC TTC GAC AAC TTC CTT CTT CTC CTT AAG ATCTTT AGT AGT AAT CTC AGA AGA AGT ATC AAC AGC AGC ATC AGA (87mer) Fragment 3: AGT AGT AAT ...

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Abstract

The present invention belongs to the field of biosynthesis and chemical modification technology. By means of bioengineering process, several thymosin genes are recombined and the recombinant gene is expressed in host cell to obtain high-expression thymosin fusion body. The fusion body is cracked and modified chemically to form thymosin alpha 1 monomer finally. The production process can produce thymosin alpha 1 identical with that produced chemically and is simple, low in cost and suitable for production in large scale. The present invention also provides the production process of several thymosin alpha 1 derivatives.

Description

technical field [0001] The invention belongs to the technical field of biosynthesis and chemical modification, and particularly relates to a production method of thymosin α1 for improving human immunity and treating various diseases. Background technique [0002] Thymosin α1 was discovered as early as 1977. It is a 28 amino acid acidic small molecular polypeptide, which was originally isolated from thymus. There have been extensive and in-depth studies on its physiological effects. Whether it is in vivo or in vitro analysis, it shows a strong stimulating effect on the immune system of the body, promotes the maturation of T lymphocytes, and is an immune enhancement factor of T lymphocytes. Clinically, it can be applied to the adjuvant treatment of many diseases related to the immune system, such as hepatitis B, hepatitis C, cancer, immunodeficiency, low immunity, and AIDS. [0003] At present, the pure product of thymosin α1 is a chemical drug, which has been listed both at...

Claims

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Application Information

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IPC IPC(8): C07K14/575
Inventor 吴建中
Owner 吴建中
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