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Method for extracting tea polysaccharide from tailings with tea polyphenols being extracted

A technology of leftovers and tea polysaccharides, which is applied in the field of extracting tea polysaccharides, can solve the problems of low extraction yield, energy and alcohol consumption, and increased production costs, and achieve the effects of simple process, saving production costs, and improving utilization

Inactive Publication Date: 2003-07-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional method is used to extract tea polysaccharides. Although the source of raw materials is rich, the extraction rate is low, because alcohol precipitation only works on macromolecular polysaccharides, and there are still a large number of small molecule oligopolysaccharides with medicinal effects that have not been precipitated. In solution
At the same time, it consumes a lot of energy and alcohol, which increases the production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Take 300 grams of leftover powder and dissolve it in warm water of about 50 degrees. The volume of water is 2100ml. After dissolving, put the sugar liquid into a water bath at a temperature of about 40 degrees, add 1% pectinase to act for 2 hours, then centrifuge at a speed of about 3000 rpm to remove insoluble substances, and then add 2 times the volume of chloroform to the remaining liquid to stir and extract , after standing still for 30 minutes, release the chloroform layer at the bottom, and dry the remaining transparent and clear brown solution with a vacuum dryer to obtain tea polysaccharides and oligomeric polysaccharides.

Embodiment 2

[0018] Get 500 grams of leftover powder, dissolve with 55 degree warm water, the volume of water is 4500ml. After dissolving, put the sugar liquid in a water bath at a temperature of about 50 degrees, add 5% pectinase to act for 2.5 hours, then centrifuge at a speed of about 3500 rpm to remove insoluble substances, and then add 5 times the volume of chloroform to the remaining liquid to stir and extract After standing for 30 minutes, the chloroform layer at the bottom is released, and the remaining transparent and clear brown solution is dried with a vacuum dryer to obtain tea polysaccharides and oligopolysaccharides.

Embodiment 3

[0020] Get 800 grams of scrap powder and dissolve it in 55 degree warm water, the volume of water is 8000ml. After dissolving, put the sugar liquid into a water bath at a temperature of about 60 degrees, add 10% pectinase to act for 3 hours, then centrifuge at a speed of about 4000 rpm to remove insoluble substances, and then add 8 times the volume of chloroform to the remaining liquid to stir and extract After standing for 30 minutes, the chloroform layer at the bottom is released, and the remaining transparent and clear brown solution is dried with a vacuum dryer to obtain tea polysaccharides and oligopolysaccharides.

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PUM

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Abstract

A process for extracting tea polyase from the leftover generated by extracting tea polyphenol includes extracting toxic theocin and most of protein by chloroform and drying. Its advantage is high extracting rate (40% or more).

Description

technical field [0001] The invention relates to a method for extracting tea polysaccharides from leftovers of extracted tea polyphenols. Background technique [0002] Tea polysaccharide is a natural polymer compound composed of glucose, arabinose, ribose xylose, galactose, fucose and other monosaccharides, with an average molecular weight of about 4×10 4 Dalton, tea polysaccharides should also include a variety of small molecule oligosaccharides. Tea polysaccharides have the functions of lowering blood sugar, lowering blood fat, enhancing immunity, anti-atherosclerosis, anti-coagulation, and anti-thrombosis. Although there are various techniques for extracting tea polysaccharides from tea leaves, most of them have the following main steps: [0003] Tea powder—→dissolve in hot water—→centrifuge to remove insoluble impurities—→obtain polysaccharide-containing supernatant—→use Sevag method or other methods to remove protein—→add 3-5 times the volume ...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 李永泉吴丹姚恕
Owner ZHEJIANG UNIV
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