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Lentinan molecular weight and molecular weight distribution measuring method

A technology for molecular weight distribution and lentinan, which is applied in measurement devices, material separation, and analysis of materials, etc., can solve the problems of actual molecular weight error and different chemical structures, and achieve the effect of improved accuracy and small error.

Inactive Publication Date: 2003-08-20
NANJING LUYE PHARMA
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Problems solved by technology

However, the current method for determining the molecular weight of macromolecular polysaccharides uses a standard substance that is often different from the chemical structure of the substance to be tested, and thus establishes a relative standard curve, and the measured result is a relative molecular weight, which is related to the actual molecular weight of the substance to be tested. large error

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  • Lentinan molecular weight and molecular weight distribution measuring method
  • Lentinan molecular weight and molecular weight distribution measuring method
  • Lentinan molecular weight and molecular weight distribution measuring method

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Embodiment Construction

[0040] The implementation steps of the present invention are to adopt commercially available standard substances Pullulan series or dextran (Dextran) series to respectively measure relative standard curves and calculate K and α values.

[0041] The present invention further adopts Lentinan fractionated product to measure its intrinsic viscosity, and uses light scattering method to measure the molecular weight of its fractionated product, establishes a universal calibration curve, and establishes a general-purpose high-efficiency gel permeation chromatography with Dextran or Pullulan as a standard ( HPGPC) is a method for measuring the molecular weight of lentinan and its distribution.

[0042] The present invention is based on the experimental research data of twenty-five batches of Lentinan (trade name: Tiandixin, the same below) molecular weight and molecular weight distribution (distribution for short, the same below). See Table 2K, the data statistics table of α value.

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Abstract

The commonly applicable method of measuring the molecular weight and molecular weight distribution of natural macromolecular lentinan features the high performance gel permeation chromatography (HPGPC) is used, with flowing phase superpure water, 0.1-0.5 M solution nitrate, or 0.1-0.5 M sodium acetate and phosphate buffing solution; 1-4 proper chromatographic column of separation range 1-2000 KD and at 30-55 deg.c as analyzing columns, the flow rate of 0.1-1.0 ml / min; standard matter Dextran or Pollulan for drawing relative standard HPGPC curve; and the standard matter graded lentinan products for drawing HPGPC calibration curve.

Description

technical field [0001] The invention relates to a method for measuring molecular weight and molecular weight distribution, in particular to a method for measuring molecular weight and molecular weight distribution of lentinan. Background technique [0002] Lentinan is a Biological Response Modifier (BRM) widely used in anti-tumor chemotherapy and radiotherapy. Especially when applied to gastric cancer, lung cancer, intestinal cancer, and breast cancer, it has obvious chemotherapy synergies and reduces the toxic and side effects of chemotherapy and radiotherapy. Lentinan is a polydisperse polymer with β-D-(1→3) glucose as the main chain and β-D-(1→6) glucose as the side chain. Studies have found that the molecular weight of lentinan has a correlation with animal and even clinical anti-tumor activity. Therefore, it is of great significance to study the determination method of the molecular weight of lentinan to control the intrinsic quality of lentinan. However, the current ...

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Application Information

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IPC IPC(8): G01N21/3577G01N24/08G01N30/02G01N30/36G01N30/46
Inventor 程光程培元张伟东方贻功
Owner NANJING LUYE PHARMA
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