Method for producing callus and differentiating sprout by inducing cistanche salsa seed

Abstract

The process of producing callus and differentiating sprout by inducing cistanche salsa seed includes the following steps: inoculating cistanche salsa seed through heat treatment and sterilization to MS cultivation medium of 2,4-D and/or KT for inducing cultivation; and transferring the callus of cistanche salsa produced in the inducing cultivation solid cultivation medium with B5 cytokinin of 6-BA and/or GA3 for secondary culture. The heat treatment activates the seed of cistanche salsa and the synergistic action of 2,4-D and/or KT overcomes the independence of the seed of cistanche salsa on the host in sprouting, so that the present invention can raise the incidence rate of the callus of cistanche salsa seed, promote cell division and induce tissue differentiation and differentiate sprout of callus.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for inducing callus and differentiation and budding of Cistanche seeds. Background technique [0002] 80% of traditional Chinese medicinal materials are wild resources. Due to the deterioration of the ecological environment and people's blind collection of wild resources, the way of extracting secondary metabolites from natural wild plants has been challenged. Producing secondary metabolites through plant tissue and cell culture is a better way to solve this problem. At present, comfrey, ginseng root, Artemisia annua, foxglove, etc. have reached pilot scale and industrial scale (Curtin M E, Biotechnology , 1983, 1, 649-657). Through callus differentiation and budding, meristem culture and rapid vegetative propagation can provide sufficient seedlings for plant growth and reproduction. At present, rapid propagation technology has been us...

AI Technical Summary

Problems solved by technology

In the natural environment, the germination and growth of Cistanche deserticola must have the existence of hosts, so it is limited by natural conditions, and the cultivation period is long (3-5 years), and artificial cultivation is difficult, so artificial cultivation has certain limitations (Zheng Xingguo et al. , Xinjiang Forestry, 2001, 2, 29-30); At present, there are reports that hormones are used to treat Cistanche seeds to increase the incidence of callus in Cistanche seeds, but generally speaking, if only hormones are used, the occurrence of callus in Cistanche seeds The frequency is low, only 12% (Wu Xin et al., Northwest Pharmaceutical Journal, 1998, 13, 103-104)

[0005] In the callus induction culture of Cistanche seeds, the traditional Chinese medicine overcomes the dependence of Cistanche on the host to make it germinate. According to the new research, there is no report of treating the host plant seeds with high temperature to make the callus.