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Method for testing virus removing effect with air purifier

An air purification device and virus technology, applied in the field of aerobiology and air purification, can solve the problems of complicated operation, long measurement period, insufficient detection sensitivity, etc.

Inactive Publication Date: 2004-02-04
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

2) Whether the virus of humans and animals has been inactivated can only be determined by cell culture method, so the determination cycle is longer and the detection sensitivity is not enough
[0003] After literature search, it is found that the applicant is: Institute of Molecular Biology of Bu Angladi Company. The name of the Chinese invention patent is: phage detection method for biomolecules in biological samples, and the patent number is ZL98806434.0. This patent discloses a The method of using phage to detect the presence of target molecules in biological samples is limited to the use of special devices in the laboratory. The operation is complicated and the detection time is long. It needs to rely on the connection between the molecule and the phage's own genome to detect the presence of the target molecule.
This method has nothing to do with the determination of phage activity in the air, and cannot be used to evaluate the effect of virus clearance in the air

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: Evaluate the virus removal effect of "electrostatic precipitator and ultraviolet disinfection combined treatment device" (hereinafter referred to as device) with bacteriophage mist.

[0038] Install the device in the processing section of the system. Add concentration of 10 to the ultrasonic humidifier 6 pfu / ml of liquid containing phage. Place a gelatin + LB double-layer board with a diameter of 9 cm at the air outlet of the device facing the wind direction. The system runs at a wind speed of 2.5 m / s. Start the ultrasonic humidifier to generate mist. After 1 minute, turn off the humidifier and fan. The gelatin plates were removed and recovered to determine the number of phage captured on each plate. The system was blown again for 5 minutes, and then started the next measurement. Measure 5 times when the device is charged and uncharged. The experiment was repeated 3 times. The phage captured per plate was 90.2±35.79pfu when the device was not charged,...

Embodiment 2

[0039] Embodiment 2: Evaluate the virus removal effect of the "photocatalytic disinfection and sterilization composite treatment device" (hereinafter referred to as the device) with bacteriophage mist.

[0040] Install the device in the processing section of the system. Add concentration of 10 to the ultrasonic humidifier 7 pfu / ml of liquid containing phage. Place a gelatin + LB double-layer board with a diameter of 9 cm facing the wind at the air outlet of the device. The system runs at a wind speed of 5 m / s. Start the ultrasonic humidifier to generate mist. After 1 minute, turn off the humidifier and fan. The gelatin plates were removed and recovered to determine the number of phage captured on each plate. The system was blown again for 10 minutes, and then started the next measurement. Replace the device with an air duct of the same shape and size as the device, and repeat the measurement with the same parameters and working conditions as mentioned above, as a control. ...

Embodiment 3

[0041] Embodiment 3: Evaluate the virus removal effect of "silver-plated filter net antivirus and sterilization composite treatment device" (hereinafter referred to as device) with bacteriophage mist.

[0042] Install the device in the processing section of the system. Add concentration of 10 to the ultrasonic humidifier 7 pfu / ml of liquid containing phage. Place a gelatin + LB double-layer plate with a diameter of 9 cm at the air outlet of the device facing the wind direction. The system runs at a wind speed of 8 m / s. Start the ultrasonic humidifier to generate mist. After 1 minute, turn off the humidifier and fan. The gelatin plates were removed and recovered to determine the number of phage captured on each plate. The system was blown again for 10 minutes, and then started the next measurement. Replace the device with an air duct of the same shape and size as the device, and repeat the measurement with the same parameters and working conditions as mentioned above, as a c...

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Abstract

A method for detecting the effect of air cleaning apparatus in removing virus features that a test platform with multi-cleaning and-disinfecting system for centralized air conditioner is used, the bacteriophile liquid drips or dust are blown through the air cleaning apparatus to be tested, and the number of bacteriophiles at its entrance and the number of bacteriophiles at its exit are measured to reflect its effect. Its advantages are high sensitivity, and high efficiency.

Description

technical field [0001] The invention relates to a method for measuring the virus-removing effect of an air purification device, in particular a method for measuring the virus-removing effect of an air-purifying device by using bacteriophage to simulate viruses in the air of a central air-conditioning system, and belongs to the fields of aerobiology and air purification. Background technique [0002] The outbreak of the SARS virus has caused the public to have unusual concerns and worries about the biological quality of indoor air. How to remove various respiratory viruses including SARS virus in the air has become an urgent problem to be solved at present. Airborne microorganisms exist in the form of bioaerosols. Bioaerosols of respiratory pathogens are typically formed by coughing, loud speaking and sneezing by sick people. In addition, viruses in excrement can be released into the air in the form of aerosols through the splash and swirl caused by flushing the toilet. Ai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06F24F3/16
CPCY02A50/20
Inventor 赵立平徐学敏徐宇虹胡钧王如竹晋欣桥谷波陈亚珠陈杰毛军发林志新胡文彬上官文峰郑晓红
Owner SHANGHAI JIAO TONG UNIV
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