Gene PSME3 of fat thickness at back of pig as well as preparation method

Abstract

The present invention relates to the cloning and preparing process of the pig gene P3ME3 associated with thickness of back fat. Its cDNA and DNA sequences are cloned. The base mutation at three sites are detected, One of them is associated with the thickness of back fat. A process for preparing said gene and the primer sequence for detecting the base mutation at three sites are also disclosed.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to the cloning and preparation method of pig backfat thickness PSME3 gene. Background technique [0002] With the continuous growth of people's demand for animal protein food and the development of commodity economy, carcass quality evaluation has become a common concern of geneticists, breeders, producers, meat dealers and consumers. With the development of domestic and foreign live pig and pork product commodity markets and the improvement of scientific and technological level, the standardization and standardization of carcass quality evaluation have become research hotspots. The currently recognized carcass quality evaluation indicators in the world include: carcass lean meat Lean percentage in the carcass (CLP), lean content in the carcass (CLC) or marketable lean cuts ratio (lean cuts ratio, LCR). In practical work, ...

AI Technical Summary

Problems solved by technology

[0007] The function of the PA28-γ subunit is still not very clear. Murata et al. (Murata S et al., Growth retardation in micelacking the proteasome activator PA28γ: J Biol Chem: 1999, 274: 38211-38215:) used homologous recombination to obtain the deletion of PSME3 Homozygous and heterozygous mice for the region of exons 2 to 8 of the gene, compared with normal mice, these mice were born with normal appearance in all tissues studied, but postnatal growth was retarded, indicating that The function of PSME3 gene may be related to the regulation of cell proliferation and body growth in mice

[0008] Although some important progress has been made in the research on candidate genes of pig backfat thickness, there are still some deficiencies: (1) The important economic traits of pigs are usually quantitative traits, and the physiological and biochemical processes involved are quite complicated. Although 1-2 of its controlled genes have been revealed, there are still other novel genes with large effects to be discovered

(2) The current research on quantitative trait genes basically selects a single candidate gene for analysis, ignoring the interaction between genes

One of the contents of modern molecular breeding is genome breeding, which is based on the overall or whole genome selection, mating, species conservation and heterosis analysis and utilization based on the organizational structure and functional effects of the genes and genotypes of all traits of the individual. A complete high-density gene map, and a full understanding of the organizational structure of all genes, the mechanism of functional expression regulation, and the association with traits. However, the genes and markers that have been genetically and physically mapped in pigs are still very limited. Work in this area still needs to be further strengthened

(3) The method for finding genes with important physiological functions is not comprehensive enough, the number of detected genes is limited, the efficiency is not high, and innovation is needed, and further work to find new genes related to important economic traits of pigs is imminent

[0009] However, the research on porcine PSME3 gene at home and abroad is still blank

Images