Asexual cultivation method of female wood frog
A breeding method, single-sex technology, applied in animal husbandry and other fields
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Embodiment 1
[0022] Select Rana fertilized egg embryos to start feeding tadpole feed bait containing estrogen between the ages of 7 and 30, because at this stage Rana tadpoles deal with the early stage of sexual differentiation, fertilized egg embryos have self-renewal, high-level cultivation, and cell groups have not yet differentiated , and the tadpoles are small, each 0.4 grams, less feeding. It not only saves medicine, but also is easy to mutate into a female wood frog. Rana tadpole feed is made of wheat bran, rice bran, soybean powder, yellow powder, fly maggot pulp, etc. Take 150 mg of estrogen powder and dissolve it in 50 ml of 95% alcohol, mix it evenly into 5000 g of tadpole feed, and dry it in the shade for later use . Feeding method. Take tadpoles aged 7-20 days as an example. At this time, the weight of tadpoles is about 0.5 grams per tadpole, which is 5000 grams per 10,000 tadpoles. Calculated by throwing 4% of the feed, that is, 200 grams of bait is fed every day. 2-3 vot...
Embodiment 2
[0024] The tadpole breeding pool between the ages of 7 and 30 is mixed with estrogen solution at a ratio of 0.5 mg / 1000 ml. From the age of 7 to the age of 30, it is best to filter the water in the tadpole pool once a day to keep the water meter clean.
[0025] And there is sufficient oxygen, or the two pools are used alternately, and the temperature is controlled at 12°C to meet the nutrition of tadpoles. This method is simple, but a waste of drugs.
[0026] In addition, the Rana unisexual female frog breeding method uses estrogen at a concentration of 0.5-mg estrogen per liter of water. From the tail bud stage to the operculum stage, it is sprayed or sprinkled on the feed in the breeding pond. Hatched tadpoles.
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