17 results about "Rana sylvatica" patented technology

This invention relates to molecular marker 1769 and its application in the identification of sex-reversed pseudomale individuals in the Northeast Forest Frog (Rana davidii), and pertains to the field of molecular markers. To address the current difficulty in identifying sex-reversed pseudomale individuals in the Northeast Forest Frog, this invention extracts the genome from the muscle tissue of the left hind limb of the Northeast Forest Frog. Using the extracted genomic DNA as a template, PCR amplification is performed using primer pairs containing the sequences shown in SEQ ID NO:1 and SEQ ID NO:2. The PCR amplification products are detected by electrophoresis on a 3% agarose gel. Phenotypic male individuals exhibiting the 1769 bp band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are genetically male Northeast Forest Frogs, i.e., true males. Phenotypic male individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are pseudomale individuals who have undergone sex reversal from genetically female to phenotypically male. Phenotypic female individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are female individuals.

This invention relates to the molecular marker M-513 and its application in the identification of sex-reversed pseudomale individuals in the Northeast Forest Frog (Rana davidii), and pertains to the field of molecular markers. To address the current difficulty in identifying sex-reversed pseudomale individuals in the Northeast Forest Frog, this invention extracts the genome from the muscle tissue of the left hind limb of the Northeast Forest Frog. Using the extracted genomic DNA as a template, PCR amplification is performed using primer pairs containing the sequences shown in SEQ ID NO:1 and SEQ ID NO:2. The PCR amplification products are detected by electrophoresis on a 3% agarose gel. Phenotypic male individuals exhibiting a 513bp band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are genetically male Northeast Forest Frogs, i.e., true males. Phenotypic male individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are pseudomale individuals exhibiting sex reversal from genetically female to phenotypically male. Phenotypic female individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are female individuals.

This invention relates to the molecular marker M-866 and its application in the identification of sex-reversed pseudomale individuals in the Northeast Forest Frog (Rana davidii), and pertains to the field of molecular markers. To address the current difficulty in identifying sex-reversed pseudomale individuals in the Northeast Forest Frog, this invention extracts the genome from the muscle tissue of the left hind limb of the Northeast Forest Frog. Using the extracted genomic DNA as a template, PCR amplification is performed using primer pairs containing the sequences shown in SEQ ID NO:1 and SEQ ID NO:2. The PCR amplification products are detected by electrophoresis on a 3% agarose gel. Phenotypic male individuals exhibiting the 866bp band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are genetically male Northeast Forest Frogs, i.e., true males. Phenotypic male individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are pseudomale individuals exhibiting sex reversal from genetically female to phenotypically male. Phenotypic female individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are female individuals.

The invention provides an intelligent wood frog breeding environment regulation and control system, and relates to the technical field of agricultural product related Internet of Things control systems, the technical scheme is characterized in that the system comprises a multi-dimensional sensing module, an AI decision processing module, an execution module and a remote interaction module, the multi-dimensional sensing module is in communication connection with the AI decision processing module, and the remote interaction module is in communication connection with the AI decision processing module. The AI decision processing module is respectively connected with the execution module and the remote interaction module; the multi-dimensional sensing module is used for collecting environmental parameter data, wood frog state data, external interference data and micro-ecological data; the AI decision processing module generates specific regulation and control instructions based on historical data and real-time data, and the regulation and control instructions at least comprise an emergency relieving instruction, a temperature and humidity regulation and control instruction, a light intensity regulation and control instruction and a feeding instruction. The wood frog culture environment intelligent regulation and control system provided by the invention has the advantages that multi-dimensional perception is realized, and intelligent pre-judgment and real-time accurate refined regulation and control of the wood frog culture environment are realized.

This invention relates to the field of artificial breeding technology, specifically to a feed additive for the fully artificially bred Northeast Forest Frog and its application. The feed additive includes dandelion and Bacillus licheniformis, wherein the mass ratio of dandelion to Bacillus licheniformis is (0.02~0.1):1. The feed additive provided by this invention can effectively control digestive diseases in forest frog tadpoles, improve intestinal flora, promote weight gain and digestion in forest frog tadpoles, significantly improve the metamorphosis effect of forest frog tadpoles, and increase the survival rate of metamorphosed forest frog larvae.

The invention relates to the technical field of artificial breeding, in particular to a feed additive for fully artificially bred rana chensinensis and application of the feed additive. The feed additive comprises dandelion and bacillus licheniformis, and the mass ratio of the dandelion to the bacillus licheniformis is (0.02-0.1): 1. The feed additive provided by the invention can effectively control the occurrence of digestive diseases of wood frog tadpoles, improve intestinal flora, promote weight gain and digestion of wood frog tadpoles, and significantly improve the metamorphosis effect of wood frog tadpoles and the survival rate of metamorphosed wood frog larvae.

A kind of forest frog oil freeze-dried powder discharging equipment, including transfer device (100) and variable-pitch device (200) installed on transfer device (100) are constituted, the transfer device (100) includes rack (110), swing lever (160) rotationally connected rack (110), rotary lever (120) rotationally connected swing lever (160), square bar (130) slidingly connected rotary lever (120), hanger (150) rotationally connected square bar (130) by gear, the variable-pitch device (200) includes variable-pitch seat (210), variable-pitch shaft (220) rotationally connected variable-pitch seat (210), moving frame (230) slidingly connected variable-pitch shaft (220);So that the product on main conveying belt 320 realizes continuous distribution under the condition of not stopping, greatly improve the efficiency of forest frog oil freeze-dried powder material distribution.

The invention relates to the technical field of aquaculture, and particularly discloses a nutritional feed formula for efficiently promoting the development of wood frog tadpoles. The composition comprises a plant protein source, an animal protein source, a carbohydrate source, a fat source, a vitamin premix, a mineral premix, a probiotic additive, an algae extract, an enzyme preparation, a natural antioxidant and the like. The preparation method comprises the following steps: pretreating the basic nutritional raw materials, carrying out primary mixing, then carrying out step-by-step intensified mixing, adding the heat-sensitive functional components under the protection of inert gas, and then carrying out fermentation, microwave and ultrasonic composite sterilization, granulation and post-treatment. According to the composition disclosed by the invention, through scientific compatibility of various components, the comprehensive nutritional requirements of the wood frog tadpoles in each development stage can be met; besides, according to the preparation method disclosed by the invention, a step-by-step mixing technology and an activity protection technology are combined, so that the biological activity of functional components in the feed is effectively maintained, and the stability and the feeding value of a feed product are improved.

The invention relates to the technical field of wood frog hatching environment management, in particular to a wood froglet hatching environment management method and system, and the method comprises the steps: collecting various hatching data of a wood froglet hatching environment in each time sequence interval, and dividing each hatching sequence in the time sequence interval into positive hatching secondary sequences and negative hatching secondary sequences; constructing a positive partial weight factor of the incubation sequence corresponding to each positive category and a negative partial weight factor of the incubation sequence corresponding to each negative category in the feature interval, and obtaining a bidirectional influence incubation coefficient of the feature interval according to the positive partial weight factor and the negative partial weight factor; constructing a data smoothing factor of each feature interval in each incubation cluster in the time sequence interval; calculating a fusion effective factor and an incubation effective correlation coefficient of each incubation cluster in the time sequence interval; and monitoring and managing the wood froglet hatching environment in combination with a support vector machine model. According to the invention, accurate monitoring of the wood froglet hatching environment is realized.

The invention relates to the technical field of frog egg collection, in particular to an oviposition induction and egg collection integrated device for northeast wood frog breeding, which comprises a base, a cross beam is arranged above the base, the base and the cross beam are fixedly mounted through vertical plates, a switching plate is fixedly mounted between the two vertical plates, and the switching plate is fixedly mounted on the base. An egg groove is fixedly formed in the side wall of the switching plate, supporting legs are installed at the lower end of the egg groove, a collecting groove is formed in the upper portion of the base, a switching mechanism used for switching the position of the collecting groove is arranged on the base, an egg taking mechanism used for taking and placing frog eggs is arranged on the cross beam, and a material spraying box is arranged above the egg groove. And an adjusting mechanism for adjusting the material spraying box is arranged on the cross beam. Compared with the prior art, wood frog spawns in the same time period can be collected, the survival rate of the wood frog spawns is increased, wood frogs are uniformly induced and fed, and the spawning rate of the wood frogs is increased.

This invention relates to molecular marker 897 and its application in the identification of sex-reversed pseudomale individuals in the Northeast Forest Frog (Rana davidii), and pertains to the field of molecular markers. To address the current difficulty in identifying sex-reversed pseudomale individuals in the Northeast Forest Frog, this invention extracts the genome from the muscle tissue of the left hind limb of the Northeast Forest Frog. Using the extracted genomic DNA as a template, PCR amplification is performed using primer pairs containing the sequences shown in SEQ ID NO:1 and SEQ ID NO:2. The PCR amplification products are detected by electrophoresis on a 3% agarose gel. Phenotypic male individuals exhibiting the 897 bp band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are genetically male Northeast Forest Frogs, i.e., true males. Phenotypic male individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are pseudomale individuals exhibiting sex reversal from genetically female to phenotypically male. Phenotypic female individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are female individuals.

The invention discloses an efficient and safe wood frog pest and disease damage and aquatic tadpole natural enemy control agent, and relates to the technical field of wood frog culture. In order to solve the problem that insects threatening wood frog tadpoles cannot be effectively killed, so that the functionality of the wood frog prevention and treatment medicine is relatively low. The pesticide specifically comprises 80 parts of a double-chain quaternary ammonium salt complex iodine solution with the concentration of 5%, 10 parts of a deltamethrin solution with the concentration of 20% and 10 parts of a fenvalerate solution with the concentration of 8%, a solvent of the deltamethrin solution is an ethyl acetate solution, and a solvent of the fenvalerate solution is a methanol solution. The wood frog and tadpole trapping prevention and control agent can effectively kill and control beetles, larvae, kyllinga chinensis, dragonfly larvae, backstroke bugs and other insects capable of preying on wood frog and tadpoles in a culture pond, so that the prevention and control agent can effectively prevent and control wood frog infectious diseases and effectively kill natural enemy pests threatening wood frog and tadpole survival; the survival rate of wood frogs is improved.

The present application belongs to the technical field of biological gene engineering, and relates to a Rana chensinensis lysozyme gene, a mature polypeptide region gene, a recombinant protein and a recombinant lysozyme genetically engineered bacterium, and application of the recombinant lysozyme genetically engineered bacterium. The present application provides a Rana chensinensis lysozyme gene with a sequence shown in SEQ ID NO. 1 and a Rana chensinensis lysozyme mature polypeptide region gene with a sequence shown in SEQ ID NO. 4. After the mature polypeptide region gene is connected to a vector and transformed into Escherichia coli BL21 (DE3), a recombinant lysozyme genetically engineered bacterium for expressing a recombinant protein is obtained, and the bacteriostatic ability of the recombinant lysozyme genetically engineered bacterium on Micrococcus luteus, Staphylococcus aureus, Escherichia coli and Aeromonas hydrophila from amphibians is detected. The Rana chensinensis lysozyme provided by the present application has bacteriostatic ability on Micrococcus luteus, Aeromonas hydrophila, Escherichia coli and Staphylococcus aureus, and can be used for developing antibacterial drugs and feed additives.

This invention relates to the molecular marker M-994 and its application in the identification of sex-reversed pseudomale individuals in the Northeast Forest Frog (Rana davidii), and pertains to the field of molecular markers. To address the current difficulty in identifying sex-reversed pseudomale individuals in the Northeast Forest Frog, this invention extracts the genome from the muscle tissue of the left hind limb of the Northeast Forest Frog. Using the extracted genomic DNA as a template, PCR amplification is performed using primer pairs containing the sequences shown in SEQ ID NO:1 and SEQ ID NO:2. The PCR amplification products are detected by electrophoresis on a 3% agarose gel. Phenotypic male individuals exhibiting a 994 bp band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are genetically male Northeast Forest Frogs, i.e., true males. Phenotypic male individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are pseudomale individuals exhibiting sex reversal from genetically female to phenotypically male. Phenotypic female individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are female individuals.

The invention belongs to the technical field of deep processing of agricultural and sideline products, and particularly relates to an extraction method of wood frog line oil. The extraction method comprises the following steps: putting female wood frogs into water for gut cleaning treatment, and then putting the female wood frogs into warm water for blood discharge treatment; taking out the oviduct of the wood frog after the blood discharge treatment, removing impurities on the surface of the oviduct, and then putting the oviduct into water for cleaning; oil is smeared on the surface of the cotton thread, the fallopian tube with water drained is stretched to be straight, the linear fallopian tube is hung on the cotton thread, and the hung and shaped fallopian tube is air-dried; and taking down the dried wood frog line oil from the cotton thread, cooling the wood frog line oil to room temperature, sealing and storing the wood frog line oil, and finally sterilizing and disinfecting the wood frog line oil. According to the invention, blood discharge treatment is carried out before fallopian tube stripping, so that the color and quality of the product are fundamentally improved; by adding the step of sterilization after drying, the hygienic safety of the product is ensured, the shelf life is prolonged, and the standardized production of the wood frog line oil is realized.

The present application belongs to the field of biological health care products and food processing technology, and relates to a testosterone inducing liquid of Rana spinosa, testosterone-enriched Rana spinosa polypeptide, and a preparation method and application thereof. The present application carries out multi-disciplinary and multi-factor intervention experiments on Rana spinosa in the breeding period, explores the estrus information substance of Rana spinosa, finds an effective method for stimulating the estrus of Rana spinosa in the breeding period by using the information substance of female frogs, and facilitates the production and application of raw materials, thereby creating a new path for the development of functional products of Rana spinosa. Through artificial intervention and induction, the testosterone content of Rana spinosa in the breeding period is synchronously increased, and the testosterone level is more than twice that of the control group. The present application further provides a production process for extracting polypeptide from Rana spinosa, including the steps of raw material storage, enzymolysis, fermentation, centrifugation, drying, and the like, and finally obtaining a testosterone-enriched polypeptide product. The product can be applied to the fields of food, health care products, and medicine, and has the potential to improve the economic value of Rana spinosa.