51 results about "Rana sylvatica" patented technology

A molecular identification method for the wood frog in the northeast of China and the ovum oil thereof aims at solving the identification of the wood frog in the northeast of China and the product ovum oil thereof, which is mainly based on the property characteristics, microscopic characteristics, physical and chemical properties, and the like at present; as the bases are lack of clear shape identification characteristics and special chemical compositions, the identification for the ovum oil in practice is still very difficult and the invention is hereby designed. The identification method takes PCR technology as the basis and screens specific primers for amplification so as to achieve the molecular identification of the wood frog in the northeast of China and the ovum oil thereof, comprising the following steps: (1) extraction of genomic DNA; (2) genomic DNA detection and concentration determination; (3) PCR amplification; and (4) PCR amplification product identification. The invention is characterized in that the invention starts from the basic theory of molecular biology, uses modern molecule marker technology for conducting molecular identification of the wood frog in the northeast of China and the ovum oil thereof, has the advantages of scientific and credible theoretical basis, advanced and reasonable technical means and achieving steps, reliable detection result, strong specificity of screened primers as well as high stability of agarose gel electrophoresis characteristic bands, and can obtain reliable and useful effects in practical application.

The invention provides a wood frog stereoscopic cultivation technique. The wood frog stereoscopic cultivation technique relates to a wood frog cultivation ecological system, a wood frog anti-escape method, a wood frog cultivation harm prevention method and a feeding method for increasing the wood frog survival rate and the oil yield. The facilities used in the wood frog cultivation ecological system comprise a frog house and a grape trellis. A wall used in the wood frog anti-escape method is built by stones or bricks, the wall is of a T shape, and the top of the wall is covered by an inverted U-shaped alloy aluminum plate. The wood frog cultivation harm prevention method comprises the method for preventing harm caused by snakes, mice and cats and the method for preventing harm caused by birds. The feeding method for increasing the wood frog survival rate and the oil yield is achieved through the following steps that (1) after being hatched, firstly, tadpoles eat egg-covered egg membranes for 7-10 days, and then the tadpoles eat bait after eating up the egg membranes; (2) after the tails of froglets are completely absorbed, small yellow mealworms which are 0.6-0.8mm in length begin to be utilized to feed the froglets; (3) after 10-15 days, yellow mealworms which are 1-1.2mm in length are utilized to feed the froglets; (4) after three months, the yellow mealworms which are 1.5-2mm in length are utilized to feed the froglets.

The invention relates to a multi-in-one cultivation simple building for China wood frog. Said simple building comprises building body, roof and pool inside room; the lower building body is complete closed, the upper building body is provided with pillar on lower building body, and is circled with sun-shading net around pillar, the outside of sun-shading net is equipped with movable curtain; the roof is straw roof and has two sides along ridge; the pool inside the room comprises cultivation pool and laying-up basin, each cultivation pool is provide with a overflow bench, the bench surface is equipped with a cushion and the cushion is fixed on slope bench with a plurality of bracing column of same height, and a bamboo curtain is placed on the bracing column for young wood frog rest. The said multi-in-one employs cultivation technique of supporting facilities of temperature control, humidity control, disinfection and bait feed in one cultivation pool, and finishes whole courses of oviposition, hatching, polliwog coming out of film, and young frog growth into product frog and hibernation. The survival rate is above 80%, adult frog can be sold in the same year, the investment is saved and cultivation scale can be enlarged.

The invention provides adult granulated feed of northeast wood frog, which comprises the following components in parts by weight of 35-60 parts of fish meal, 0.4-0.6 part of starch, 0.2-0.4 part of yolk powder, 22.5-37.4 parts of bean pulp, 4.2-4.8 parts of fish oil and 0.1-0.4 part of vitamin microelement additive. In the invention, a proper amount of traditional Chinese medicine and western medicine can be added in the feed formulation according to the antiviral, antibiosis and antiphlogosis requirements to form a functional formulation. The adult granulated feed of northeast wood frog is prepared based on the nutrition constituent formed by the natural food of northeast wood frog, can fully satisfy the requirement on the northeast wood frog for the main nutrition substances, can substitute more than 95% chum food, reduces the cost by 50% above compared with chum feed, is easily to store, reduces the occupation of manpower and space and supplements the breeding industry of northeast wood frog.

A stereoscopic cultivation and breeding method for complementary symbiosis of ganoderma lucidum and wood frogs is characterized by comprising the steps that a good, pure-natural and pollution-free area section is selected, it is ensured that the area section is free of residues, soil is not too alkaline and the area section is a loose sandy soil land section, deep land scarification is performed for 40 centimeters in the first year, leveling is performed, and a ganoderma lucidum and wood frog greenhouse is constructed after six months of sun exposure.Too large area is not allowed, the area of the greenhouse is 200 square meters, the length of the greenhouse is 25 meters, the width of the greenhouse is 8 meters, the height of the greenhouse is 1.9 meters, the area is suitable for a growing environment for the complementary symbiosis of the ganoderma lucidum and the wood frogs, the greenhouse is suitable for conidial powder recovery, the area of the greenhouse is suitable for the wood frogs due to the fact that wood frogs breathe through the skins, the ganoderma lucidum breathes freely, the wood frogs uptake oxygen, the complementary symbiosis is achieved, and the greenhouse is disinfected and sterilized with quick lime water after the greenhouse construction is completed; ganoderma lucidum mycelium in the step 2 is ganoderma lucidum.

The invention provides a wood frog breeding method, which comprises the steps of spawning management, egg hatching management, comprehensive management and breeding in the processes that wood frogs become tadpoles, and the tadpoles are changed to be froglets, and the like. The method is implemented in spawning and hatching periods according to the most suitable conditions of the wood fogs, so that the phenomena that due to poor management in an earlier stage, the tadpole conversion rate is reduced, and the wood frog forming rate is further reduced are avoided.

The invention provides a Chinese wood frog bionic bait device. The device consists of a machine frame transverse-motion mechanism, a flexible bait disk, a low-speed DC motor, a transmission shaft bracket, cams and transmission shafts, wherein the transmission shaft is provided with a certain number of eccentric cams, and a certain phase is mutually formed between the adjacent cams; the cams arranged on the corresponding positions of the two adjacent transmission shafts have an initial phase difference; the transmission shaft and a cam system are driven to rotate by the low-speed DC motor to force the flexible bait disk to generate vertical low-frequency small vibration according to a rule, and the new bait which is manually synthesized creeps along a spiral line track under an action of the low-frequency small vibration, so that the simulation of continuous actions of the worm bait is achieved, and the problem of single nutrient content in the bait existing in the conventional Chinese wood frog breeding is solved; and simultaneously, the device also has the advantages of effectively improving breeding environment, lowering breeding cost, improving quality of frog oil of the wood frog, and having prominent effect of realizing the Chinese wood frog industrialized large-scaled breeding technology.

The feeding method for improving the survival rate and the oil extraction rate of wood frogs comprises the following steps that after tadpoles are hatched, the tadpoles are fed by egg membranes containing eggs for 7 to 9 days, and after the egg membranes are eaten up, the tadpoles are fed by bait as which cooked egg yolk or soybean milk serves, and the tadpoles are fed by the bait one time every day; after the tail of each froglet is completely absorbed, the froglets are fed by small flour weevils with the length ranging from 0.6cm to 0.8cm, a black light lamp is used for luring natural insects to be auxiliary bait, and the froglets are fed one time in the morning and one time in the evening; after 10-15 days, the froglets are fed by flour weevils with the length ranging from 1cm to 1.2cm and are fed twice every day; after 3 months, the frogs are fed by flour weevils with the length ranging from 1.5cm to 2cm and are fed twice to three times every day. By means of the method, the survival rate of the wood frogs is improved to 90%, and the oil extraction rate is increased by 20% on the existing basis.

The invention relates to the technical field of Rana chensinensis breeding and especially relates to a breeding shed for Rana chensinensis and a breeding method thereof. A breeding shed for Rana chensinensis comprises a fence and a ceiling which are characterized in that the ceiling is installed at the upper end of the fence; the fence is a rectangle framework structure formed by multiple fixed piles and nylon window screen; the internal fence is provided with a living area for Rana chensinensis in the center, a trench is arranged at the peripheral of the Rana chensinensis living area, and a walkway is installed at the peripheral of the trench; the ceiling presents an umbrella-shaped structure, a part, from the center to the two-thirds of the periphery, of the ceiling is a weak light layer, and a part, from the periphery to the one-third of the center, of the ceiling is a dark layer. Compared with the prior art, the breeding shed for <{EN0}>Rana chensinensis and the breeding method thereof has simple and secure structure, is easy to construct on site, and has good cooling and ventilation effect; the pavilion-type fence shed satisfies the demand of free lighting, feeding on food platform, motion and comfortable inhabitation; temperature and humidity are adjustable; the cooling and moisturizing effect is obvious in hot summer days so that <{EN0}>Rana chensinensis can safely live through summer.

This invention relates to a method for recovering the forest frog's oviduct in a non-harmful way, belonging to the technological field of acquiring proteins from animal eggs. The process comprises of artificial spawning stimulation, centrifugal separation, acquiring egg capsules and egg cells, and putting back for hibernation. Concretely, it comprises: fishing fifty-fifty male and female wood frogs for centralized breeding before the wood frog's hibernation, leaving the wood frogs to mate and lay eggs half a month later, centrifuging the eggs, collecting the egg capsules from the white upper layer and the egg cells from the black sublayer respectively, and putting the male and female wood frogs back to the watershed for their hibernation. This invention is characterized in that, by using artificial spawning stimulation and centrifugal separation technologies to recover egg caplsules(forest frog's oviduct) in winter non-breeding season, it thoroughly changes the traditional process of recovering egg caplsules by killing the wood frogs, covers the shortage of current technology-oviduct recovering method from living wood frogs, makes it more qualified for commercial batch production, makes full and efficien use of wood frog resources, improves economic benefits of wood frog production and protects the ecologic environment as well.

The invention discloses a method for cultivating a wood frog by breeding an oxya through an ecological fence, mainly comprising the following steps: (1) selecting a wood frog cultivation site; (2) building a fence; (3) collecting and storing oxya oocyst; (4) building a grassland for breeding the oxya; (5) building an oxya enlarged breeding room; and (6) taking the oxya as main insectivora to cultivate the wood frog. The method of the invention is a semi-naturalized cultivation model, does not change the ecological predation habit of the wood frog, avoids occurrence of epidemic pests and diseases while artificially breeding the wood frog, and has the advantages of simple basic facilities, simple and convenient operation, small investment, great income, no territorial restriction, easy popularization and implementation and the like.

The invention provides a molecular marker for identifying genetic sex of rana dybowskii. The method is mainly characterized by comprising the following steps: extracting a genome of a toe tissue of rana dybowskii, and carrying out PCR (Polymerase Chain Reaction) amplification reaction by taking an extracted genome DNA (Deoxyribonucleic Acid) as a template and a primer pair containing sequences asshown in SEQ ID NO:1 and SEQ ID NO:2; and carrying out electrophoresis detection on a PCR amplification product through 14% polyacrylamide gel, wherein the individuals with bands corresponding to a nucleotide sequence (222bp) as shown in SEQ ID NO:3 are genetic male rana dybowskii, and the individuals without the bands corresponding to the nucleotide sequence as shown in the SEQ ID NO:3 are genetic female rana dybowskii. The method has the advantages that the genetic male specific DNA molecular marker of the rana dybowskii is obtained, and the genetic sex of the rana dybowskii can be quickly,simply, conveniently and accurately identified. Pseudo-male individuals, namely the individuals which are physiologically male but inherited as females, can be obtained. The pseudo-male individuals are used for mating with female individuals, and the proportion of physiologically female progenies is as high as 90% or above, so that economic benefits of rana dybowskii breeding can be remarkably improved.

The invention, which relates to the field of molecular markers, discloses a molecular marker for identifying genetic sex of rana dybowskii and application thereof, thereby solving a problem that the genetic sex of the rana dybowskii cannot be identified at present. The genome of the toe tissue of the rana dybowskii is extracted and PCR amplification reaction is performed by using the extracted genome DNA as a template and a primer pair containing sequences shown in SEQ ID NO: 1 and SEQ ID NO: 2; electrophoresis detection is performed on a PCR amplification product by using 14% of polyacrylamide gel; and the amplification result shows that the individual with a band corresponding to a nucleotide sequence (261 bp) shown in SEQ ID NO: 3 is a genetic male northeast rana dybowskii and the individual without a band corresponding to the nucleotide sequence shown in SEQ ID NO: 3 in the amplification result is a genetic female northeast rana dybowskii. Therefore, pseudo-male individuals, namelyindividuals which are physiologically male but inherited as female, can be identified by the method. Pseudo-male individuals and female individuals are used for mating, and the physiological female proportion of the generated progeny reaches up to over 90%. The sex of the rana dybowskii breeding group can be controlled, and the economic benefit of rana dybowskii breeding is improved.

The invention provides a method for increasing the number of female wood frogs in field bred wood frog population, which comprises the steps of construction of an embryo development tank, a tadpole development tank, a water sun-drying tank and a wastewater reclamation tank, determination and regulation of water environment pH value, salinity, alkalinity, H2CO3 concentration, Ca<2+> / Mg<2+> ratio, Ca<2+> + Mg<2+> total concentration and Na<+> / K<+> ratio, regulation of favorable water temperature, and wastewater reclamation and recycling. By using the method provided by the invention, the proportion of female wood frogs in the wood frog population bred in a field ecological sealed trench is increased to 62.75%, with an increase of 19.56% compared to the proportion of the female wood frogs in natural wood frog population, the annual mean recapture number of wood frogs is increased to 69.98%, and the mean proportion of female wood frogs in annual bred recaptured wood frog population is 65.68%. The method is an important technology in wood frog industrial high-efficiency breeding.

The invention provides a coating material, a hermetia illucens larva living thing coating bait, and a preparation method and application, and belongs to the techncial field of application of industrial aquaculture baits. The coating material comprises the following preparation raw materials of defatted soy flour, antheraea pernyi powder, soybean oil, corn starch, multivitamin and minerals tablets,calcium, phosphorus, magnesium, zinc and ferrum rapidly enriching tables for livestock and xanthan gum. The coating material is reasonable in matching of nutrient components, and the hermetia illucens larva living thing coating bait prepared from the coating material can meet requirements of Chinese forest frogs for growth, development, oil generation and propagation. The hermetia illucens larvaliving thing coating bait solves the problems that at current, the forest frog living insect bait is single in kinds, and unbalanced in nutrients, the cost of the baits is greatly reduced, and the content of nutrient components required by the forest frogs can be manually controlled and balanced, so that the oil quality of forest frogs which are artificial bred and the oil quality of wild forest frogs are not obvious in difference, the oil generating period is shortened by 30%, and the hermetia illucens larva living thing coating bait has important significance in realization of industrial large-scale cultivation techniques of the Chinese forest frogs.

A monosexual wood frog cultivation process wherein from tailbud stage to gill cover completion stage, female hormone with concentration of 0.5 mg per liter water is applied, the female mutagenesis ratio is 80-100%, and no influence to polliwog metamorphosis and wood frog growth is found, the advantages of the invention are wide range of gender transformation and large benefit dosage range.

The invention relates to a method for collecting frog oil from live forest frogs. The method comprises the following steps: cleaning live female forest frogs, hanging upside-down on a conveyor belt, cutting frog heads for bleeding, hanging frog bodies in an orthotopic manner, cutting open abdomens, recovering egg oil, removing internal organs, separating skin from flesh, separating the egg oil, drying the frog oil at lower temperature, vacuum-sealing, and finally obtaining a bagged dry product of forest frog oil. The application of the method for collecting the frog oil from the live forest frogs is applicable to industrial production, and the obtained forest frog oil has the advantages of great activity, high purity, good product appearance, pure taste, no peculiar smell and convenient use. The product is easy to preserve, and the preservation time is relatively long, which can be up to two years at room temperature. As the frog oil is collected from the live forest frogs, the somatic cells of the forest frogs are not dead yet, and the forest frog bodies and the forest frog eggs have high activity, thereby remaining a very large development space for further development of the forest frog eggs, the forest frog bodies and the forest frog skin.

The invention discloses a processing technology of instant Chinese forest frog. The raw materials of the instant Chinese forest frog comprise dry rana japonica oil, lotus seeds, red dates, Chinese wolfberry, honey and honeysuckle powder. The preparation method comprises the following steps that the dry rana japonica oil is subjected to impurity removal and crushed into powder, water is added, ozone is introduced at the same time, dipping and soaking are carried out at 6-10 DEG C for 10-15 h, and dehydration and fishy smell removal are carried out; the lotus seeds, the red dates and the Chinese wolfberry are added into boiled water, cooking thoroughly, taking out and mashing are achieved, dry rana japonica oil powder is added, and the honey and honeysuckle flower powder are added while stirring; and the formed mixture is put into a jacketed kettle, 200-300 parts of water is added, heating is achieved to fully mix and dissolve, filtering is achieved to remove impurities when the water is concentrated to 30-40 times of the instant Chinese forest frog raw material, cooling and bottling are achieved, and sealing and sterilizing are achieved at high temperature to obtain the product. According to the processing technology of the instant Chinese forest frog, the problem of single eating method of wood frogs is solved, the Chinese forest frog food which is delicious in taste and rich in nutrition is provided, and the Chinese forest frog food is similar to jelly in mouth feel, rich in nutrition, fresh, cool and slightly sweet in the mouth feel and has a certain health-care effect.

The invention relates to pine mushroom wood frog oil, formed by wood frog oil, pine mushroom and sauce. The preparation comprises that 100 deals of pine mushroom wood frog oil food contain 10-30 deals of pine mushroom wood frog oil, 1-15 deals of pine mushroom, and the left sauce. The invention uses the pine mushroom wood frog oil in ball shape and 10-20mm diameter; therefore, the frog oil can berecognized. And the invention adds different sauce to meet different tastes. And the invention can use non-sugar wood frog oil for diabetic patient. The invention uses vacuum package and instant high-temperature disinfection to prolong the storage time.

The invention discloses a formula of artificial food prepared from silkworm pupa meal and moth meal and used for wood frogs and tadpoles and a preparation method of the formula. The formula comprisescomponents as follows: 8-12 parts of silkworm pupa meal, 6-10 parts of female silkworm moth meal, 10-35 parts of corn flour, 15-25 parts of bran, 15-30 parts of soybean meal, 8-20 parts of bone meal,3-15 parts of fish meal, 3-7 parts of radix puerariae powder, 1-3 parts of compound probiotics and 2-4 parts of yeast powder. With the adoption of the technical scheme, the problems that the wood frogs and the tadpoles eat less, grow slowly and have high fatality rate and low survival rate and few females exist can be solved, the existence rate can be increased by 20%, and the female rate can be increased by 10%. The method has the advantages that the formula is reasonable, the product is mechanically processed into granules, a process is simple, the artificial food is convenient to conserve and transport, feeding is convenient, the cost is low, and the artificial food is easy to accept by farmers.

The invention provides a production method of modified oviductus ranae, which comprises the steps of oviposition in purified water, fertilized egg treatment, freeze drying, oil-egg separation and the like, and the obtained oviductus ranae is the modified oviductus ranae. According to the method, pure and impurity-free oviductus ranae without sex hormones is extracted under the condition of not needing excessive external intervention according to the embryonic development characteristics of Rana amurensis, and the obtained oviductus ranae does not have the characteristics of form, component content, medicine property and the like of traditional oviductus ranae, but is similar to the traditional oviductus ranae in water absorption expansion rate and smell. The protein content is inversely proportional to the time before the embryonic development is completed, and the contents of polypeptides, oligopeptides and amino acids are directly proportional to the time before the embryonic development is completed. The method does not damage the health and normal oviposition and survival of the wood frogs, prolongs the utilization cycle of the wood frogs, upgrades the traditional oil extraction by killing the frogs into oil extraction by raising the frogs, and has important significance for protecting the precious germplasm resource of the wood frogs in China, orderly developing and reasonably utilizing the wood frogs and benefiting the society.

The invention discloses a DNA extraction and identification method of forest frog oil. A base sequence of a Rana dybowskii COI3 gene is as shown in SEQ ID NO.1, and the COI3 gene is applied to identify Rana dybowskii oil. The method comprises the following steps: performing PCR amplification on a target gene by using specific primers and probes; taking 5 [mu]L of a PCR product after a reaction isfinished, and performing agarose gel electrophoresis detection; and recovering the amplification product and obtaining sequencing results: the amplification products obtained by performing PCR on 16 Rana dybowskii samples by virtue of mitochondria COI primers are subjected to forward and reverse sequencing to respectively obtain two about 311 bp fragments, a sequence segment with a size of about 230 bp is obtained after the comparison and correction of a DNAstar software, and the consistency of a sequence of the sequence segment of each Rana dybowskii sample, and a Rana dybowskii COI sequenceis 99% through the BLAST comparison.

The invention discloses a young frog management technology for wood frogs. The management technology is characterized in that bleaching powder of 1 ppm is used for disinfection of whole fences beforeyoung frogs enter a frog house; the young frogs are placed into the frog house according to the density of 300-400 frogs in each square meter of the frog house; water is sprayed once every day, and the amount of the water is not too much so that accumulated water on the ground is prevented; feeding is carried out from 4 a.m. to 5 a.m. in the morning or from 5 p.m. to 6 p.m. in the afternoon everyday; each young frog is fed by using 1-2 tenebrio molitors of 2-3 instars every day; after 40 days, feeding is carried out twice every day by using 2-3 tenebrio molitors of 4-5 instars; and corn stalks or tree branches and leaves and the like are placed on the periphery of the frog house, so that the landing of the young frogs are facilitated. The management technology solves the problem that whenwood frogs are bred in coastal regions, the survival rate of the young frogs is low because of the climate environment, so that the management technology can be widely adaptable to coastal regions.

Provided is an adult frog management technology for wood frog breeding. The adult frog management technology for wood frog breeding is characterized by including the steps that adult frogs are released according to the density of 40-50 per square meter of a frog pen, larvae of tenebrio molitor are released as main feed, feeding is conducted once every day at 4-7 AM or 5-7 PM, and each wood frog isfed with 3-4 larvae each time. By means of the adult frog management technology for wood frog breeding, the problem is solved that growth is slow in frigid regions, so that the social requirements are not met; the adult frog management technology for wood frog breeding is widely suitable for being used in coastal regions.

A seedling management technique of wood frog is characterized by comprising steps of sterilizing the whole fence by 1 ppm chlorinated lime before froglets enter a frog house; stocking froglets according to the density of stocking 300-400 froglets in every square meter of the frog house; spraying water once every day and do not spray too much so as to prevent waterlog at the ground; feeding at every morning 4-5 a.m. or afternoon 5-6 p.m.; feeding 1-2 yellow mealworms at 2-3 years old for every froglet every day; feeding for two times every day after 40 days; feeding 2-3 yellow mealworm at 4-5 years old; placing corn stem, branch leaves and other at surrounding of the frog house for helping froglets to go ashore; the technique solves the problem of low survival rate due to the climate environment when the frogs are cultivated in the coastal areas, and is widely applicable to the coastal areas.

The invention provides a frigid zone wood frog cultivation technique in a coastal region. The frigid zone wood frog cultivation technique in the coastal region is characterized by comprising the following steps that froglets are bred, wherein the froglets are placed in a frog fold with a density of 300-400 froglets per square meter, every froglet is fed with 1-2 yellow mealworms which are 2-3 years old every day, and every froglet is fed with 2-3 yellow mealworms which are 4-5 years old twice every day after 40 days; corn stalks or branches and leaves are placed surrounding the frog fold so that the froglets can go ashore conveniently; mature frogs are bred, wherein the mature frogs are placed in the frog fold with a density of 40-50 mature frogs per square meter, feed cast to the mature frogs is mainly yellow mealworm larvae, and every wood frog is fed with 3-4 yellow mealworm larvae once every day between 4 and 7 in the morning or between 5 and 7 in the afternoon. The frigid zone wood frog cultivation technique solves the problems that wood frogs grow slowly in a frigid zone and social requirements for the wood frogs cannot be met, and the frigid zone wood frog cultivation technique is suitable for being widely used in coastal regions.

The invention provides a coating material, coating bait for live black soldier fly larvae, a preparation method and application thereof, and belongs to the technical field of industrialized breeding bait application. The coating material of the present invention includes the following raw materials for preparation: defatted soybean powder, tussah moth powder, soybean oil, corn starch, multi-dimensional element tablets, calcium, phosphorus, magnesium, zinc and iron for veterinary rapid supplementation and xanthan gum. The nutritional components of the coating material of the invention are reasonably configured, and the coating bait for living black soldier fly larvae prepared by the coating material of the invention can meet the needs of growth, development, oil production and reproduction of Rana chinensis. The black soldier fly larvae live coated bait of the present invention solves the problem of single species and unbalanced nutrition of the live insect bait of the forest frog, greatly reduces the cost of the bait, artificially controls and balances the required nutrient content of the forest frog, and makes artificial There is no significant difference in the oil quality of the cultivated wood frog and the wild wood frog oil, and the oil production cycle is shortened by 30%, which is of great significance to the realization of the industrial scale breeding technology of the Chinese wood frog.