In-situ gel formation of pectins
A pectin and gel technology, which is applied in the fields of peptide/protein components, non-active components of polymer compounds, and medical preparations of non-active components, etc., can solve problems such as being unsuitable for human and animal medical use
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Embodiment 1
[0066] In situ gelation of aloe pectin
[0067] Extraction of Aloe Vera Pectin
[0068] Aloe pectin is extracted from cell wall fibers prepared from the serum or rind of aloe vera leaves. Basic methods for extracting pectin have been reported. See Voragen et al., In Food polysaccharides and their applications, pp. 287-339, Marcel Dekker, Inc., New York, 1995, and see also US Patent No. 5,929,051, which is expressly incorporated herein by reference in its entirety. Using chelating agents such as EDTA or in the presence of hot water, hot dilute acid (HCl, pH 1.5-3) and cold dilute alkali (NaOH and NaOH 2 CO 3 ; pH10) and other conditions to extract aloe pectin.
[0069] After extraction, residual fibers are removed by coarse and fine filtration. Precipitate the pectin with ethanol. The pectin precipitate was further washed with ethanol solution and then dried.
[0070] The characteristics of the aloe pectin obtained by this method from the pulp or the cell wall fibers of ...
Embodiment 2
[0080] In situ gelation following topical administration on wound surfaces
[0081] The aloe pectin preparation (0.5%, w / v) in saline was directly applied to the entire cut wound on the skin of mice or rats. A formulation containing 0.5% (w / v) CMC in saline and a commercial hydrogel wound dressing were used as controls. Wounds were created using the in vivo percussion method following animal use protocols. After 4 hours, the rats were sacrificed and the wounds were surgically removed. Wounds were fixed in formalin, then sectioned and stained with H&E. A gel layer was obviously formed on the wound surface with the aloe pectin preparation, but no gel layer was formed when the CMC preparation and the commercial hydrogel wound dressing were used.
Embodiment 3
[0083] In situ gelation of pectin mediated by calcium ions
[0084] Body fluids such as blood and tears contain calcium ions (8.5-10.3mEq / dl blood). Since aloe pectin forms calcium gels, the role of calcium in the in situ gelation of aloe pectin was examined by using an in vitro gel system to simulate the in situ gel formation with animal serum. This in vitro test method is called the gel positive migration test, wherein, the animal serum is placed at the bottom of the glass tube, and the aloe pectin solution is spread on the top of the serum (according to the density relationship between the test solution and the pectin solution, also The pectin solution can be placed at the bottom of the tube). The formed gel can be distinguished from the pectin solution due to the increased turbidity of the gel formed in the pectin phase when viewed under a light source. Also, if a gel is formed, the interface does not move when the tube is tilted.
[0085] Normal calf serum of tissue cu...
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