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Plant anti-disease agent and its preparation

A yield-increasing agent and plant technology, applied in the fields of botanical equipment and methods, plant growth regulators, animal repellents, etc., can solve the problems of natural environment pollution, endangering people's health, excessive pesticide residues, etc. The effect of improving plant disease resistance

Inactive Publication Date: 2005-02-23
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

Due to the excessive use of chemical fertilizers and chemical pesticides, a series of serious social problems have been caused, which have polluted the natural environment, caused excessive pesticide residues in agricultural products, caused various diseases, and endangered people's health.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0007] The present invention is illustrated by the following examples.

[0008] (1) Preparation of chitosanase fermented liquid: prepare liquid culture medium with following composition and weight ratio: chitosan 2~5% (weight percentage, the same below), glucose 0.1~0.3%, NH 4 NO 3 1~3%, yeast extract 0.3~0.5%, K 2 HPO 4 0.07%, KH 2 PO 4 0.03%, NaCl 0.5%, MgSO 4 ·7H 2 O 0.05%, the rest is water, adjust the pH to 5-7.5, and sterilize. Inoculate chitosanase-producing Vibrio genus (Vibrio Pacini sp) bacteria with an inoculation amount of 5% to 10%, cultivate them for 2 to 4 days at 25°C to 35°C, filter the fermentation broth with a plate frame, and obtain the filtrate as It is a chitosanase fermentation broth.

[0009] The medium composition and weight used in the present embodiment are specifically: chitosan 300g, glucose 20g, NH 4 NO 3 200g, yeast extract 30g, K 2 HPO 4 7g, KH 2 PO 4 3g, NaCl 50g, MgSO 4 ·7H 2 O 5g, water 938.5g, adjust pH6.5.

[0010] (2...

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Abstract

A chemical for the plant to resist against diseases and increase yield is prepared through preparing culture medium from chitosan, glucose, NH4NO3, yeast extract, K2HPO4, KH2PO4, NaCl, MgSO4.7H2O and water, fermentive culture of the bacteria able to generate chitosanase, preparing the fermented liquid containing chitosanase, enzymolizing chitosan to obtain enzymolized product, and proportionally mixing it with composite amino acid solution.

Description

technical field [0001] The invention relates to a plant disease-resistant and yield-increasing agent and a preparation method thereof. Background technique [0002] my country is a large agricultural country. Fertilization in the main grain and vegetable production areas is dominated by chemical fertilizers, mainly including nitrogen, phosphorus, and potassium. The excessive and unreasonable use of chemical fertilizers and the unreasonable proportion of different fertilizer types have caused soil compaction, excessive decomposition of organic matter, reduced soil microbial activity, and intensified plant diseases and insect pests. The control of crop diseases and insect pests generally uses chemical pesticides. Due to the excessive use of chemical fertilizers and chemical pesticides, a series of serious social problems have been caused, which have polluted the natural environment, caused excessive pesticide residues in agricultural products, caused various diseases, and end...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02
Inventor 韩宝芹刘万顺王常红彭燕飞
Owner OCEAN UNIV OF CHINA
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