Preparation of gentian effective part and its application

A technology of effective parts and gentian, applied in the field of preparation of effective parts of traditional Chinese medicine

Inactive Publication Date: 2005-03-09
SHANXI PUDE PHARMA CO LTD
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem solved by this invention is to obtain the effective part of Gentiana with significant hepatoprotective and anti-inflammatory effects on the basis of the pharmacological screening model. The effective part can generally represent the efficacy of traditional medicinal materials, but it has controllable quality. Compared with traditional Chinese patent medicines, the traditional Chinese medicine preparations made from this effective part have advantages in many aspects such as quality controllability and curative effect. significantly improved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation of gentian effective part and its application
  • Preparation of gentian effective part and its application
  • Preparation of gentian effective part and its application

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0067] Example 1, Preparation Example 1 of the effective fraction of Gentiana composed of loganic acid and gentiopicroside

[0068] Take 100g of gentian crude powder, add 400mL of 30% ethanol solution, reflux for 1 hour, repeat the extraction twice, filter out the extract, combine, recover to 100mL under reduced pressure, refrigerate for 1 day, centrifuge, and take the supernatant , added to a chromatographic column equipped with 300mL HP20 filler, rinsed with 500mL deionized water, then rinsed with 60% ethanol solution, collected 600mL of ethanol eluate, evaporated to dryness under reduced pressure, and vacuum dried to obtain 7.6g of the effective fraction of Gentiana.

[0069] The active part of gentian obtained by TLC can be seen to have clear spots at the same Rf value as the standard products loganic acid and gentiopicroside;

[0070] The content of loganic acid and gentiopicroside was determined by HPLC, and the content of the two main compounds accounted for 66.54% of t...

example 2

[0071] Example 2, take 100g of gentian crude powder, add 400mL of 40% acetone solution, reflux for 1 hour, repeat the extraction 3 times, filter the extract, combine, recover to 100mL under reduced pressure, refrigerate for 2 days, centrifuge, take Add the supernatant to a chromatographic column equipped with 300mL HP20 filler, rinse with 500mL deionized water, then rinse with 60% ethanol solution, collect 600mL of ethanol eluate, evaporate to dryness under reduced pressure, and dry in vacuum to obtain the effective fraction of Gentiana 6.2 g.

[0072] The active part of gentian obtained by TLC can be seen to have clear spots at the same Rf value as the standard products loganic acid and gentiopicroside;

[0073] The content of loganic acid and gentiopicroside was determined by HPLC, and the content of the two main compounds accounted for 90.54% of the solid content of the effective part.

example 3

[0074] Example 3, the preparation example of the powder injection of the active part of Gentiana composed of loganic acid and gentiopicroside

[0075] Powder Injection Prescription:

[0076] Active parts of gentian 300g

[0077] Mannitol 300g

[0078] Add water for injection to 4000mL

[0079] Preparation method: According to the above prescription, dissolve the active part of gentiana and mannitol in water for injection, add 4g of activated carbon, boil at 100°C for 15 minutes, and let it stand for 24 hours; filter, add dilute hydrochloric acid to adjust the pH to 7.0; dispense in 10ml of penicillin In a vial, 4 mL each; freeze-dry in a freeze dryer at -40°C for 5 hours; sublimate at -15°C-20°C for a total of 24 hours; dry at 40°C for 2 hours; press cap and seal.

[0080] Properties: This product is a light yellow lump

[0081] Quality index: each preparation contains 300 mg of active part of gentian, and each contains no less than 150 mg of loganic acid and gentiopicrosi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A process for extracting the lognic acid and gentiopicroside from Chinese gentian and their application in preparing medicines for treating liver injury, cholecystitis and bile duct obstruction are disclosed.

Description

technical field [0001] The invention relates to the preparation of an effective part of a traditional Chinese medicine and the application of the effective part in the field of pharmacy. Background technique [0002] At present, liver disease caused by various reasons is still the enemy that threatens human health all over the world. In developing countries, the situation is even more serious, especially the high incidence of various viral hepatitis. Viral hepatitis (viral hepatitis) is a common infectious disease caused by a variety of hepatitis viruses. It is estimated that there are about 350 million hepatitis B virus (HBV) carriers in the world. It is conservatively speculated that HBV carriers account for about 10% of the world's total population, and nearly 1 million people die of diseases caused by HBV every year. China is one of the regions with a high HBV infection rate in the world. [0003] According to the national hepatitis epidemiological survey data from th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K9/08A61K9/14A61K9/16A61K9/20A61K31/7048C07H1/08C07H17/04C07H17/075
Inventor 胡少勇袁解乾
Owner SHANXI PUDE PHARMA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap