Use of protein fingerprint method in detecting protein fingerprint of colon cancer and rectum cancer in blood

A protein and protein technology, applied in the field of protein fingerprinting and protein fingerprinting of biological sample analysis, can solve the problems of inability to detect biomolecules, difficult to distinguish biomolecules, complex and other problems

Inactive Publication Date: 2005-03-16
BEIJING SELDI TECH
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  • Summary
  • Abstract
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  • Claims
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Problems solved by technology

First, the method provides only two characteristics of biomolecules—mass and isoelectric point (pI)
Second, the resolution in each dimension is limited by the resolving power of the gel
For example, it is often difficult to distinguish between biomolecules that differ by less than 5% in mass or that differ in pI
Third, gels have limited capacity a

Method used

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  • Use of protein fingerprint method in detecting protein fingerprint of colon cancer and rectum cancer in blood

Examples

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Embodiment 1

[0063] Example 1 Distinguishing protein fingerprints in blood from normal human cells, cells from patients with colorectal cancer and cells from patients with colorectal polyposis

[0064] (1) Experimental method

[0065] 1. Materials

[0066] 1. Source of specimens: In the colon cancer patient group, there were 146 colorectal cancer patients, all of whom were pathologically diagnosed as colorectal cancer, including 20 cases of Dukes A stage, 126 cases of Dukes B, Dukes C, and Dukes D stages in total. There were 62 cases of normal people in the normal group. Benign colorectal disease control group included 32 patients with benign colorectal disease, including 10 cases of colorectal adenoma, 6 cases of familial polyposis, and 16 cases of colorectal non-neoplastic polyposis. All three groups were between 40 and 60 years old and had similar exposure histories. The male to female ratio in the colon cancer patient group was 97 males and 49 females. Benign colorectal disease gro...

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Abstract

This invention also relates to protein dactylography method for analyzing for the protein group got by retention chromatography. This method adopts mass spectroscopy to distinguish and detect protein group. The analysis data takes the form of bar code (protein dactylography) read by computer. This invention method is used to detect the protein fingerprint in the normal cell, restocarcinoma patient cell and rectal polyp patient cell.

Description

technical field [0001] The invention relates to a novel method for analyzing proteins in biological samples, in particular to a protein fingerprint method for analyzing biological samples. The present invention also relates to protein fingerprinting in which the proteome is captured by retention chromatography and analyzed using a computer readable barcode format (protein fingerprint). Background technique [0002] Both normal function and pathological characteristics of cells depend to some extent on the function of proteins expressed by cells. Therefore, identifying differences in proteins expressed within cells can be used in the diagnosis of disease and ultimately in drug development and disease treatment. However, differential analysis of protein expression and function requires the ability to resolve complex mixtures of intracellular molecules. However, many substances in cells often exist in trace amounts. The current methods for analyzing proteins have limitations ...

Claims

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Application Information

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IPC IPC(8): G01N30/00G01N30/72G01N33/53G01N33/68G06K7/10H01J49/04
Inventor 许洋高春芳
Owner BEIJING SELDI TECH
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