Micro fluidic biological chip based on micro balls

A biochip and microfluidic technology, applied in biological testing, material inspection products, instruments, etc., can solve the problems of lack of different probe molecules, and achieve the goal of promoting miniaturization and automation, increasing surface area, and improving reaction speed Effect

Inactive Publication Date: 2005-03-16
SOUTHEAST UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

However, at present, there are few examples of microfluidic chips being applied to analysis such as nucleic acid hybridization. The reason is that there is a lack of a carrier encoding different probe molecules.

Method used

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  • Micro fluidic biological chip based on micro balls
  • Micro fluidic biological chip based on micro balls
  • Micro fluidic biological chip based on micro balls

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Experimental program
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Effect test

Embodiment Construction

[0014] The present invention is a microfluidic biochip based on microspheres. The biochip is composed of a cover sheet 1 located in the upper half and a substrate 2 located in the lower half; The channel is provided with microspheres of different sizes as probe molecule carriers in the microchannel, and an inlet 16 and an outlet 17 are arranged at both ends of the microchannel. There are at least two steps in the stepped microchannel, and there are at least two types of microspheres of different sizes. Each step corresponds to a kind of microspheres, and the size of the microspheres is used as a code to distinguish different probes fixed on the microspheres. needle molecule. The material of the microsphere as the probe molecular carrier can be glass, polystyrene or rubber, etc., and the size of the microsphere is between 30-100 μm. The length of each step in the stepped microchannel is about 500 μm, the width of the stepped microchannel is 105-205 μm, and the distance between...

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Abstract

It is a micro flow control biological chip based on micro ball, which is used to measure protein and nucleic acid large molecule and can be widely used in the fields of clinic measurement, quarantine inspection, environment monitoring, medicine filter, microbiological assay. The microbiological chip comprises the upper cover 1 and bottom ground 2, wherein, the cover 1 is located with step micro path and the micro path is located with micro balls as probe molecule of different sizes and the both ends of the micro path are located with one entrance 16 and an exit 17. The step micro path has at least two steps and two kinds of micro balls, wherein, one step responds to one micro ball and the size of the micro ball serves as different probe molecules fixed on the micro balls.

Description

technical field [0001] The invention relates to a microsphere-based microfluidic biochip and a preparation method thereof. It can be used to detect biological macromolecules such as proteins and nucleic acids, and can be widely used in clinical testing, inspection and quarantine, environmental monitoring, drug screening, microbial identification, and functional analysis of nucleic acids and proteins. Background technique [0002] Biochip refers to a micro-analysis system constructed on the surface of a square centimeter-sized solid-phase medium through microelectronics and micro-processing technology to achieve rapid, efficient, sensitive processing and processing of DNA, proteins and other biological components in tissues and cells. analyze. It is a biological detection technology developed in the early 1990s, and it is the product of multidisciplinary and comprehensive development. The principle is that in about 1 ~ 2cm 2 Different biomolecular...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N35/00
Inventor 顾忠泽赵祥伟刘兆斌张宏陆祖宏
Owner SOUTHEAST UNIV
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