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Tissue culture technique for peony

A technology of tissue culture and peony, applied in horticulture, botanical equipment and methods, gardening tools/equipment, etc., can solve problems such as not easy to sterilize

Inactive Publication Date: 2005-03-30
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Use calcium hypochlorite 5%-15% or sodium hypochlorite 1%-5% or mercury chloride 0.1% as the basic disinfectant, compound ethanol 70%, Tween 20-80 and water to effectively solve the problem of peony explants. The Sterilization Problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Establishment of aseptic culture system of Yangshan peony cultivar 'Fengdan'

[0011] Fengdan (Paenoia ostti T.Hong et J.X.Zhang var.Lishizhenii B.A.Shenin Acta Phytota.Sin.35(4):360-361.1997), in October-March, collected no pests and diseases, plump scales from healthy plants Buds are used as explants, and the surface is cleaned with detergent, rinsed with running water for 30 minutes in a triangular flask, transferred to an ultra-static workbench, added a mixture of 70% ethanol and 0.15% sodium hypochlorite (1:3), and added dropwise 10 Drop the soil at 80°C, sterilize for 3-11 minutes, rinse with water for 3-4 times, place the washed scale buds in a petri dish, absorb the surface moisture with absorbent paper, peel off the outer scales of the buds with scissors, tweezers, and a scalpel, and inoculate On the pre-prepared medium, cultivate for 3-10 days at 23-28 degrees Celsius, under the condition of 2000-3000 lux light, transfer the uncontaminated culture to the induc...

Embodiment 2

[0013] Tissue Culture and Propagation of Seedlings of the Central Plains Peony Variety 'Ziyaotai'

[0014] Central Plains peony variety 'Zi Sha Tai' (Paeonia suffroticosa T.Hong et J.X.Zhang var.lishizhenii B.A.Shen) collected dormant scale buds in November and established an aseptic culture system according to the method described in Example 1; The body is transferred to the value-added medium for culture, and the value-added medium is the basic medium with cytokinin 6-BA0.3-2 mg / L, auxin NAA 0-3.2 mg / L, at 23-28 degrees Celsius, light 2000 Under the condition of -3000 lux for 28 days, each culture will produce 3-6 adventitious buds. According to the calculation method of tissue culture value-added rate, each adventitious bud can produce 6.7 million tissue culture seedlings per year.

Embodiment 3

[0016] Transplantation of rooted test-tube seedlings of peony variety 'Fengdan' from test tubes to pots in the field

[0017] Fengdan (Paenoia ostti T.Hong et J.X.Zhang var.Lishizhenii B.A.Shen inActa Phytota.Sin.35(4):360-361.1997) rootless tissue culture seedlings were cultivated on the rooting medium for 25 days to produce adventitious roots, forming peony rooting test tubes Seedling. The rooting medium is made by adding 5 mg / liter of cytokinin 6-benzylaminoadenine and 10 mg / liter of auxin indolebutyric acid to the basic medium described in the second point of this manual. The transplanting of rooted test-tube seedlings to the field is carried out in the following three steps: (1) dripping 0.5-5 mg / L of abscisic acid near the terminal buds of the plants to induce dormancy of the scale buds, so that the whole plant enters a dormant state. This step can also be done with 26 The high-temperature treatment of -35 degrees centigrade replaces the chemical reagent treatment for 1...

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PUM

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Abstract

A tissue culture technique for quickly reproducing peony seedlings includes inoculating the explant in a special culture medium, artifically regulating the action intensity of environment factor, and exponentially reproducing the seedlings in artificial culture room by the successive transfer periods (28 days). For one foliage bud, 1.65 millions seedlings can be reproduced in one year.

Description

technical field [0001] The present invention relates to a kind of method for the tissue culture breeding nursery stock of Chinese traditional famous flower peony, specifically, the present invention relates to a kind of surface disinfectant of surface disinfection of peony explant; Proliferation medium for adventitious bud expansion; rooting medium for adventitious bud rooting and seedling growth; inducer for test-tube plantlets to transition from test tube to field conditions; soilless culture substrate for seedlings; and use of surface disinfectants, culture media, transplanting Processes, conditions and steps for breeding ornamental peonies with inducers and soilless culture substrates. Background technique [0002] Peony is a famous traditional Chinese flower. It is honored as the "King of Flowers" because of its grace and luxury. Peonies have been cultivated as ornamental plants since the Southern and Northern Dynasties, and have also spread to Europe, Japan, the Unite...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 罗晓芳王华芳
Owner BEIJING FORESTRY UNIVERSITY
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