Process for screening madicine of antivirus

A screening method and technology for drugs, applied in the fields of biochemical equipment and methods, and the determination/inspection of microorganisms, which can solve the problems of lack of screening methods and lack of screening methods.

Inactive Publication Date: 2005-06-08
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But for most RNA viruses, ideal screening methods are still very lacking
Especially in the development of drugs against plant viruses and fungal virus diseases, there is currently no simple, fast and efficient screening method

Method used

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  • Process for screening madicine of antivirus
  • Process for screening madicine of antivirus
  • Process for screening madicine of antivirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Acyclovir (100 mg / tablet) was prepared into a 160 mg / ml solution with sterilized water, and the insoluble part was removed by centrifugation. The drug was added into the PDA solid medium according to the gradient of 32mg / ml, 3.2mg / ml, and 0.32mg / ml. Inoculate EP721 and culture at room temperature for 7 days. The result shows that 3.2mg / ml drug concentration makes the bacterium colony of fungus obviously become orange ( figure 2 -A), close to the level when there is no virus, indicating that the symptoms have been significantly relieved. And when the drug concentration of 32mg / ml, except that bacterial colony turns orange, its growth has been obviously inhibited.

Embodiment 2

[0034] Quick gram (amantadine hydrochloride 100mg / grain), get 4 capsules and be mixed with the solution of 10ml with sterile water, centrifuge to remove insoluble matter. Drugs were added to the PDA solid medium according to three concentration gradients of 160, 320, and 640ug / ml. Inoculate EP721 and culture at room temperature for 7 days. The result shows that 320ug / ml drug concentration makes the bacterial colony of fungus obviously turn into orange ( image 3 -B), while at the drug concentration of 640ug / ml, except that the bacterial colonies turned orange, their growth was significantly inhibited.

Embodiment 3

[0036] Morpholine guanidine hydrochloride (100mg / tablet), get 100 tablets and dissolve with 50ml sterile water, and centrifuge to remove insoluble matter. Drugs were added into PDA solid medium according to three gradients of 90ug / ml, 900ug / ml, and 9000ug / ml, inoculated with EP721, and incubated at room temperature for 7 days. The results showed that the drug dosage of 900ug / ml made the colony of the fungus obviously turn orange, which was close to the level when there was no virus, indicating that the symptoms had been obviously alleviated. And on the plate of 9000ug / ml, except that bacterial colony becomes orange, its growth has been obviously inhibited ( Figure 4 ). Further extraction of dsRNA verification, with the increase of drug concentration, the concentration of dsRAN decreased slightly ( Figure 5 ).

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Abstract

A method for screening antivirus medicine is based on a test system composed of dual-chain RNA virus and its natural host (chestnut blight bacterium). The chestnut blight bacterium EP-721(-) without virus shows orange color and the chestnut blight bacterium carrying virus CHV1-EP721 shows white color. After the latter is treated by an antivirus medicine, the effect of said medicine can be determined according to the color variation.

Description

technical field [0001] The invention belongs to the technical field of antiviral drug screening. In particular, it relates to a screening method for anti-RNA virus drugs. Background technique [0002] Drug screening is a crucial link in the research and development of new drugs. Simple and effective screening methods can greatly shorten the process of new drug development. Routine drug screening begins with cell plaque experiments, and enters clinical trials after evaluating the effectiveness and toxicity of animal models. Modern drug screening has added methods such as cytopathic inhibition experiments, in vitro reaction inhibition experiments, etc. [1-3] . Each method has a special design basis for a specific virus, and each has advantages and disadvantages. For example, the advantage of in vitro reaction inhibition experiment is that it is fast, but it is necessary to obtain virus and host-specific factors and establish a suitable test system in advance. [4-14] , and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18
Inventor 陈保善林海燕戴璐
Owner GUANGXI UNIV
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