Kelp breeding method based on gamete selection technology
A selection technology, a large-scale technology, applied in the field of seaweed breeding based on the hermaphrodite seaweed gamete selection technology, can solve the problems of low breeding efficiency, poor compatibility, and long breeding cycle, so as to improve breeding efficiency, improve technical level, and shorten breeding time Effect
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[0008] Hereinafter, the cultivation of high-temperature-resistant kelp varieties is taken as an example to further illustrate the present invention.
[0009] The experimental material in this example is kelp gametophyte, which is grown into female and male gametophytes in the laboratory after seedlings are collected from mature kelp sporophytes.
[0010] In this embodiment, the high temperature resistance trait is used as the breeding purpose. The key is to confirm the overlapping expression of kelp heat-tolerant genes in the generations of gametophytes and sporophytes.
[0011] (1) Temperature selection
[0012] Use semi-lethal temperature (18-21℃) as selective pressure to cultivate female and male gametophytes for 12-14 days, select the surviving gametophytes, use growth and development regulation technology to promote their synchronous maturity, and cultivate kelp juvenile sporophytes.
[0013] The cultivated juvenile sporophyte was subjected to marine aquaculture experiments. ...
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