Kelp breeding method based on gamete selection technology

A selection technology, a large-scale technology, applied in the field of seaweed breeding based on the hermaphrodite seaweed gamete selection technology, can solve the problems of low breeding efficiency, poor compatibility, and long breeding cycle, so as to improve breeding efficiency, improve technical level, and shorten breeding time Effect

Inactive Publication Date: 2005-06-29
OCEAN UNIV OF CHINA
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Problems solved by technology

Its breeding work is mainly a process of late discovery and transformation. For the early stage, that is, the laboratory research stage, it is impossible to make any prediction and inference about the characteristics of the variety based on the technical means of laboratory analysis, and it is impossible to identify whether the experimentally improved algae plants are compatible with The purpose of the research is the same

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Embodiment Construction

[0008] Hereinafter, the cultivation of high-temperature-resistant kelp varieties is taken as an example to further illustrate the present invention.

[0009] The experimental material in this example is kelp gametophyte, which is grown into female and male gametophytes in the laboratory after seedlings are collected from mature kelp sporophytes.

[0010] In this embodiment, the high temperature resistance trait is used as the breeding purpose. The key is to confirm the overlapping expression of kelp heat-tolerant genes in the generations of gametophytes and sporophytes.

[0011] (1) Temperature selection

[0012] Use semi-lethal temperature (18-21℃) as selective pressure to cultivate female and male gametophytes for 12-14 days, select the surviving gametophytes, use growth and development regulation technology to promote their synchronous maturity, and cultivate kelp juvenile sporophytes.

[0013] The cultivated juvenile sporophyte was subjected to marine aquaculture experiments. ...

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Abstract

The invention provides a kelp breeding method based on gamete selection technology, which consists of utilizing the superimposition of bisexual reproduction large-scale sea weed sporinite and gamete generation gene expression, selecting gamete (including gametophyte) generation according to breeding destination, thus screening gamete fitting breeding destination, then cultivating sporophyte meeting breeding purpose by utilizing screened gamete in combination with the algae culturing technique. The invention is characterized by the organic integration of laboratory research and sea experiment, wherein the sea cultivation experiment procedure is reduced, which can increase breeding destination and efficiency substantially.

Description

Technical field [0001] The invention relates to an algae breeding method, in particular to a seaweed breeding method based on the technology of gamete selection of bisexual reproductive seaweed. Background technique [0002] Due to the large algae of large economic algae, the complete life history cannot be reproduced in the laboratory. Therefore, breeding research is mainly based on the study of its relatively small gametophytes, through selfing, hybridization, or parthenogenesis. The seedlings are cultivated by developmental methods, and their traits are observed through marine aquaculture experiments. Based on this, combined with the technical methods of biology and genetics to carry out directional genetic improvement work, and cultivate algae varieties that can be used in aquaculture production. The breeding work is mainly a process of later discovery and transformation. For the early stage, that is, the laboratory research stage, it is impossible to predict and infer any va...

Claims

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Application Information

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IPC IPC(8): A01H13/00
Inventor 刘涛崔竞进王翔宇
Owner OCEAN UNIV OF CHINA
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