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Construction and expression of recombinant human trypsinogen 2 expression vector, preparation and application of its monoclonal antibody

A trypsinogen and monoclonal antibody technology, applied in the field of bioengineering, can solve problems such as elevated amylase

Inactive Publication Date: 2005-08-17
FUDAN UNIV
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Problems solved by technology

At present, the level of amylase is usually used to diagnose pancreatitis, but the enzyme activity is not necessarily consistent with the severity of the disease. In mild cases, it may be very high, but it is normal in hemorrhagic necrotizing pancreatitis, and many extrapancreatic diseases can also increase amylase. high

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  • Construction and expression of recombinant human trypsinogen 2 expression vector, preparation and application of its monoclonal antibody
  • Construction and expression of recombinant human trypsinogen 2 expression vector, preparation and application of its monoclonal antibody
  • Construction and expression of recombinant human trypsinogen 2 expression vector, preparation and application of its monoclonal antibody

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Embodiment Construction

[0016] The following examples will help those of ordinary skill in the art to further understand the present invention, but do not limit the present invention in any form.

[0017] 1. Obtaining the target gene and constructing the cloning vector containing the target gene

[0018] Using the human pancreatic phage cDNA library as a template, use the 5'-end primer 5'-ACA CCA TGA ATC TAC T(C / T)CTGA-3' and the 3'-end primer 5'-GTA TAG AGA CTG CAG AGG G-3' Carry out PCR amplification of human trypsinogen-2 gene, and the amplified product is subjected to 1% agarose electrophoresis, which shows a specific amplification band with a size of about 750bp, which is consistent with the expected 778bp. All PCR amplifications showed specific bands. The amplified Tg2 fragment was gel-cut, purified and quantified, then ligated with pGEM-T vector (promega company product) to construct pGEM-Tg2, and then transformed into Escherichia coli JM-109. The size of the transformant was identified by t...

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Abstract

The present invention belongs to the field of gene engineering technology, and is especially one kind of recombinant plasmid vector containing human trypsinogen gene 2(hTg-2). The present invention also discloses the constitution and expression of the expression vector and the preparation of the monoclonal antibody. The monoclonal antibody has high specificity and high stability, and may be used in preparing reagent and medicine for the diagnosis, treatment and prevention of diseases related with the abnormal expression of hTg-2 protein.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and specifically relates to a plasmid expression vector and human trypsinogen 2 protein expressed by the vector. The present invention also relates to the application of the protein in the preparation of monoclonal antibodies and their application in diagnosis, treatment or prevention of related diseases. Background technique [0002] Human trypsinogen 1 and 2 are both composed of 247 amino acids, of which amino acid residues 1 to 15 are signal peptides, and amino acids 16 to 23 are activation peptides that are truncated when the zymogen is activated into trypsin (Trypsinogen activation peptide, TAP). Ectopic activation of trypsinogen is the initial stage of acute pancreatitis. In recent years, the incidence of severe pancreatitis has gradually increased, the mortality rate is about 20%, and those with complications can be as high as 50%. In emergency conditions, rapid diagnosis of acut...

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Application Information

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IPC IPC(8): A61K39/395C07K16/40C12N5/12C12N9/50C12N15/57C12N15/63C12N15/64C12N15/70G01N33/53
Inventor 朱乃硕涂艳欧西军
Owner FUDAN UNIV
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