Collagen and method for preparing collagen from skins of birds

A collagen and protoprotein technology, which is applied in the field of type I poultry skin collagen, can solve the problems of limited application of collagen and low degree of collagen purification, and achieves the effects of simple and easy procedure, simple method and low preparation cost.

Inactive Publication Date: 2005-10-26
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the low degree of purification of collagen

Method used

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  • Collagen and method for preparing collagen from skins of birds

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: preparation of quail skin collagen

[0024] 1. Wash the quail skin. Soak and rinse the frozen quail skin with 100ml of 4°C tap water, remove the quail hair and subcutaneous tissue until there is no fluff in the soaking solution except the whole piece of quail skin, then soak and rinse with deionized water for 5 times.

[0025] 2. Alkali treatment of quail skin. Use 100ml of 0.1M NaOH solution to soak the quail skin after (1) treatment at 4°C for 2-3 days, and replace the 0.1M NaOH solution once a day. Then fully wash the quail skin after alkali treatment with deionized water until about pH7.

[0026] 3. Acid soluble collagen. Dissolve the treated quail skin in 40ml of 0.5M acetic acid-sodium acetate (1:1 / V:V) buffer solution, extract continuously for 3 days at 4°C, and filter with gauze. The residue was acid-dissolved and extracted again, and the steps were the same as above, and the two filtrates were combined.

[0027] 4. Purify co...

Embodiment 2

[0029] Embodiment 2: preparation of quail skin collagen

[0030] 1. Wash the quail skin. Soak and rinse the frozen quail skin with 100ml of tap water at 1-10℃, remove the quail hair and subcutaneous tissue until there is no fluff in the soaking liquid except the whole piece of quail skin, then soak and rinse with deionized water for 5 times .

[0031] 2. Alkali treatment of quail skin. with 3% Ca(OH) 2 Soak (1) treated quail skin in 100ml of solution at 1-10°C for 3-4 days, replace with 3% Ca(OH) once in the morning and evening 2 solution. Then fully wash the quail skin after alkali treatment with deionized water until about pH7.

[0032] 3. Acid soluble collagen. Dissolve the treated quail skin in 40ml of 0.5M acetic acid-sodium acetate (1:1 / V:V) buffer solution, extract continuously for 3 days at 4°C, and filter with gauze. The residue was acid-dissolved and extracted again, and the steps were the same as above, and the two filtrates were combined.

[003...

Embodiment 3

[0034] Embodiment 3: preparation of salted chicken skin collagen

[0035] 1. Wash the chicken skin. Soak and rinse the frozen chicken skin with 200ml of 5°C tap water, remove chicken feathers and subcutaneous tissue until there is no fluff in the soaking solution except the whole piece of chicken skin, then soak and rinse with deionized water for 5 times.

[0036] 2. Alkali treatment of chicken skin. The chicken skin was salted with NaCl (1:1 w / w) for 7 days, and then the alkali-treated chicken skin was fully washed with deionized water until the pH was about 7.

[0037] 3. Acid soluble collagen. Dissolve the chicken skin treated in (2) in 100ml of 0.5M acetic acid-sodium acetate (1:1 / V:V) buffer solution, extract at 4°C for 3-4 days, and filter with gauze. The residue was acid-dissolved and extracted again, and the steps were the same as above, and the two filtrates were combined.

[0038] 4. Purify collagen. The above filtrate was centrifuged at 10,000 r...

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Abstract

The present invention discloses a collagen prepared by utilizing fowls kin as raw material, and the prepared fowl skin collagen is formed from alpha and beta chains. Said preparation method included the following steps: cleaning fowl skin, soaking the cleaned fowl skin in alkaline solution, then using deionized water to wash the alkali-treated fowl skin, soaking the above-mentioned fowl skin in acidic buffer liquor, filtering, centrifugalizing, collecting supernatant and dialyzing untie the dialyzate is made into neutrality. Said invention contains no gamma chain, its alpha chain content is high and beta chain content is low.

Description

technical field [0001] The invention relates to a collagen for poultry tissue engineering and a preparation method. Using poultry skin as a raw material, type I collagen is extracted. Specifically, the present invention relates to treating the cleaned poultry skin with an alkaline solution, and then treating the skin with an acid-soluble method, and purifying the acid-soluble supernatant to obtain type I poultry skin collagen. Background technique [0002] Collagen is a structural protein that has a variety of biological functions. Collagen is the support and filler of tissues, actively participates in cell migration, differentiation and proliferation, and is related to wound repair and embryonic development. Biological substitutes prepared from collagen have achieved good results in animal experiments and clinical experiments. [0003] At present, a number of biological products with collagen as the main component have appeared in the world, including...

Claims

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Application Information

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IPC IPC(8): C07K14/78
Inventor 钱卫平陆璐董健
Owner SOUTHEAST UNIV
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