Sterilizing liquid for quick inactivating AIDS sex desease virus and sperm and its preparing method

Abstract

The present invention provides a kind of sterilizing liquid capable of inactivating HIV, sex disease pathogens and sperm. The sterilizing liquid consists of chlorhexidine acetate 0.1-4.5 weight portions, polyoctyl phenol oxyethylene ether 0.01-6 weight portions, hyaluronic acid 0.01-8 weight portions, dihydroxymethyl glycolylurea 0.1-3 weight portions, aloe 25-60 weight portions, and pure water 30-70 weight portions. It may also contain nipagin M 0.1-2 weight portions, lactic acid 0.05-2 weight portions, glycerol 0.1-5 weight portions and hydroxyethyl cellulose 0.1-3 weight portions. The present invention also provides the preparation process of the sterilizing liquid.

Description

technical field

[0001] The invention relates to the field of medicines and disinfection supplies, in particular to a bactericidal liquid capable of rapidly inactivating HIV, various venereal disease pathogens and sperm and a preparation method thereof. Background technique

[0002] Currently, the incidence of various sexually transmitted diseases is increasing dramatically every year at an alarming rate. By the end of 2002, the number of people living with HIV in China had exceeded 1 million. China has entered a period of rapid growth of AIDS, and it is estimated that by 2010, the number of HIV-infected persons will reach 10 million. AIDS threatens every one of us and every family. As an extremely urgent public health and social problem, the impact of AIDS has penetrated into all social strata, accompanied by tests of science, economy, morality, ethics and civilization, as well as the impact on human life and Dignity challenge. The rapid development of AIDS clearly shows...

Examples

Embodiment 1

[0039] Example 1 Preparation of bactericidal solution

[0040] (1) According to the formula of each experimental scheme in Table 1, respectively get the raw materials of the corresponding weight, and mix them to obtain a mixture:

[0041] raw material

[0042] (2) according to the prescription of each experimental scheme in table 2, take the raw material of corresponding weight portion respectively, join in the mixture prepared by step (1), reconcile and stir into transparent hydrogel:

[0043] raw material

[0044] (3) The filling machine is divided into finished products.

Embodiment 2

[0045] The bactericidal effect experiment of embodiment 2 bactericidal liquid

[0046] 1. Experimental materials

[0047] Sample: the bactericidal liquid prepared in Example 1, a pale yellow transparent liquid, stored at room temperature.

[0048] Test strains: Leptospira jaundice hemorrhagic type 56601 Leptospira water flu typhoid type 56609 was used for the killing test of spirochetes;

[0049] 2. Neutralizer test:

[0050] 2.1 Inspection basis: refer to 2.4 of the first volume of the "Technical Specification for Disinfection" of the Ministry of Health.

[0051] 2.2 The neutralizer identification test adopts the suspension quantitative method, the action time of the bactericidal liquid and the test strain is 1 minute respectively, and the neutralization time is 10 minutes. respectively at 37°C CO 2 Neisseria gonorrhoeae culture in an incubator and Leptospira culture in a 28°C incubator. The experiment was repeated 5 times.

[0052] 3. Quantitative sterilization test: ...

Embodiment 3

[0066] Embodiment 3 sterilization liquid is to the killing effect experiment of HIV

[0067] 1. Test basis: "Virus inactivation test" in paragraph 2.1.1.10 of "Technical Specifications for Disinfection" (2002 edition).

[0068] 2. Experimental materials:

[0069] 2.1 Sample: the sterilizing solution prepared in Example 1, stored at room temperature.

[0070] 2.2 Human immunodeficiency virus: HIV~1 IIIB strain, passaged and cultured in MT4 cells.

[0071] 2.3 Cells: MT4 cells.

[0072] 2.4 Cell culture medium: RPMI 1640 culture medium containing 10 calf serum.

[0073] 2.5 Sterile deionized water.

[0074] 2.6 96-well cell culture plate.

[0075] 3. Experimental method:

[0076] Before the experiment, the sterilizing liquid samples were prepared into solutions of 1:10, 1:20, and 1:50 with sterile deionized water. Mix 0.9ml of samples with different concentrations (1:10, 1:20, 1:50) with 0.1ml of HIV-1 suspension, put them at room temperature for 1 minute, 5 minutes, and ...

technical field

[0001] The invention relates to the field of medicines and disinfection supplies, in particular to a bactericidal liquid capable of rapidly inactivating HIV, various venereal disease pathogens and sperm and a preparation method thereof. Background technique

[0002] Currently, the incidence of various sexually transmitted diseases is increasing dramatically every year at an alarming rate. By the end of 2002, the number of people living with HIV in China had exceeded 1 million. China has entered a period of rapid growth of AIDS, and it is estimated that by 2010, the number of HIV-infected persons will reach 10 million. AIDS threatens every one of us and every family. As an extremely urgent public health and social problem, the impact of AIDS has penetrated into all social strata, accompanied by tests of science, economy, morality, ethics and civilization, as well as the impact on human life and Dignity challenge. The rapid development of AIDS clearly shows...

Examples

Embodiment 1

[0039] Example 1 Preparation of bactericidal solution

[0040] (1) According to the formula of each experimental scheme in Table 1, respectively get the raw materials of the corresponding weight, and mix them to obtain a mixture:

[0041] raw material

[0042] (2) according to the prescription of each experimental scheme in table 2, take the raw material of corresponding weight portion respectively, join in the mixture prepared by step (1), reconcile and stir into transparent hydrogel:

[0043] raw material

[0044] (3) The filling machine is divided into finished products.

Embodiment 2

[0045] The bactericidal effect experiment of embodiment 2 bactericidal liquid

[0046] 1. Experimental materials

[0047] Sample: the bactericidal liquid prepared in Example 1, a pale yellow transparent liquid, stored at room temperature.

[0048] Test strains: Leptospira jaundice hemorrhagic type 56601 Leptospira water flu typhoid type 56609 was used for the killing test of spirochetes;

[0049] 2. Neutralizer test:

[0050] 2.1 Inspection basis: refer to 2.4 of the first volume of the "Technical Specification for Disinfection" of the Ministry of Health.

[0051] 2.2 The neutralizer identification test adopts the suspension quantitative method, the action time of the bactericidal liquid and the test strain is 1 minute respectively, and the neutralization time is 10 minutes. respectively at 37°C CO 2 Neisseria gonorrhoeae culture in an incubator and Leptospira culture in a 28°C incubator. The experiment was repeated 5 times.

[0052] 3. Quantitative sterilization test: ...

Embodiment 3

[0066] Embodiment 3 sterilization liquid is to the killing effect experiment of HIV

[0067] 1. Test basis: "Virus inactivation test" in paragraph 2.1.1.10 of "Technical Specifications for Disinfection" (2002 edition).

[0068] 2. Experimental materials:

[0069] 2.1 Sample: the sterilizing solution prepared in Example 1, stored at room temperature.

[0070] 2.2 Human immunodeficiency virus: HIV~1 IIIB strain, passaged and cultured in MT4 cells.

[0071] 2.3 Cells: MT4 cells.

[0072] 2.4 Cell culture medium: RPMI 1640 culture medium containing 10 calf serum.

[0073] 2.5 Sterile deionized water.

[0074] 2.6 96-well cell culture plate.

[0075] 3. Experimental method:

[0076] Before the experiment, the sterilizing liquid samples were prepared into solutions of 1:10, 1:20, and 1:50 with sterile deionized water. Mix 0.9ml of samples with different concentrations (1:10, 1:20, 1:50) with 0.1ml of HIV-1 suspension, put them at room temperature for 1 minute, 5 minutes, and ...