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Method for preserving and proliferating jujube witches broom pytoplasma in vitro of host

A technology for jujube mad disease and phytoplasma, which is applied in the field of sustainable preservation and multiplication of jujube mad disease phytoplasma, and can solve the problems of failure to establish a technical system for jujube tree tissue culture, difficulty in inoculating successfully, and easy death, etc. problem, to achieve the effect of rapid proliferation and long-term preservation

Inactive Publication Date: 2006-02-08
HEBEI AGRICULTURAL UNIV.
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Problems solved by technology

However, due to the strong lethality of jujube madness, the jujube tree hormone disorder after the disease, the growth is weak, it is difficult to inoculate successfully in tissue culture, and it is easy to die
[0004] So far, the tissue culture technology system for jujube trees suffering from jujube madness has not been established

Method used

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  • Method for preserving and proliferating jujube witches broom pytoplasma in vitro of host

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Embodiment Construction

[0020] Selection and disinfection of explants: Take Fuping jujube plants suffering from jujube madness as test materials, collect 1-2 year sick branches that have shown symptoms of jujube madness in the dormant season, and carry out hydroponics indoors, and use diseased branches for hydroponics The buds that go out are cultivated as explants, and the explants are rinsed with running water for 30 minutes with 0.12% HgCl 2 Sterilize for 8 minutes, then rinse with distilled water for 5 times; select MS medium as the basic medium, containing 3% sucrose, 0.45% agar, and the pH of the medium before sterilization is 6.0; the light cycle of the culture room is 15 hours of light and 9 hours of darkness , the light intensity is 1400-1600Lux, and the temperature is controlled at 27±2°C; inoculate the sterilized Jujube madiasis explants in hormone-free MS medium for start-up culture, and the tissue culture seedlings grow vigorously, differentiate rapidly, and exhibit Typical symptoms of j...

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Abstract

Disclosed is a method for in vivo preservation and propagation of jujube witches broom phytoplasma in exsomatized hosts through jujube tree tissues suffering from the disease, which comprises explant selecting, sterilizing, start up culturing, enrichment culturing and rooting culturing.

Description

technical field [0001] The invention relates to a method for sustainable preservation and multiplication of jujube mad disease phytoplasma. Background technique [0002] At present, at least 300 kinds of plants are known to be harmed by phytoplasma. Since phytoplasma cannot be artificially isolated and cultivated, it is difficult to carry out research on its nutrition, physical and chemical properties, and pathogenic mechanism, which seriously hinders the development of phytoplasma diseases. Research progress, its prevention is an international problem. [0003] Jujube is an important characteristic and dominant fruit tree in China. Jujube madness, the most serious and common disease in jujube production, is a typical fatal phytoplasma disease. For a long time, in order to improve research efficiency and overcome this disease as soon as possible, people try to set up a kind of indoor research platform always. Since there has been no breakthrough in the artificial isolatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 刘孟军赵锦周俊义
Owner HEBEI AGRICULTURAL UNIV.
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