Method for selectively breeding bacterial wilt-resistant variety of tomato

A technology for resisting R. solanacearum and R. solanacearum, which is applied in the field of breeding tomato varieties resistant to R. solanacearum, can solve the problems of difficult breeding, complex genetic background of tomato bacterial wilt, and no public reports on breeding, so as to shorten generations and time , saving breeding materials, saving manpower and material resources

Inactive Publication Date: 2006-02-22
ZHEJIANG UNIV
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AI Technical Summary

Problems solved by technology

The complex genetic background of tomato bacterial wilt brings difficulties to breeding
Although some research has been done on the mappi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Sow homozygous tomato seeds on plug trays, and inoculate at the 3-4 leaf stage with a root-cutting method at a concentration of 4*10 8 10 strains of R. solanacearum bacteria liquid per ml were not inoculated. 7-16 days after inoculation, fresh tomato leaves were taken to extract enzymes according to the method in (XUE YinglongOUYANG Guangcha, AO Shaogen. Studies on the plant phenlanine ammonielyase [J]. Acta Phytophysioloca Sinica. 1983, 9 (3): 301-305) Liquid, carry out the determination of phenylalanine ammonia lyase (PAL), use (ZHU Guanglian, ZHONGHaiwen, ZHANG Haiqin.A Laboratory Mannal of plantPhysiology[M].Beijing: Science Press, 1990:51-54.in china) The method of extracting superoxide dismutase (SOD) and measuring it. Measure every other day, usually 5-8 times, and compare the enzyme activity of inoculated tomato and non-inoculated tomato. The peak value of PAL and SOD of inoculated tomato is more than 40% and 15% higher than that of non-inoculated tomato, which...

Embodiment 2

[0018] Sow homozygous tomato seeds on plug trays, and inoculate at the 3-4 leaf stage with a root-cutting method at a concentration of 4*10 810 strains of R. solanacearum bacteria liquid per ml were not inoculated. 7-16 days after inoculation, fresh tomato leaves were taken to extract enzymes according to the method in (XUE YinglongOUYANG Guangcha, AO Shaogen. Studies on the plant phenlanine ammonielyase [J]. Acta Phytophysioloca Sinica. 1983, 9 (3): 301-305) Liquid, carry out the determination of phenylalanine ammonia lyase (PAL), with (ZHU Guanglian, ZHONGHaiwen, ZHANG Haiqin.A Laboratory Mannal of plantPhysiology[M].Beijing: Science Press, 1990:51-54.(in china) ) method to extract superoxide dismutase (SOD) and measure. Measure every other day, usually 5-8 times, and compare the enzyme activity of inoculated tomato and non-inoculated tomato. The peak value of PAL and SOD of inoculated tomato is more than 40% and 15% higher than that of non-inoculated tomato, which can be s...

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PUM

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Abstract

The invention provides a method for detecting the tomato seed with wilt-resistance, which makes use of the marking of the feature of resistant enzyme changing after inoculating the wilt with the length polymorphism gene molecule in the enlarged enzyme cut DNA fragment, and can get the breed or parental generation with tomato wilt-resistant gene. The method of this invention possesses the following characteristics: only small quantity seed is needed for the breeding of seed with wilt-resistance, the resistant difference of large quantity homozygous tomato seeds or strains can be analyzed, and choose out the resistant breeding material; the breeding generation and time can be shortened, a plurality of strain can be analyzed at the same time, the resistant gene can be identified within 2-3 months when there is the parental generation and F2 seed, which allows the new seed breeding to be finished in 1-2 years and saves labor and material resources greatly.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a method for measuring and breeding varieties using gene molecular markers and resistant enzymes, in particular to the combination of using AFLP (amplified enzyme cleavage DNA fragment length polymorphism) molecular markers and resistant enzyme determination indicators, A method for breeding tomato varieties resistant to bacterial wilt. Background technique [0002] Ralstia solanacearum is the bacterium Ralstia solanacearum Smith, which was named Pseudomonas solanacearum in 1896 by Eruim Smith of the United States. It has two sub-classification systems, which are divided into 4 races (physiological races) according to different host ranges, and Race I is harmful to tomato, potato, eggplant and other Solanaceae crops and tobacco. The second is to divide it into 5 Biovars (biochemical variants) according to the sugar and acid utilization of lactose, maltose, cellobiose, mannose,...

Claims

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Application Information

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IPC IPC(8): A01H1/00A01H3/00
Inventor 寿森炎冯壮志卢钢杨悦俭苗立祥王汉荣黄锡志
Owner ZHEJIANG UNIV
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