Saccharide composition with protection on microecologic prepn
A probiotic and protective technology, applied in the field of medicine, can solve the problems of limited promotion and application, increased circulation cost, low bacterial survival rate, etc., and can reduce blood cholesterol and triglyceride, inhibit tumor occurrence, and enhance body immunity. effect of function
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Embodiment 1
[0033] Take 0.01 g of hyaluronic acid and 1 g of trehalose, dissolve and mix them with deionized water, set the volume to 10 ml, filter and sterilize with a 0.22 μm microporous membrane to obtain the protective agent of the present invention. Take 0.5 mL of the above protective agent, weigh 0.1 mg of the wet bacterial cells after centrifugation, and suspend in the protective agent. Add a certain amount of bifidofactors that have been sterilized in advance as needed to obtain a mixed bacterial solution.
[0034] The mixed bacteria solution was divided into sterile glass bottles with stoppers, pre-frozen to about -35°C, and then placed in a freeze dryer for about 25 hours to freeze-dry to obtain freeze-dried bacteria powder. Residual moisture in the product is required to reach 2% to 3%.
Embodiment 2
[0036] Take 0.01 g of hyaluronic acid and 1 g of trehalose, dissolve and mix them with deionized water, set the volume to 10 ml, filter and sterilize with a 0.22 μm microporous membrane to obtain the protective agent of the present invention. Take 0.5 mL of the above protective agent, weigh 0.1 mg of the wet bacterial cells after centrifugation, and suspend in the protective agent. Add a certain amount of bifidofactors that have been sterilized in advance as needed to obtain a mixed bacterial solution.
[0037] Pack the mixed bacteria solution into sterile glass bottles with stoppers, put them in a vacuum dryer at a vacuum degree of 20-30 Pa and a product temperature of 30°C for about 48 hours to obtain dried bacteria powder. Residual moisture in the product is required to reach 2% to 3%.
Embodiment 3
[0039] Take 0.01 g of hyaluronic acid and 1 g of trehalose, dissolve and mix them with deionized water, set the volume to 10 ml, filter and sterilize with a 0.22 μm microporous membrane to obtain the protective agent of the present invention. Take 0.5 mL of the above protective agent, weigh 0.1 mg of the wet bacterial cells after centrifugation, and suspend in the protective agent. Add a certain amount of bifidofactors that have been sterilized in advance as needed to obtain a mixed bacterial solution.
[0040] The mixed bacteria solution was divided into aseptic stoppered glass bottles, and spray-dried in a centrifugal spray dryer with an inlet temperature of 120°C and an outlet temperature of 80°C to obtain dry bacteria powder. Residual moisture in the product is required to reach 2% to 3%.
[0041] Experimental research data of the present invention
[0042] In this experiment, the bacterial solution without protective agent was freeze-dried in the same way as the negativ...
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