Preparation of effective mycoenamine with microbial lytic acrose and its derivative

A technology for acarbose and derivatives, which is applied in the field of preparing effective memtenamine, can solve the problems of no industrialized production, low yield, complex lysate components, etc., and achieves low production cost, huge economic benefits, and good application prospects. Effect

Active Publication Date: 2006-03-01
ZHEJIANG UNIV OF TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] ②Prepared by NBS (N-Bromosuccinimide) reagent cleavage method, using NBS reagent, can break the C-N bond in effective mycosuccinimide or its derivatives to obtain effective mycosuccinimide, and also generate some cyclohexanone substances, lysate The composition is relatively complex, and effective mycylamine can be separated by ion exchange and other methods, but there is no report on industrial production
[0013] (3) Preparation by chemical synthesis method. Since effective mycylamine was isolated in 1972, there have been many methods for chemically synthesizing effective mycylamine, but most of the chemically synthesized ones are racemates, and the yield is low
[0016] So far, there is no report on the field of microbial decomposition of acarbose and its derivatives to produce effective mycylamine in domestic and foreign literature

Method used

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  • Preparation of effective mycoenamine with microbial lytic acrose and its derivative
  • Preparation of effective mycoenamine with microbial lytic acrose and its derivative
  • Preparation of effective mycoenamine with microbial lytic acrose and its derivative

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Fermentation medium formula (weight / volume percentage, the same below): Acarbose 2.0%, (NH 4 ) 2 SO 4 1.5%, KCl 0.5%, Na 2 HPO 4 12H 2 O 1.0%, MgSO 4 0.02%, prepared with tap water, adjusted to pH 6.0, and set aside.

[0038] Take 150mL of the above-mentioned culture medium, evenly distribute it in three 250mL Erlenmeyer flasks, and sterilize. Inoculate the slant strain CCTCC No.M 205091, cultivate the bacteria, shake the rotating speed of 200r / min, and cultivate it at 30°C for 48 hours as the seed liquid, and set aside.

[0039] Take 2L of the above culture medium, divide it into 20 500mL shake flasks, and sterilize. The seed solution was inserted, the inoculum amount was 5% (v / v), and culture was carried out at a culture temperature of 28° C., a shaker speed of 200 r / min, and a fermentation time of 120 h.

[0040] Collect 1.8L of the above-mentioned fermentation broth, centrifuge to remove the bacteria, and put the supernatant on the column (D113 resin, NH 4...

Embodiment 2

[0042] Fermentation medium formula: acarbose 1.0%, (NH 4 ) 2 SO 4 0.5%, KCl 0.1%, Na 2 HPO 4 12H 2 O 0.1%, MgSO 4 0.02%, prepared with tap water, adjusted to pH 7.0, and set aside.

[0043] Take 150mL of the above-mentioned seed culture medium, evenly distribute it in three 250mL Erlenmeyer flasks, and sterilize. Insert the slant strain CCTCC No.M205091, cultivate the bacteria, shake the rotating speed of 150r / min, and cultivate it at 28°C for 48 hours as the seed solution, and set aside.

[0044] Take 2L of the above culture medium, divide it into 20 500mL shake flasks, and sterilize. The seed solution was inserted, the inoculum amount was 5% (v / v), and culture was carried out, the culture temperature was 30° C., the rotation speed of the shaker was 200 r / min, and the fermentation time was 72 hours.

[0045] 1.8 L of the above-mentioned fermentation broth was collected, separated and purified, and the steps were the same as in Example 1 to obtain 1.1 g of effective ...

Embodiment 3

[0047] Fermentation medium formula: acarbose 2.5%, (NH 4 ) 2 SO 4 2.0%, KCl 1.0%, Na 2 HPO 4 12H 2 O 2.0%, MgSO 4 0.1%, prepared with tap water, adjusted to pH 8.0, and set aside.

[0048] Take 150mL of the above-mentioned culture medium, evenly distribute it in three 250mL Erlenmeyer flasks, and sterilize. Inoculate the slant strain CCTCC No.M 205091, cultivate the bacteria, shake the rotating speed of 200r / min, and cultivate in the shaking table at 32°C for 48h as the seed liquid, and set aside.

[0049] Take 2L of the above culture medium, divide it into 20 500mL shake flasks, and sterilize. The seed solution was inserted, the inoculum amount was 5% (v / v), and culture was carried out, the culture temperature was 32° C., and the shaker speed was 200 r / min. The fermentation time is 144h.

[0050] 1.8 L of the above-mentioned fermentation broth was collected, separated and purified, and the steps were the same as in Example 1 to obtain 3.0 g of effective mycylamine. ...

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Abstract

This invention discloses a new kind of microorganism which has the ability to lysis Acarbose and/or its derivatives to produce validamycoenamine. This microorganism belongs to Klebsiellaí»s Klebsiella oxytoca CCTCC No.M 205091. This invention discloses method of producing validamycoenamine using this microorganism to lysis Acarbose and/or its derivatives. The microorganism CCTCC No.M 205091 directly ferments and decomposes substrates Acarbose and/or its derivatives by culturing in the medium. Or get the thallus by culturing in the medium and produce validamycoenamine by catalyzing decomposing the substrates Acarbose and/or its derivatives.

Description

technical field [0001] The present invention relates to a new microorganism screened and isolated from soil, and also relates to the preparation of effective valienamine by using the new microorganism to decompose acarbose (acarbose) and its acarbose derivatives (acarbose derivatives) Methods. Background technique [0002] The valienamine involved in the present invention is called valienamine in English, and its structural formula is as follows: (Chem. Rev. 2003, 103: 1955-1977). [0003] [0004] Effective mycylamine, also known as Jinggang mycylamine, its chemical name: (1S, 2S, 3S, 4R)-1-amino-5-(hydroxymethyl)cyclohex-5-ene-2,3,4- Trihydric alcohol; molecular formula is C 7 h 13 NO 4 , the important groups are: a primary amino group (-NH 2 ), a carbon-carbon double bond (C=C), a hydroxymethyl group (-CH 2 OH), three hydroxyl groups (-OH). Its hydrochloride (C 7 h 13 NO 4 · HCl)[a] D 23 For +68.6 ° (1N-HCl), pentaacetate (C 17 h ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00C12R1/22
Inventor 郑裕国薛亚平王远山陈小龙沈寅初
Owner ZHEJIANG UNIV OF TECH
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