Microbial culture medium and use thereof
A technology for microbial culture medium and culture medium, applied in the field of microbial culture medium, can solve the problems of poor accuracy of the sensitivity test method of sterile test medium, no CFU counting method of Clostridium sporogenes, large errors and the like.
Inactive Publication Date: 2006-03-15
NAT INST FOR THE CONTROL OF PHARMA & BIOLOGICAL PROD
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Problems solved by technology
[0015] Existing studies have proved that the accuracy of the sensitivity test method of the sterility test medium in the "Procedures" is not good (see Gao Shangxian et al. "A preliminary study on the accuracy of the sensitivity test method of the sterility test medium", "Journal of Pharmaceutical Analysis ", 2004, 24(1): 52-58)
However, in the Pharmacopoeia, the turbidimetric dilution method is used to prepare the bacterial suspension, and the error is large, so it is difficult to accurately add the number of viable bacteria of the quality control bacteria.
The CFU count of quality control bacteria disclosed in the 2005 edition of "Pharmacopia" is a dilution method, not a real CFU count, and the CFU count of Clostridium sporogenes lacks operability
There is no CFU counting method for Clostridium sporogenes in foreign countries
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment 1
[0067] Add 15.0g of tryptone, 5.0g of yeast extract powder, 2.5g of sodium chloride, 0.5g of L-cystine, 0.5g of sodium thioglycolic acid or 0.5g of thioglycolic acid, 8.0 of glucose, and 5g of agar into 1000ml of water, mix and dissolve at low temperature , adjust the pH to be weakly alkaline, boil, filter, add 5.0 g of glucose, shake well, adjust the pH value to 7.1 ± 0.2 after sterilization, sterilize, subpackage, and the agar concentration is 0.5%.
Embodiment 2
[0069] With embodiment 1, difference is, agar is 6g, and agar concentration is 0.6%.
Embodiment 3
[0071] With embodiment 1, difference is, agar is 7g, and agar concentration is 0.7%.
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Abstract
A microbial medium and its use are disclosed. The medium consists of pancreatic casein peptolysis 5-30 proportion, yeast extractive powder 0.5-20 proportion, grape sugar 0.5-20 proportion, sodium chloride 1-15 proportion, L-cystine 0.1-5 proportion and sulfur sodium ethylate or sulfur glycolic acid 0.1-8 proportion. It is characterized by adding agar into medium with content=50-500g / cm2. It can be used for CFU count of anaerobe or facultative anaerobe.
Description
field of invention [0001] The present invention relates to a kind of microorganism culture medium and its application, especially a kind of improved thioglycolate culture medium, this culture medium is suitable for the colony forming unit (Colony Forming Units, CFU) of anaerobic bacteria or facultative anaerobic bacteria Counting, especially Clostridium sporogenes (abbreviated as Clostridium sporogenes) colony forming units (ColonyForming Units, CFU) counting, and used for sterility test medium sensitivity test, belongs to the field of biological products. Background technique [0002] As an important supporting condition for scientific research and applied science, microbial culture medium (referred to as culture medium) plays an irreplaceable role by other means. Unless otherwise specified, drugs, biological products, dressings, sutures, sterile instruments, and varieties requiring sterility testing in the Pharmacopoeia all require sterility testing. Sterility test medium...
Claims
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IPC IPC(8): C12N1/00C12N1/32C12N1/20
Inventor 高尚先孙彬裕康国华
Owner NAT INST FOR THE CONTROL OF PHARMA & BIOLOGICAL PROD
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