Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of microorganism fermentation liquid for alfalfa ensilage

A technology of microbial fermentation liquid and fermentation liquid, which is applied in the field of preparation of microbial fermentation liquid, and can solve problems such as affecting fermentation and reducing the pH value of silage

Inactive Publication Date: 2006-04-12
SHANGHAI JIAO TONG UNIV
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in addition to a variety of lactic acid bacteria, the green juice fermentation broth also contains a variety of harmful bacterial species. These bacteria also have the role of initiating factors, inducing the expansion of harmful bacterial populations in the process of alfalfa silage, thereby reducing or slowing down The green juice fermented liquid can better promote the expansion and reproduction of lactic acid bacteria, so that the pH value of the silage can not be sharply reduced in a short period of time during the pre-fermentation period and the early acidification stage of the silage alfalfa. This is the green juice fermented liquid used as a silage additive in alfalfa silage The biggest defect that affects the high-quality fermentation of silage alfalfa and the formation of high-quality silage
In further searches, there is no report on mixing green juice fermented liquid and streptococcus faecalis as an alfalfa silage additive and its preparation method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Select fresh alfalfa materials: take fresh alfalfa plants, remove about 10cm from the base and the top, remove the main branches and thicker branches at the same time, and keep the remaining young branches and leaves as the production material of green juice fermentation liquid;

[0019] 2. Make green juice fermentation liquid: chop the fresh alfalfa material into 3-5cm, add distilled water according to the ratio of alfalfa (g) and water (ml) 1:2.5, mix well, mash with a high-speed tissue grinder, statically Place for 1 hour, filter with double-layer gauze, add glucose or syrup with an equivalent amount of glucose in the filtrate according to the ratio of alfalfa raw material: glucose=10g:1g, fully stir, place 30 DEG C for fermentation for 48 hours, and make green juice fermentation liquid;

[0020] 3. Activation of Streptococcus faecalis: Put Streptococcus faecalis in MRS liquid medium for activation, the activation temperature is 35°C, and the activation time is 24 ...

Embodiment 2

[0026] 1. Select fresh alfalfa materials: take fresh alfalfa plants, remove about 10cm from the base and the top, remove the main branches and thicker branches at the same time, and keep the remaining young branches and leaves as the production material of green juice fermentation liquid;

[0027] 2. Make green juice fermentation liquid: chop the fresh alfalfa material into 3-5cm, add distilled water according to the ratio of alfalfa (g) and water (ml) 1: 2.5, mix well, mash with a high-speed tissue grinder, Stand still for 1 hour, filter with double-layer gauze, add glucose or syrup corresponding to the amount of glucose to the filtrate according to the ratio of alfalfa raw material: glucose = 10g: 1g, fully stir, and place it at 30 ° C for 48 hours to ferment to make green juice fermentation liquid ;

[0028] 3. Activation of Streptococcus faecalis: Put Streptococcus faecalis in MRS liquid medium for activation, the activation temperature is 37°C, and the activation time is ...

Embodiment 3

[0034] 1. Select fresh alfalfa materials: take fresh alfalfa plants, remove about 10cm from the base and the top, remove the main branches and thicker branches at the same time, and keep the remaining young branches and leaves as the production material of green juice fermentation liquid;

[0035] 2. Make green juice fermentation liquid: chop the fresh alfalfa material into 3-5cm, add distilled water according to the ratio of alfalfa (g) and water (ml) 1: 2.5, mix well, mash with a high-speed tissue grinder, Stand still for 1 hour, filter with double-layer gauze, add glucose or syrup corresponding to the amount of glucose to the filtrate according to the ratio of alfalfa raw material: glucose = 10g: 1g, fully stir, and place it at 30 ° C for 48 hours to ferment to make green juice fermentation liquid ;

[0036] 3. Activation of Streptococcus faecalis: Put Streptococcus faecalis in MRS liquid medium for activation, the activation temperature is 37°C, and the activation time is ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A microbiological fermented liquid for preparing the ensilage of alfalfa is composed of the fermented liquid of green juice and the liquid culture medium MRS containing faecal streptococcus. Its preparing process includes such steps as choosing fresh alfalfa, preparing the fermented liquid of its green juice, activating faecal streptococcus, and proportional mixing. It can decrease the pH value of ensilage and increase its quality.

Description

technical field [0001] The invention relates to a method in the field of biotechnology, in particular to a method for preparing alfalfa silage microbial fermentation liquid. Background technique [0002] Alfalfa (Medicago sativa L., hereinafter referred to as alfalfa) is a perennial leguminous forage with rich nutritional value. It is a high-quality forage for herbivores and has the reputation of "the king of forage". For a long time, alfalfa has been preserved mainly by sun-dried hay. The biggest disadvantage of this method is the serious loss of nutrients. In recent years, Canada, Japan and other countries have begun to use the method of silage to preserve alfalfa, which has solved the problem of serious loss of nutrients in the process of alfalfa preservation. Because alfalfa has low soluble sugar content and high buffer energy, it is a legume forage that is difficult to silage, and the direct silage effect is not good, so it is necessary to use additives in the silage f...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K3/03C12R1/46A23K30/18
Inventor 安渊田瑞霞王光文
Owner SHANGHAI JIAO TONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com