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Production of 3-hydroxy-propylaldehyde from klebsiella by glycerin aerobic fermentation

A technology of Klebsiella and hydroxypropanal, applied in the field of 3-hydroxypropanal production, can solve the problem of unknown mechanism of action

Inactive Publication Date: 2006-05-31
HUAQIAO UNIVERSITY
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Problems solved by technology

Slininger et al added semicarbazide hydrochloride to glycerol to achieve the extracellular accumulation of 3-HPA, but the specific mechanism of action is unknown

Method used

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Embodiment Construction

[0012] Utilize Klebsiella pneumoniae DSM 2026 to transform the method for the aerobic fermentation of glycerol to prepare 3-hydroxypropionaldehyde (3-HPA), comprising: inoculating Klebsiella in a phosphate buffer fermentation medium containing semicarbazide hydrochloride and glycerol The bacterium Klebsiella pneumoniae DSM 2026 produces 3-hydroxypropanal under aerobic conditions.

[0013] A preferred production method, comprising:

[0014] (1) 250mL seed medium (containing glycerol, 20.0g / L; K 2 HPO 4 , 3.4g / L; KH 2 PO 4 , 1.3g / L; (NH 4 ) 2 SO 4 , 2.0g / L; MgSO 4 ·7H 2 O, 0.2g / L; yeast extract, 1.0g / L; CaCO 3 , 2.0g / L; FeSO 4 ·7H 2 O, 5.0×10 -3 g / L; CaCl 2 , 2.0×10 -3 g / L; trace elements, 2.0g / L) were placed in a 500mL reaction vessel, and 1mL was activated and stored in LB medium (containing tryptone, 10.0g / L; yeast extract, 5.0g / L; NaCl , 10.0g / L, pH7.0) containing Klebsiella pneumoniae DSM 2026 bacteria solution, cultured in a rotary shaker at 120r / min for 12h...

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Abstract

The invention provides a method that seeding klebsiella pneumoniae DSM 2026 in the phosphate-buffers fermention substrate with semicarbazide hydrochloride and glycerol, and can produce 3- hydroxy propaldehyde under aerobic condition. The condition of optimizing isú‘the cultivating time of seed liquid is 12 hours, Inoculation Quantity of thalli is 2.16g(cell dryweight) / L, the glycerol content is 30g / L, the semicarbazide hydrochloride content is 26.8g / L, fermenting 27 hours under 28 deg.c and the ph is equal 7.2, it can get the ferment liquid of 3- hydroxy propaldehyde. It can mensurate that the concentration of 3-HPA in the ferment liquid is 15.48g / L, the total gain rate and conversion rate are 0.60g / g, 75%.

Description

technical field [0001] The invention relates to a production method of 3-hydroxypropanal, in particular to a method for producing 3-hydroxypropanal by converting glycerol under aerobic conditions through Klebsiella Klebsiellapneumoniae DSM 2026. Background technique [0002] 3-Hydroxypropanal (3-HPA) has high application value in chemical industry. It is a potent antimicrobial agent and acts as a food preservative or therapeutic aid. It is also the precursor of many industrial organic compounds such as acrylic acid, acrolein, 1,3-propanediol, etc. 3-HPA can be chemically oxidized to produce acrylic acid. Acrylic acid is generally obtained from petroleum processing and can be used to produce important polymer monomers of acrylates, polyacrylic acid and its salts. 3-HPA hydrogenation catalysis can produce 1,3-propylene glycol, which is an important monomer for the production of new polyesters (such as PTT), polyethers and polyurethanes. The production and application of PTT ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/24C12R1/22
Inventor 方柏山陈宏文
Owner HUAQIAO UNIVERSITY
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