Paddy rice tip flower dereloped new gene Dhl and its clone method

Abstract

The invention relates to a rice glumous fower growing new gene Dh1 and its cloning method. It belongs to rice function gene analytic technique field. Endonuclease Hind III is used to cut Japanese fine corresponding BAC. After electrophoresis object DNA segment is recycled. It is connected to the carrier cutting with the same enzyme to gain one gene Dh1, and used complementary assay to verify its function. The GFP fusion expression carrier is used to study its space-time pattern of manifestation. It lays a foundation for study rice new species in future.

Description

technical field [0001] The invention relates to the technical field of functional gene analysis of rice. Background technique [0002] T-DNA tagging is a high-throughput method for cloning and analyzing plant functional genes. In Arabidopsis and other higher plants, T-DNA tagging technology has been widely used to clarify gene functions, and a number of Genes (Jeon et al., 2001; Tyagi et al., 2000; Krysan et al., 1999 et al.; Green et al., 1999; Papi et al., 2000; Oyama et al., 2002). With the establishment of the efficient Agrobacterium-mediated T-DNA rice transformation technology system (Heli et al., 1994), there have also been constructions of T-DNA insertion mutant libraries and successful use of the system to isolate and clone genes in rice. reported (Jung et al., 2003; Jeon et al., 2000; Jeong et al., 2002; Wu et al., 2003; Lee et al., 2004). [0003] Although the full sequence of rice genes has been known to humans, the roles of each gene fragment in the growth and...

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Problems solved by technology

[0003] Although the full sequence of the rice gene has been known to humans, the roles of each gene segment in the growth and generation of rice are still unknown

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