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Process for determining content of zinc ion by enzyme method and kit for diagnosing zine ion thereof

A diagnostic kit and zinc ion technology, applied in the field of medical testing and determination, can solve the problems affecting the use and promotion, the difference in the accuracy of the sensitivity test results, etc., and achieve low test costs, reliable test results, and complete three-dimensional space structure. Effect

Inactive Publication Date: 2006-06-14
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with different detection reagents and testing routes, the sensitivity of detection and the accuracy of detection results will be very different, which directly affects the use and promotion of this method. This is why people continue to research, develop and innovate in this field. the reason for

Method used

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  • Process for determining content of zinc ion by enzyme method and kit for diagnosing zine ion thereof
  • Process for determining content of zinc ion by enzyme method and kit for diagnosing zine ion thereof
  • Process for determining content of zinc ion by enzyme method and kit for diagnosing zine ion thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment one (single agent)

[0037] Prepare the zinc ion diagnostic kit according to the following ingredients and dosage:

[0038] Glycine buffer 80mmol / l,

[0039] 4-nitrophenol phosphate 5mmol / l,

[0040] Alkaline phosphatase 14000U / l,

[0041] Ethylene glycol 50% (accounting for the total volume of the reagent).

[0042] Set on the automatic biochemical analyzer (Hitachi-7080): temperature 37°C, reaction time 10 minutes, test main wavelength 405nm, test secondary wavelength 505nm or more, the volume ratio of the tested zinc ion sample to the reagent is 1:25, The reaction direction is positive reaction (absorbance rises, the same below).

[0043] Add the sample and the prepared single dose, and the two are automatically mixed inside the analyzer to detect and record the increase of the absorbance at 405nm. According to the rate method, end point method and other methods, the content of zinc ions in the sample is measured and calculated accor...

Embodiment 2

[0044] Embodiment two (one of double dosage example)

[0045] Prepare the zinc ion diagnostic kit according to the following ingredients and dosage:

[0046] Reagent I——

[0047] Imidazole ~ hydrochloric acid buffer 100mmol / l,

[0048] 4-nitrophenol phosphate 10mmol / l,

[0049] Glycerol 50% (accounting for the total volume of reagent I);

[0050] Reagent II——

[0051] Imidazole ~ hydrochloric acid buffer 100mmol / l,

[0052] Alkaline phosphatase 20000U / l,

[0053] Ethylene glycol 50% (accounting for the total volume of reagent II).

[0054] Set on the automatic biochemical analyzer (Hitachi-7080): temperature 30°C, reaction time 15 minutes, test main wavelength 405nm, test secondary wavelength 505nm or more, the total volume of the measured zinc ion sample and reagent I and reagent II The ratio is 1:25, the ratio of the amount of reagent I to reagent II is 4:1, and the reaction direction is positive reaction.

[0055] Add the sample and rea...

Embodiment 3

[0056] Embodiment three (two of double-dose example)

[0057] Prepare the zinc ion diagnostic kit according to the following ingredients and dosage:

[0058] Reagent I——

[0059] Imidazole ~ hydrochloric acid buffer 100mmol / l,

[0060] Alkaline phosphatase 25000U / l,

[0061] Glycerol 50% (accounting for the total volume of reagent I);

[0062] Reagent II——

[0063] Imidazole ~ hydrochloric acid buffer 100mmol / l,

[0064] 4-nitrophenol phosphate 12mmol / l,

[0065] Propylene glycol 20mmol / l.

[0066] Set on the automatic biochemical analyzer (Hitachi-7080): temperature 37°C, reaction time 10 minutes, test main wavelength 405nm, test secondary wavelength 505nm or more, the total volume of the measured zinc ion sample and reagent I and reagent II The ratio is 1:25, the ratio of the amount of reagent I to reagent II is 4:1, and the reaction direction is positive reaction.

[0067] Add the sample and reagent I first, then add reagent II after 5 ...

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Abstract

The invention relates to enzyme method measuring zinc ion concentration and zinc ion diagnose kit. The invention uses alkaline phosphatase reaction colorimetry method that uses alkaline phosphatase to enzymolysis four-nitrophenol phosphoric acid to form into four-nitrophenol under the activation of zinc ion, by detecting the ascending speed of 405nm dominant wavelength absorbance the zinc ionic content in sample would be quantitatively responsed. The method has high specificity, has no influence from inside and outside metal material, accurate test result. The diagnose kit is made up into bi-agent or tri-agent that could reduce cross influence from each component and keeps stability of the agent that is convenient for long-term storage. The method could quickly test on commonness ultraviolet / visible light, analyzer or semi-transfer / full automatic biochemical study instrument, and doesn't need particularity or extra instrument. The testing cost is cheap, and is convenient for popularization.

Description

technical field [0001] The invention relates to a method for measuring the content of zinc ions in samples such as serum and plasma by enzymatic method, and a zinc ion diagnostic kit prepared by applying the method, which belongs to the technical field of medical inspection and measurement. Background technique [0002] Zinc element is related to the growth and development of organisms, and has important physiological functions, nutritional functions and clinical diagnosis and treatment significance. Determination of zinc ion content and Cu in serum 2+ / Zn 2+ The ratio has important practical value for evaluating the nutritional status of the body's zinc element and for diagnosing and treating many diseases. [0003] The determination methods of zinc ions in the prior art include: complexometric titration, fluorescence photometry, colorimetry, polarography, atomic absorption spectrophotometry, anodic stripping voltammetry and neutron activation method. Complexometric titr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/75G01N21/31
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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