Process for determining content of zinc ion by enzyme method and kit for diagnosing zine ion thereof

A diagnostic kit and zinc ion technology, applied in the field of medical testing and determination, can solve the problems affecting the use and promotion, the difference in the accuracy of the sensitivity test results, etc., and achieve low test costs, reliable test results, and complete three-dimensional space structure. Effect
CN1786695AInactive Publication Date: 2006-06-14SUZHOU ANJ BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUZHOU ANJ BIOTECHNOLOGY CO LTD
Publication Date
2006-06-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to enzyme method measuring zinc ion concentration and zinc ion diagnose kit. The invention uses alkaline phosphatase reaction colorimetry method that uses alkaline phosphatase to enzymolysis four-nitrophenol phosphoric acid to form into four-nitrophenol under the activation of zinc ion, by detecting the ascending speed of 405nm dominant wavelength absorbance the zinc ionic content in sample would be quantitatively responsed. The method has high specificity, has no influence from inside and outside metal material, accurate test result. The diagnose kit is made up into bi-agent or tri-agent that could reduce cross influence from each component and keeps stability of the agent that is convenient for long-term storage. The method could quickly test on commonness ultraviolet / visible light, analyzer or semi-transfer / full automatic biochemical study instrument, and doesn't need particularity or extra instrument. The testing cost is cheap, and is convenient for popularization.
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Description

technical field

[0001] The invention relates to a method for measuring the content of zinc ions in samples such as serum and plasma by enzymatic method, and a zinc ion diagnostic kit prepared by applying the method, which belongs to the technical field of medical inspection and measurement. Background technique

[0002] Zinc element is related to the growth and development of organisms, and has important physiological functions, nutritional functions and clinical diagnosis and treatment significance. Determination of zinc ion content and Cu in serum 2+ / Zn 2+ The ratio has important practical value for evaluating the nutritional status of the body's zinc element and for diagnosing and treating many diseases.

[0003] The determination methods of zinc ions in the prior art include: complexometric titration, fluorescence photometry, colorimetry, polarography, atomic absorption spectrophotometry, anodic stripping voltammetry and neutron activation method. Complexometric titr...

Claims

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