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Process for deprotein in refinishing polyose

A deproteinization and polysaccharide technology, applied in the field of deproteinization and polysaccharide refining, can solve the problems of unsatisfactory protein removal rate, hidden danger of product safety and low efficiency, and achieve the effects of low cost, stable process and good repetition rate.

Inactive Publication Date: 2006-06-28
天津市林业果树研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sometimes the removal rate of protein is not ideal
And protease, as a biological agent, is expensive, resulting in a higher cost of the product
The Sevag method has a high protein removal rate, but it needs to use a large amount of organic solvents in the removal process, and the process is complicated, which not only brings hidden dangers to product safety, but also has low efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: first crush the fruit body of Ganoderma lucidum, and extract the obtained water extract through water extraction, cool the obtained water extract, and lower the temperature of the water extract to 0°C. Then add citric acid to the water extract to adjust the pH of the feed solution to a pH of 4.2; stir and mix evenly, and act for 2 hours at a temperature of 0-5°C. Then the feed liquid is put into a centrifuge for centrifugation, the centrifuge speed is 1000rpm / min, the centrifugation time is 10 minutes, and the supernatant is extracted after centrifugation. Add NaOH to the supernatant to adjust the pH to 7.0. Concentrate the feed solution and dry to obtain the deproteinized Ganoderma lucidum polysaccharide.

Embodiment 2

[0012] Example 2: Lower the temperature of the lentinan water extract to 5°C. Then add malic acid into the water extract to adjust the pH value of the feed solution to reach a pH value of 4.0; stir and mix evenly, and act at this temperature for 1 hour. Then the feed liquid is put into a centrifuge for centrifugation, the centrifuge speed is 2000rpm / min, the centrifugation time is 10 minutes, and the supernatant is extracted after centrifugation. Add NaOH to the supernatant to adjust the pH to 7.0. Concentrate the feed solution and dry to obtain protein-depleted lentinan.

Embodiment 3

[0013] Example 3: The temperature of the astragalus polysaccharide water extract was lowered to 3°C. Then add citric acid to the water extract to adjust the pH of the feed solution to a pH value of 4.2; stir and mix evenly, and act at this temperature for 1.5 hours. Then the feed liquid is put into a centrifuge for centrifugation, the centrifuge speed is 1000rpm / min, the centrifugation time is 10 minutes, and the supernatant is extracted after centrifugation. Add NaOH to the supernatant to adjust the pH to 7.0. Concentrate the feed solution and dry to obtain protein-depleted astragalus polysaccharide.

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PUM

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Abstract

The invention relates to a deproteinization method in polysaccharide refine manufacture. Decreasing the temperature of plant or fungus polysaccharide water extract, adding organic acid, adjusting the pH value to 3.5-4.5, mixing equal, reacting for 1-2 hours, taking centrifugal separation, using the supernatant, adjusting the pH value to 7.0, concentrating the liquid , drying, the polysaccharide that has been deproteinized would be gained. The invention has high efficiency, low cost, simple technology, high recurrence rate, etc.

Description

technical field [0001] The invention belongs to a method for extracting and processing plant active ingredients, in particular to a deproteinization method in polysaccharide refining. Background technique [0002] At present, in the production and refining of plant and fungal polysaccharides, the commonly used deproteinization methods are protease method and Sevag method. Protease method, due to the specificity of the enzyme, makes the protein removal rate vary due to different raw materials, and its effect is unstable. Sometimes the removal rate of protein is not ideal. And protease, as a biological agent, is expensive, resulting in a higher cost of the product. The Sevag method has a high protein removal rate, but it needs to use a large amount of organic solvents in the removal process, and the process is complicated, which not only brings hidden dangers to product safety, but also has low efficiency. Contents of the invention [0003] The technical problem to be sol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 刘建华张志军李淑芳王小琼杨丽维王文治
Owner 天津市林业果树研究所
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