Preparation method of polysaccharides from sea cucumber

A sea cucumber polysaccharide and sea cucumber technology, which is applied in the directions of antitoxin, blood diseases, extracellular fluid diseases, etc., can solve the problems of low product yield and purity, large amount of organic solvent, low purity, etc., so as to avoid the decrease of sea cucumber polysaccharide content. , The reaction conditions are easy to control, and the protein removal rate is high.

Inactive Publication Date: 2011-04-06
HAINAN JINXING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Although there are patents and documents related to the extraction process of sea cucumber polysaccharides at present, they all have the following disadvantages: 1. The production cycle is long, such as the Chinese patent application CN101451157A, which requires at least 28 hours; 2. The yield and purity of the product are low, and the yield is not as good as 1%, the purity is also lower than 80%; 3. The amount of organic solvent used is large, and a large amount of chloroform is used, which not only pollutes the environment, but also affects the final product

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  • Preparation method of polysaccharides from sea cucumber

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Embodiment 1

[0020] Grind 1.0kg of dried sea cucumber, add 2L of 2% sodium hydroxide solution twice the weight, stir and extract at room temperature for 2 hours, extract 2 times in total, filter, and combine to obtain 3.82L filtrate, cool the filtrate to room temperature, adjust When the pH value reaches 8.0, add 20ml of 2% trypsin (enzyme solution concentration 1mg / ml) and stir well, enzymolyze at 40°C for 2 hours, then adjust the pH value of the enzymolysis solution to 4.0 with 1% hydrochloric acid, and then add 2 % pepsin 20mL (enzyme solution concentration 1mg / ml), after enzymatic hydrolysis at 40°C for 2h, and then enzymatic passivation in boiling water at 100°C for 5min. After the enzymatic hydrolysis, use trichloroacetic acid to remove protein, add 10% trichloroacetic acid 10ml directly to the enzymolysis solution to adjust the pH to 3.0, and stir while adding to produce a precipitate, then place it at 4°C for 4 hours, and centrifuge to take the supernatant Solution 3.06L, add 2 tim...

Embodiment 2

[0022] Mince 1.5 kg of fresh sea cucumber, add 4 times the weight of 2% sodium hydroxide solution, stir and extract at room temperature for 3 hours, and filter; add 3 times the weight of 2% sodium hydroxide solution to the filter residue, stir and extract at room temperature for 4 hours, filter; combine twice Filtrate, get 4.13L filtrate, filtrate is cooled to room temperature, adjust pH value to 7.2 by adjusting 0.5% sulfuric acid solution, add 1% trypsin 30ml (enzyme solution concentration 1.5mg / ml) fully stir, at 36 ℃ enzymolysis 3 After 1 hour, adjust the pH value of the enzymolysis solution to 5.0 with 0.5% sulfuric acid, then add 30mL of 1.5% pepsin (enzyme solution concentration 1.4mg / ml), enzymolysis at 42°C for 1h, and then carry out in boiling water at 100°C Enzymatic passivation for 15 minutes. After the enzymolysis is complete, use trichloroacetic acid to remove protein, add about 15ml of 10% trichloroacetic acid directly to the enzymolysis solution to adjust the p...

Embodiment 3

[0024] Mince 2.0 kg of fresh sea cucumber, add 3 times the weight of 2% sodium hydroxide solution, stir and extract at room temperature for 4 hours, and filter; add 4 times the weight of 2% sodium hydroxide solution to the filter residue, stir and extract at room temperature for 2 hours, filter; combine twice Filtrate, get 5.25L filtrate, filtrate is cooled to room temperature, adjust pH value to 9.0 by adjusting 1% hydrochloric acid solution, add 3% trypsin 20ml (enzyme solution concentration 1.5mg / ml) fully stir, enzymolysis 4 at 50 ℃ After 1 hour, adjust the pH value of the enzymolysis solution to 6.0 with 1% hydrochloric acid, then add 20mL of 3% pepsin (enzyme solution concentration 1.4mg / ml), enzymolysis at 50°C for 1h, and then carry out in boiling water at 100°C Enzymatic passivation for 10 minutes. After the enzymolysis is complete, use trichloroacetic acid to remove protein, add about 15ml of 10% trichloroacetic acid directly to the enzymolysis solution to adjust the...

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Abstract

The invention discloses a preparation method of polysaccharides from sea cucumber, which comprises the following steps: (1) extraction: using 2% sodium hydroxide solution to extract fresh sea cucumber or dried sea cucumber; (2) enzymolysis: adopting two-step enzymolysis process with trypsinase and pepsin for carrying out enzymolysis on extraction solution; and (3) deproteinization: adopting trichloroacetic acid for deproteinization and further using high-concentration ethanol for recrystallization of the polysaccharides from the sea cucumber. The yield of the prepared polysaccharides from the sea cucumber is 11.27% on average, the purity is 98.4% on average, the production cycle is about 19 hours, and the prepared product is greatly improved in comparison with the existing process.

Description

technical field [0001] The invention relates to the technical field of health products, in particular to a preparation method of sea cucumber polysaccharide. Background technique [0002] Sea cucumber belongs to Echinodermata, sea cucumber class. Sea cucumber, also known as sea cucumber, is widely distributed in the South China Sea and the Yellow Sea. Sea cucumber has a long history as a health-preserving food. According to the "Compendium of Materia Medica Supplements", sea cucumber is sweet in taste, nourishes the kidney meridian, relieves urination, strengthens yang and treats wilting. Current studies have found that sea cucumbers are rich in nutrients, with a protein content of 52.2%, and sea cucumber polysaccharides and other active substances account for about 15%, mainly mucopolysaccharides and sea cucumber glycosaminoglycans, which have anti-cancer, anti-cancer, immune-enhancing, anti-thrombosis, and anti-aging properties. , anti-virus and other effects. [0003] ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P35/00A61P37/04A61P7/02A61P39/00A61P31/12
Inventor 展学孔周海妹刘全胜
Owner HAINAN JINXING PHARMA
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