Method for extracting collagen comprising microbial fermentation
A collagen, microbial technology, applied in animal/human protein, fermentation, peptide/protein composition, etc., can solve problems such as fragility
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0061] Example 1 Identification of Bacterial Strains of Collagen Extracted by Bacterial Culture Fermentation
[0062] [055] Bacteria were collected and used to extract collagen from the collagen-containing tissue described above by fermentation.
[0063] These bacteria were identified as bacterial strain TW-S-7-1. When preparing bacterial cultures for identification, bacterial strains are picked onto nutrient agar plates. The plates were then placed at 37°C for 16 hours. Five bacterial monoclones were then inoculated into five different "L" tubes containing 5 ml of fresh nutrient broth. Place the test tube at 37° C., shake (50-200 rpm) and incubate for 16-24 hours. Another tube containing 5 ml of fresh nutrient broth not inoculated with any bacteria served as a negative control. Control tubes were incubated at 37°C with shaking (50-200 rpm).
[0064] [056] The turbidity of 5 test tubes inoculated with bacteria was measured to indicate the process of microbial proliferatio...
Embodiment 2
[0076] Example 2 Production of collagen from avian tissue by bacterial fermentation
[0077] [064] About 800 grams of chicken feet were washed with tap water. About 400 grams of soft tissue, including meat, skin and tendon (commonly referred to as tissue), was collected from these materials and cut into small pieces (0.5 cm in length and width). These tissues were soaked in 70% ethanol for 15 min and then air dried in a biosafety cabinet for 0.5–2 h.
[0078] [065] A fermentation process was performed using a Gram-positive bacterial strain belonging to the genus Bacillus to extract collagen from tissue. The microorganism was cultured in a nutrient medium at 37° C. with shaking at 150 rpm for 24 hours. One ring (about 10 pl) of fresh bacterial culture was inoculated into four Erlenmeyer flasks with 125 moi hinton containing 25 mi of nutrient medium, and cultured with shaking at 250 rpm for 24 hours. The contents of each Erlenmeyer flask were scaled up to 100 ml and incubated...
Embodiment 3
[0087] Example 3 Extraction of Collagen from Pig Tissue by Bacterial Fermentation
[0088] [073] The skin of freshly slaughtered pigs was first washed with double distilled water, followed by a second washing process to remove impurities. The washing process is combined with 0.2N NaOH and 0.01-0.2% hypochlorite aqueous solution. The incubation time for each treatment ranged from 15 minutes to 40 minutes. After incubation, the treated pigskin was cut into small pieces (approximately 0.5 cm in length and width), followed by two consecutive thorough washings with hot water and air drying.
[0089] [074] Fermentation was performed using a Gram-positive bacterial strain belonging to the genus Bacillus to extract collagen from pigskin. Bacterial cultures were grown in 25 ml nutrient broth at 37°C for 24 hours on a shaker at 150 rpm. It was then scaled up to 100ml and transferred to a fermenter for 24 hours.
[0090] Stirring and aeration rates were set at 450 rpm and 3 wm, respe...
PUM
| Property | Measurement | Unit |
|---|---|---|
| molecular weight | aaaaa | aaaaa |
| molecular weight | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More