Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for producing chlamydospore by trichoderma liquid deep fermentation

A technology for chlamydospore and Trichoderma, which is applied in the directions of botanical equipment and methods, fungi, biocides, etc., can solve the problems of long fermentation cycle, high fermentation cost, low fermentation yield, etc. The effect of cost reduction and fermentation cost reduction

Active Publication Date: 2006-08-23
HAINAN JINYUFENG BIOLOGICAL ENG
View PDF0 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the fermentation cycle is too long, the fermentation yield is low, and the fermentation cost is high, there are no reports of chlamydospore preparations at home and abroad.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing chlamydospore by trichoderma liquid deep fermentation
  • Method for producing chlamydospore by trichoderma liquid deep fermentation
  • Method for producing chlamydospore by trichoderma liquid deep fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Preparation of slant culture medium: Take 20g of peeled potatoes and boil for 20 minutes to get the juice, mix the juice with 2g of glucose, 1.5g of agar and water to make 100ml, pH6.8-7.0, put it into 2 eggplant bottles, each 50ml each, make slopes after sterilization.

[0043] Preparation of medicine bottle medium: prepare 12 bottles of shake flask medium, the shake flask is a 500ml Erlenmeyer flask, the filling volume is 100ml / bottle, and the medium composition of each bottle is 2g starch, 1g glucose, 1g yeast powder, 0.3g CaCO 3, add water to 100ml, natural pH value, moist heat sterilization.

[0044] Preparation of seed tank culture medium: the size of the seed tank is 1 m 3 , The medium loading capacity is 500L. The content of each component of the medium is: starch 5kg, glucose 5kg, yeast powder 2.5kg, CaCO 3 1.5kg, add water to 500ml, pH6.5-6.8, steam sterilization.

[0045] Preparation of fermenter culture medium: the size of the fermenter is 10m 3 , mediu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention is fermentation culture medium formula and fermentation process of deep liquid ventilating fermentation of trichoderma to produce chlamydospore. The present invention has obviously shortened fermentation period, greatly increased chlamydospore yield, and greatly lowered trichoderma fermenting production cost. Furthermore, the chlamydospore produced based on the present invention has vigor and resistance higher than that of chlamydospore produced in traditional method, and is even suitable for production application.

Description

technical field [0001] The invention relates to a method for producing chlamydospores by liquid submerged fermentation of Trichoderma. technical background [0002] Trichoderma (Trichoderma spp.) belongs to the subphylum Deuteromycotina, Hyphophyceae, Synchospora, Myxosporum, is a kind of ubiquitous saprophytic fungi, and is the most potential plant disease biological control strain among fungi , can be used to control a variety of plant fungal diseases. The common species of Trichoderma are T.harzianum, T.hamatum, T.polysorum, T.koningii, Pseudomonas Mold (T.pseudokoningii), Trichoderma viride (T.viride) and Trichoderma longibrachiatum (T.longibrachiatum), etc. Among many species, Trichoderma viride (T.viride) and Trichoderma harzianum (T.harzianum) are particularly prominent in the control and control of plant diseases, and are currently the main strains used in production. [0003] Trichoderma can produce three propagules during its growth cycle, including mycelium, ch...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/14A01N63/04C05F11/08
Inventor 蒋细良孙连壮朱昌雄田云龙郭萍张凤英
Owner HAINAN JINYUFENG BIOLOGICAL ENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com