Method for producing chlamydospore by trichoderma liquid deep fermentation

A technology for chlamydospore and Trichoderma, which is applied in the directions of botanical equipment and methods, fungi, biocides, etc., can solve the problems of long fermentation cycle, high fermentation cost, low fermentation yield, etc. The effect of cost reduction and fermentation cost reduction

Active Publication Date: 2006-08-23
HAINAN JINYUFENG BIOLOGICAL ENG
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Problems solved by technology

Because the fermentation cycle is too long, the fermentation yield is low, and the ferment

Method used

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  • Method for producing chlamydospore by trichoderma liquid deep fermentation
  • Method for producing chlamydospore by trichoderma liquid deep fermentation
  • Method for producing chlamydospore by trichoderma liquid deep fermentation

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Embodiment 1

[0042] Preparation of slant culture medium: Take 20g of peeled potatoes and boil for 20 minutes to get the juice, mix the juice with 2g of glucose, 1.5g of agar and water to make 100ml, pH6.8-7.0, put it into 2 eggplant bottles, each 50ml each, make slopes after sterilization.

[0043] Preparation of medicine bottle medium: prepare 12 bottles of shake flask medium, the shake flask is a 500ml Erlenmeyer flask, the filling volume is 100ml / bottle, and the medium composition of each bottle is 2g starch, 1g glucose, 1g yeast powder, 0.3g CaCO 3, add water to 100ml, natural pH value, moist heat sterilization.

[0044] Preparation of seed tank culture medium: the size of the seed tank is 1 m 3 , The medium loading capacity is 500L. The content of each component of the medium is: starch 5kg, glucose 5kg, yeast powder 2.5kg, CaCO 3 1.5kg, add water to 500ml, pH6.5-6.8, steam sterilization.

[0045] Preparation of fermenter culture medium: the size of the fermenter is 10m 3 , mediu...

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Abstract

The present invention is fermentation culture medium formula and fermentation process of deep liquid ventilating fermentation of trichoderma to produce chlamydospore. The present invention has obviously shortened fermentation period, greatly increased chlamydospore yield, and greatly lowered trichoderma fermenting production cost. Furthermore, the chlamydospore produced based on the present invention has vigor and resistance higher than that of chlamydospore produced in traditional method, and is even suitable for production application.

Description

technical field [0001] The invention relates to a method for producing chlamydospores by liquid submerged fermentation of Trichoderma. technical background [0002] Trichoderma (Trichoderma spp.) belongs to the subphylum Deuteromycotina, Hyphophyceae, Synchospora, Myxosporum, is a kind of ubiquitous saprophytic fungi, and is the most potential plant disease biological control strain among fungi , can be used to control a variety of plant fungal diseases. The common species of Trichoderma are T.harzianum, T.hamatum, T.polysorum, T.koningii, Pseudomonas Mold (T.pseudokoningii), Trichoderma viride (T.viride) and Trichoderma longibrachiatum (T.longibrachiatum), etc. Among many species, Trichoderma viride (T.viride) and Trichoderma harzianum (T.harzianum) are particularly prominent in the control and control of plant diseases, and are currently the main strains used in production. [0003] Trichoderma can produce three propagules during its growth cycle, including mycelium, ch...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04C05F11/08
Inventor 蒋细良孙连壮朱昌雄田云龙郭萍张凤英
Owner HAINAN JINYUFENG BIOLOGICAL ENG
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