Unlock instant, AI-driven research and patent intelligence for your innovation.

Transgene mouse model of poly glumine disease, its culturing method and medicine for treating the disease

A technology of polyglutamine and transgenic mice, which is applied in the field of life sciences and can solve problems such as the unclear role of DRPLA

Inactive Publication Date: 2006-10-04
FUDAN UNIV
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, the effect of these compounds on DRPLA is still unclear.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Transgene mouse model of poly glumine disease, its culturing method and medicine for treating the disease
  • Transgene mouse model of poly glumine disease, its culturing method and medicine for treating the disease
  • Transgene mouse model of poly glumine disease, its culturing method and medicine for treating the disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0126] 1. Molecular biology experiments

[0127] 1) DNA cloning

[0128] All the plasmids were amplified with DH5α as the host bacteria, and the method of plasmid extraction refers to molecular cloning. Restriction endonucleases from Biolab Company and T4 DNA ligase from Takara Company were used to digest and connect the plasmids according to the recommended procedures.

[0129] 2) PCR

[0130] 10x PCR Buffer: 166mM (NH 4 ) 2 SO 4 , 670mM Tris (pH 8.8), 67mM MgCl 2 , 50mM beta-mercaptoethanol, 67μM EDTA.

[0131] PCR Reaction: DNA template, Primer, 10xBuffer, DMSO(10x), dNTP, Taq, H 2 O.

[0132] Thermofile: 93°C 90 sec, (93°C 30sec, 57°C 30sec, 65°C 3min) x 40cycles, 65°C 10min, 4°C max.

[0133] 3) DNA extraction and Southern hybridization

[0134] Take 1-2cm of mouse tail and place it in 1.5ml lysis buffer (100mM NaCl, 100mM Tris (pH 8.0), 25mM EDTA, 0.5% (w / v) SDS), add 20μl proteinase K (10mg / ml), and lyse at 55℃ More than 8 hours, intermittent shaking. The ly...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

This invention relates to a kind of polyglutamine disease (DRPLA) transgenosis mouse model, culture method and drug for treating the disease. DRPLA is a nerve regression disease caused by glutamine's repeat sequence CAG expanding unconventionally. The invention establishes DRPLA transgenosis mouse model, achieves cultivate method of the model, and obtains a drug for curing the DRPLA disease, accordingly the intention of curing DRPLA disease is achieved.

Description

technical field [0001] The invention belongs to the field of life sciences, and specifically relates to a transgenic mouse model of polyglutamine disease, a breeding method and a medicine for treating the disease. technical background [0002] Polyglutamine disease, or DRPLA, is a type of neurodegenerative disease caused by the abnormal expansion of the CAG repeat sequence encoding glutamine in the pathogenic gene. So far, nine polyglutamine diseases have been discovered, including : dentatorubral-pallidoluysian atrophy (DRPLA), Huntington disease (HD), spinobulbar muscular atrophy (SBMA) and six types of spinocerebellar ataxia (spinocerebellar ataxia, SCA1, 2, 3, 6, 7, 17), most of the patients with these nine diseases showed onset after middle age, progressive loss of neurological function, and neurological changes in specific brain regions appeared 10 to 20 years after the onset. Meta is missing. [0003] The disorders share some similar genetic and pathological propert...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K49/00A61K31/19A61P25/00A61P25/28A61P25/16
Inventor 许田应明耀韩珉吴晓晖徐人尔庄原朱化星
Owner FUDAN UNIV